Nuclear maturity of human spermatozoa selected by swim-up or by percoll gradient centrifugation procedures

Fertility and Sterility

Volumn 65, Issue 1, 1996, Pages 160-164

  Colleu, D ^a   Lescoat, D ^a   Gouranton, J ^a  

^a LHEG   (France)

Author keywords

electrophoresis; Human sperm; nuclear maturity; Percoll; swim up

Indexed keywords

HISTONE; NUCLEAR PROTEIN;

ARTICLE; CELL NUCLEUS; CLINICAL ARTICLE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; HUMAN; HUMAN CELL; MALE; MALE INFERTILITY; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROTEIN ELECTROPHORESIS; SPERMATOZOON COUNT; SPERMATOZOON DENSITY; SPERMATOZOON MATURATION; SPERMATOZOON MOTILITY;

EID: 0030026439     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(16)58045-5     Document Type: Article

References (21)

1. T. Puwaravutipanich S. Panyim The nuclear basic proteins of human testes and ejaculated spermatozoa Exp Cell Res 90 1975 153 158
2. N. Tanphaichitr P. Sobhon N. Taluppheth P. Chalermisarachai Basic nuclear proteins in testicular cells and ejaculated spermatozoa Exp Cell Res 117 1978 347 356
3. J.M. Bedford M.J. Bent H. Calvin Variations in the structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa J Reprod Fertil 33 1973 19 31
4. D. Le Lannou Y. Blanchard Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or by swim up procedure J Reprod Fertil 84 1988 551 556
5. T. Berger R.P. Marrs D.L. Moyer Comparison of techniques for selection of motile spermatozoa Fertil Steril 43 1985 268 273
6. J.F. Guérin C. Mathieu J. Lornage M.C. Pinatel D. Boulieu Improvement of survival and fertilizing capacity of human spermatozoa in an IVF programme by selection on discontinuous Percoll gradients Hum Reprod 4 1989 798 804
7. World Health Organization WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction 2nd ed 1987 The Press Syndicate of the University of Cambridge Cambridge
8. G. David J.P. Bisson F. Czyglik P. Jouannet C. Gernigon Anomalies morphologiques du spermatozoïde humain J Gynecol Obstet Biol Reprod (Paris) 4 1975 17 36
9. D. Lescoat D. Colleu D. Boujard D. Le Lannou Electrophoretic characteristics of nuclear proteins from human spermatozoa Arch Androl 20 1988 1 8
10. R.J. Aitken Assessment of sperm function for IVF Hum Reprod 3 1988 89 95
11. E. Johannisson R. Eliasson Ethidium bromide uptake by spermatozoa from fertile and infertile men Int J Androl 3 Suppl 1981 72 73
12. D. Colleu D. Lescoat D. Boujard D. Le Lannou Human spermatozoal nuclear maturity in normozoospermia and astheno-zoospermia Arch Androl 21 1988 155 162
13. Y. Blanchard D. Lescoat D. Le Lannou Anomalous distribution of nuclear basic proteins in round-headed human spermatozoa Andrologia 22 1990 549 555
14. P. Sautière A. Martinage D. Belaiche A. Arkhis P. Chevaillier Comparison of the amino-acid sequences of human protamines HP2 and HP3 and of intermediate basic nuclear proteins HPS1 and HPS2. Structural evidence that HPS1 and HPS2 are pro-protamines J Biol Chem 263 1988 11059 11062
15. A. Martinage P. Sautière D. Bélaïche P. Chevaillier Intermediate basic nuclear proteins HPS1 and HPS2 from human sperm are precursors of protamines HP2 and HP3 A. Aubry M. Marraud B. Vitoux Second forum on peptides 174 1989 Colloque INSERM/John Libbey Eurotext Ltd Paris 57 60
16. A. Martinage A. Arkhys E. Alimi P. Sautière P. Chevaillier Molecular characterization of nuclear basic protein HPI1, a putative precursor of human sperm protamines HP2 and HP3 Eur J Biochem 191 1990 449 451
17. A. Arkhis A. Martinage P. Sautière P. Chevaillier Molecular structure of human protamine P4 (HP4), a minor basic protein of human sperm nuclei Eur J Biochem 200 1991 387 392
18. E. Alimi A. Martinage A. Arkhis D. Belaiche P. Sautière P. Chevaillier Amino-acid sequence of the human intermediate basic protein 2 (HPI2) from sperm nuclei Eur J Biochem 214 1993 445 450
19. R. Balhorn S. Reed N. Tanphaichitr Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males Experientia 44 1988 52 55
20. I. Belokopytova E.I. Kostyleva A.N. Tomilin V.I. Vorob’ev Human male infertility may be due to a decrease of the protamine P2 content in sperm chromatin Mol Reprod Dev 34 1993 53 57
21. M. Gusse P. Sautière D. Bélaiche A. Martinage C. Roux J.P. Dadoune Purification and characterization of nuclear basic proteins of human sperm Biochim Biophys Acta 884 1986 124 134