Nuclear pH gradient in mammalian cells revealed by laser microspectrofluorimetry

Journal of Cell Science

Volumn 109, Issue 1, 1996, Pages 257-262

  Seksek, Olivier ^a   Bolard, Jacques ^a  

^a CNRS   (France)

Author keywords

Intracellular pH; Laser microspectrofluorimetry; Nuclear membrane; Nuclear pH; Nuclear pore complex; SNARF 1

Indexed keywords

CALCEIN; DEXTRAN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CELL PH; CYTOSOL; DIFFUSION; ION TRANSPORT; LASER; MICROFLUOROMETRY; MICROINJECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; STAINING;

3T3 CELLS; ANIMALS; BENZOPYRANS; CELL LINE; CELL NUCLEUS; CHO CELLS; CRICETINAE; DEXTRANS; FLUORESCEINS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; LASERS; MAMMALS; MICE; MICE, INBRED BALB C; NAPHTHOLS; RHODAMINES; SPECTROMETRY, FLUORESCENCE;

ANIMALIA; MAMMALIA;

EID: 0030025574     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Al-Mohanna, F. A., Caddy, K. W. T. and Bolsover, S. R. (1994). The nucleus is insulated from large cytosolic calcium ion changes. Nature 367, 745-750.
2. Avison, M. J., Hetherington, H. P. and Shulman, R. G. (1986.) Applications of NMR to studies of tissue metabolism. Annu. Rev. Biophys. Biophys. Chem. 15, 377-402.
3. Bassnett, S., Reinsich, L. and Beebe, D. C. (1990) Intracellular pH measurement using single excitation-dual emission fluorescence ratios. Am. J. Physiol 258, C171-C178.
4. Blank, P. S., Silverman, H. S., Chung, O. Y., Hogue, B. A., Stern, M. D., Hansford, R. G., Lakatta, E. G. and Capogrossi, M. C. (1992). Cytosilic pH measurements in single carboxy-seminaphthorhodafluor-1. Am. J. Physiol. 263, H276-H284.
5. Bonner, W. F. (1978). Protein migration and accumulation in nuclei. In The Cell Nucleus. vol 6 (ed H Busch), pp 97-148. Academic Press, New York.
6. Bright, G. R., Fisher, G. W., Rogowska, J. and Taylor, D. L. (1987). Fluorescence ratio imaging microscopy: Temporal and spatial measurments of cytoplasmic pH J. Cell Biol 98, 717-724.
7. 2+ concentration in intact cells Cell Calcium 16, 259-268
8. Bustamante, J. O. (1994). Nuclear electrophysiology. J. Membr. Biol. 138, 105-112.
9. Chang, K.-P. and Hendricks, L. D. (1985) Laboratory cultivation and maintenance of Leishmania. In Leishmaniasis (ed. K-P. Chang and R S Bray), pp 213-246. Elsevier, Amsterdam.
10. Connor, J. A. (1994). Intracellular calcium mobilization by inositol 1,4,5-triphosphate: intracellular movements and compartimentalization. Cell Calcium 14, 185-200.
11. Dubbin, P. N., Cody, S. H. and Williams, D. A. (1993). Intracellular pH mapping with SNARF-1 and confocal microscopy pH gradients within single culturred cells. Micron 24, 581-586.
12. Gerson, D. F. (1982). The relation between intracellular pH and DNA synthesis rate in proliferating lymphocytes. In Intracellular pH: Its Measurement, Regulation and Utilization in Cellular Functions (ed. R. Nuccitelli and O. W. Deamer), pp. 375-383. Alan R. Liss, New York.
13. Gillot, I. and Whitaker, M. (1994). Calcium signals in and around the nucleus in sea urchin eggs. Cell Calcium 16, 269-278.
14. 2+] changes in nucleus and cytosol. Cell Calcium 16, 239-246.
15. Lin, C., Hajnoczky, G. and Thomas, A. P. (1994). Propagation of cytosolic calcium waves into the nuclei of hepatocytes. Cell Calcium 16, 247-258.
