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Volumn 118, Issue 4, 1996, Pages 921-922

A concerted mechanism for ethane hydroxylation by the particulate methane monooxygenase from Methylococcus capsulatus (bath)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; HYDROXYLATION; PARTICLES (PARTICULATE MATTER);

EID: 0030026199     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja953407q     Document Type: Article
Times cited : (90)

References (40)
  • 21
    • 85088542751 scopus 로고    scopus 로고
    • note
    • 11 12
  • 27
    • 3643146177 scopus 로고    scopus 로고
    • note
    • Incubations were performed for 12-15 mm at 45 °C in the presence of formaldehyde (5 μM) in 50 mM PIPES buffer, pH 7.1, total volume of 2.3 mL. Incubation with each isotopoiner was performed in duplicate.
  • 28
    • 3643123276 scopus 로고    scopus 로고
    • note
    • Calculation of the tritium distribution in the methyl/methylene/water positions based on unity and infinite isotope effects gives the range 3:2:1 to 3.1.0.
  • 29
    • 85088544907 scopus 로고    scopus 로고
    • note
    • 3 was quantified by scintillation counting (0.04 μCi. 0 5% overall conversion) Incubation was performed in duplicate.
  • 30
    • 3643105577 scopus 로고    scopus 로고
    • unpublished results
    • Zhu, M.; Chan, S. I., unpublished results.
    • Zhu, M.1    Chan, S.I.2
  • 34
    • 3643082660 scopus 로고    scopus 로고
    • note
    • 24 of 26, corresponding to 82% ee S configuration, in close agreement with the optical purity of the starting material (88% ee).
  • 37
    • 3643116016 scopus 로고    scopus 로고
    • note
    • This finding cannot be explained by any kinetic resolution due to the observed further oxidation of an intermediate alkoxy species, since both enantiomers of the substrate give the same result.
  • 38
    • 3643068056 scopus 로고    scopus 로고
    • note
    • Such a phenomenon, considered very unlikely, was excluded in the studies with both sMMOs but could not be probed with the pMMO.
  • 39
    • 85088544571 scopus 로고    scopus 로고
    • note
    • -1 29


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.