WR-1065 and radioprotection of vascular endothelial cells. I. Cell proliferation, DNA synthesis and damage

Radiation Research

Volumn 145, Issue 2, 1996, Pages 210-216

  Rubin, D B ^a   Drab, E A ^a   Kang, H J ^a   Baumann, F E ^a   Blazek, E R ^a  

^a RUSH MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 (3 AMINOPROPYLAMINO)ETHANETHIOL; BUTHIONINE SULFOXIMINE; GLUTATHIONE;

ANIMAL TISSUE; ARTICLE; CELL CYCLE; CELL PROLIFERATION; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; DNA SYNTHESIS; DRUG EFFECT; DRUG MECHANISM; NONHUMAN; PRIORITY JOURNAL; RADIATION INJURY; RADIATION PROTECTION; VASCULAR ENDOTHELIUM;

ANIMALS; CATTLE; CELL CYCLE; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; DNA DAMAGE; DNA REPLICATION; ENDOTHELIUM, VASCULAR; GAMMA RAYS; GLUTATHIONE; MALE; MERCAPTOETHYLAMINES; RADIATION-PROTECTIVE AGENTS;

EID: 0030024906     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.2307/3579176     Document Type: Article

References (46)

1. J. W. Hopewell, The importance of vascular damage in development of late radiation effects in normal tissues. In Radiation Biology in Cancer Research (R. E. Meyn and H. R. Withers, Eds.), pp. 449-459. Raven Press, New York, 1980.
2. H. M. Schuller, A. C. Smith, M. Gregg and M. R. Boyd, Sequential pathological changes induced in rats with the anti-cancer drug 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU). Exp. Lung Res. 9, 327-339 (1985).
3. U. S. Ryan, Endothelial Cells. CRC Press, Boca Raton, FL, 1988.
4. L. F. Fajardo, The unique physiology of endothelial cells and its implications in radiobiology. In Frontiers in Radiation Therapy and Oncology, Vol. 23 (J. M. Vaeth and J. L. Meyer, Eds.), pp. 96-112. Karger, Basel, 1989.
5. J. W. Hopewell, W. Calvo, D. Campling, H. S. Reinhold, M. Rezvani and T. K. Yeung, Effects of radiation on the microvasculature, Implications for normal tissue damage. In Frontiers in Radiation Therapy and Oncology, Vol. 23 (J. M. Vaeth and J. L. Meyer, Eds.), pp. 85-95. Karger, Basel, 1989.
6. I. Y. R. Adamson and D. H. Bowden, Endothelial injury and repair in radiation-induced pulmonary fibrosis. Am. J. Pathol. 112, 224-230 (1983).
7. M. E. Warfield,. M. J. Schneidkraut, P. W. Ramwell and P. A. Kot, WR2721 ameliorates the radiation-induced depression in reactivity of rat abdominal aorta to U46619. Radiat. Res. 121, 63-66 (1990).
8. M. M. Dunn, E. A. Drab and D. B. Rubin, Effect of irradiation of endothelial cells-polymorphonuclear leukocyte interaction. J. Appl. Physiol. 60, 1932-1937 (1986).
9. P. E. Brown, Mechanism of action of aminothiol radioprotectors. Nature 211, 363-364 (1967).
10. L. M. Schuchter and J. H. Glick, The current status of WR-2721: A chemotherapy and radiation therapy protector. Bio. Ther. Cancer 3, 1-10 (1993).
11. 2, and the combination of both agents. Radiat. Res. 111, 361-373 (1987).
12. I. Wyatt, R. B. Moore and L. L. Smith, Competition for polyamine uptake into rat lung slices by WR2721 and analogues. Int. J. Radiat. Biol. 55, 463-472 (1989).
13. E. L. Travis, R. A. Newman and S. J. Helbing, WR 2721 modification of type II cell and endothelial cell function in mouse lung after single doses of radiation. Int. J Radiat. Oncol. Biol. Phys. 13, 1355-1359 (1987).
14. D. Murray, R. Simpson, E. Rosenberg, A. Carraway and R. Britten, Correlation between gamma-ray-induced DNA double-strand breakage and cell killing after biologically relevant doses: analysis by pulsed-field gel electrophoresis. Int. J. Radiat. Biol. 65, 419-426 (1994).
15. J. A. Aguilera, G. L. Newton, R. C. Fahey and J. F. Ward, Thiol uptake by Chinese hamster V79 cells and aerobic radioprotection as a function of net charge on thiol. Radiat. Res. 130, 194-204 (1992).
16. S. Zheng, G. L. Newton, G. Gonick, R. C. Fahey and J. F. Ward, Radioprotection of DNA by thiols: Relationship between the net charge on a thiol and its ability to protect DNA. Radiat. Res. 114, 11-27 (1988).
17. D. J. Grdina, B. Nagy and P. J. Meechan, Effect of an aminothiol (WR-1065) on radiation-induced mutagenesis and cytotoxicity in two repair-deficient mammalian cell lines. In Anticarcinogenesis and Radiation Protection: Strategies in Protection from Radiation and Cancer (O. F. Nygaard and A. C. Upton, Eds.), pp. 287-295. Plenum Press, New York, 1991.
18. D. Murray, S. C. vanAnkeren, L. Milas and R. E. Meyn, Radioprotective action of WR-1065 on radiation-induced DNA strand breaks in cultured Chinese hamster ovary cells. Radiat. Res. 113, 155-170 (1988).