16. Kusumi, A., Tsuji, A., Murata, M., Sako, Y., Yoshizawa, A. C., Kagiwada, S., Hayakawa, T. and Ohnishi, S. (1991) Development of a streak-camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells. Biochemistry 30, 6517-6527.
17. Hernàndez-Cruz, A., Salaand, F. and Adams, P. R. (1990). Subcellular calcuim transients visualized by confocal microscopy in a voltage-clamped vertebrate neuron. Science 247, 858-862.
18. Matzke, A. J. M. and Matzke, M. A. (1991). The electrical properties of the nuclear envelope and their possible role m the regulation of eukaryotic gene expression. Bioelectrochem. Bioenerg. 25, 357-370.
19. Minamikawa, T., Takahashi, A. and Fujita, S. (1995). Differences in features of calcium transients between the nucleus and the cytosol in cultured heart muscle cells: Analyzed by confocal microscopy. Cell Calcium 17, 165-173.
20. Nieminen, A.-L., Gores, G. J., Darvour, T. L., Herman, B. and Lemasters, J. J. (1990). Toxic injury from mercuric chloride in rat hepatocytes. J. Biol. Chem. 265, 2399-2408.
21. Neylon, C. B., Hoyland, J., Mason, W. T. and Irvine, R. (1990). Spatial dynamics of intracellular calcium in agonist-stimulated vascular smooth muscle cells. Am J. Physiol 259, 675-677.
22. 2+ concentration in isolated rat liver nuclei. Proc. Nat. Acad. Sci. USA 86, 453-457
23. Nicotera, P., Zhivotovsky, B. and Orrenius, S. (1994) Nuclear calcium transport and the role of calcium in apoptosis. Cell Calcium 16, 279-288.
24. Payne, P. L. and Horowitz, S. B. (1980). The movement of material between the nucleus and the cytoplasm. Cell. Biol. 4, 299-338.
25. 31P nuclear magnetic resonance spectroscopy. J. Biol. Chem. 25, 1170-1177.
26. Roos, A. and Boron, W. F. (1981). Intracellular pH. Physiol. Rev. 61, 297-421.
27. Seksek, O., Henry-Toulmé, N., Sureau, F. and Bolard, J. (1991) SNARF-1 as an intracellular pH indicator in laser microspectrofluorimetry: a critical assessment. Anal. Biochem. 193, 49-54.
28. Silver, R. A., Whitaker, M. and Bolsover, S. R. (1992). Intracellular ion imaging using fluorescent dyes: artefacts and limits to resolution. Pflügers Arch 420, 595-602
29. Slayman, C. L., Moussatos, V. V. and Webb W. W. (1994). Endosomal accumulation of pH indicator dyes delivered as acetoxymethyl esters. J. Exp. Biol. 196, 419-438.
30. 1450 induction. Eur. Biophys. J. 18, 301-307.
31. Thomas, J. A., Buschbaum, R. N., Zimniak, A. and Racker, E. (1979). Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. Biochemistry 18, 2210-2218.
32. Waybill, M. N. and Yelamarty, R. V., Zhang, Y., Scaduto, R. C., LaNoue, K. F., Hsu, C. J., Smith, B. C., Tillotson, D. L., Yu, F. T. S. and Cheung, J. Y. (1991). Nuclear calcium gradients in cultured rat hepatocytes. Am. J. Physiol. 261, E49-E57.
33. Williams, D. A., Fogarty, K. E., Tsien, R. Y. and Fay, F. S. (1985). Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2. Nature 318, 558-561.
34. Williams, D. A., Becker, P. L. and Fay, F. S. (1988). Regional changes in calcium underlying contraction of single smooth muscle cells. Science 235, 1644-1648.
35. Winkler, M. M. (1982) Regulation of protein synthesis in sea urchin eggs by intracellular pH. In Intracellular pH: Its Measurement, Regulation and Utilization in Cellular Functions (ed. R. Nuccitelli and O. W Deamer ), pp. 325-340. Alan R. Liss, New York.