19. R. D. Issels, S. Bourier, J. E. Biaglow, L. E. Gerweck and W. Wilmanns, Temperature-dependent influence of thiols upon glutathione in Chinese hamster ovary cells at cytotoxic concentrations. Cancer Res. 45, 6219-6224 (1985).
20. R. D. Issels, A. Nageie, K. G. Eckert and W. Wilmanns, Promotion of cystine uptake and its utilization for glutathione biosynthesis induced by cysteamine and N-acetylcysteine. Biochem. Pharmacol. 37, 881-888 (1988).
21. P. Wardman, M. F. Dennis, M. R. L. Stratford and J. White, Extracellular, intracellular and subcellular concentration gradients of thiols. Int. J. Radiat. Oncol. Biol. Phys. 22, 751-754 (1992).
22. D. B. Rubin, E. A. Drab, W. F. Ward, L. J. Smith and S. M. Fowell, Enzymatic responses to radiation in cultured vascular endothelial and smooth muscle cells. Radiat. Res. 99, 420-432 (1984).
23. D. B. Rubin, E. A. Drab and K. D. Bauer, Endothelial cell subpopulations in vitro: cell volume, cell cycle, and radiosensitivity. J. Appl. Physiol. 67, 1585-1590 (1989).
24. D. B. Rubin, E. A. Drab, W. F. Ward and K. D. Bauer, Cell cycle changes and cytotoxicity in irradiated cultures of bovine aortic endothelial cells. Radiat. Res. 108, 206-214 (1986).
25. D. B. Rubin, E. A. Drab, W. F. Ward and K. D. Bauer, Cell cycle progression in irradiated endothelial cells cultured from bovine aorta. Radiat. Res. 116, 364-371 (1988).
26. E. J. Hall, Cell survival curves. In Radiobiology for the Radiologist, 3rd ed., pp. 17-38. J. B. Lippincott, New York, 1988.
27. J. H. Kim and T. C. Evans, Effects of x-irradiation on the mitotic cycle of Ehrlich ascites tumor cells. Radiat. Res. 21, 129-143 (1964).
28. K. W. Kohn, R. A. G. Ewig, L. C. Erickson and L. A. Zwelling, Measurement of strand breaks and cross-links by alkaline elution. In DNA Repair. A Laboratory Manual of Research Procedures (E. Friedberg and P. Hanawalt, Eds.), pp. 379-401. Marcel Dekker, New York, 1969.
29. E. R. Blazek and J. G. Peak, Filter elution assays for DNA damage: Practical and mechanistic significance of the DNA in the filter support wash. Radiat. Res. 130, 384-388 (1992).
30. F. Tietze, Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal. Biochem. 27, 502-522 (1969).
31. M. A. Baker, G. J. Cerniglia and A. Zaman, Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem. 190, 360-365 (1990).
32. T. Mori, M. Watanabe, M. Horikawa, P. Nikaido, H. Kimura, T. Aoyama and T. Sugahara, WR-2721, its derivatives and their radioprotective effects on mammalian cells in culture. Int. J. Radiat. Biol. 44, 41-53 (1983).
33. D. Murray, A. Prager and L. Milas, Radioprotection of cultured mammalian cells by aminothiols WR-1065 and WR-255591: Correlation between protection against DNA double-strand breaks and cell killing after γ radiation. Radiat. Res. 120, 154-163 (1989).
34. D. Murray, A. Prager, S. vanAnkeren, E. Altschuler, M. Kerr, N. Terry and L. Milas, Comparative effect of the thiols dithiothreitol, cysteamine, and WR-151326 on survival and on the induction of DNA damage in cultured Chinese hamster ovary cells exposed to gamma radiation. Int. J. Radiat. Biol. 58, 71-91 (1990).
35. D. J. Grdina, W. H. Guilford, C. P. Sigdestad and S. Giometti, Effects of radioprotectors on DNA damage and repair, proteins, and cell cycle progression. Pharmacol. Ther. 39, 133-137 (1988).
36. C. P. Sigdestad, B. L. Bergquist and D. J. Grdina, The effect of chemical radiation protectors on cell cycle progression after gamma or neutron irradiation. Cell Prolif. 24, 271-280 (1991).
37. 2 phase following exposure to WR-1065. Radiat. Res. 126, 223-228 (1991).
38. A. B. Pardee, G1 events and regulation of cell proliferation. Science 246, 603-608 (1989).
39. L. H. Hartwell and T. A. Weinert, Checkpoints: Controls that ensure the order of cell cycle events. Science 246, 629-634 (1989).
40. S. N. Mooteri, J. L. Podolski, E. A. Drab, T. J. Saclarides, J. M. Onoda, S. S. Kantak and D. B. Rubin, WR-1065 and radioprotection of vascular endothelial cells II. Morphology. Radiat. Res. 145, 217-224 (1996).
41. A. Prager, N. H. A. Terry and D. Murray, Influence of intracellular thiol and polyamine levels on radioprotection by aminothiols. Int. J. Radiat. Biol. 64, 71-81 (1993).
42. D. J. Grdina, N. Shigematsu, P. Dale, G. L. Newton, J. A. Aguilera and R. C. Fahey, Thiol and disulfide metabolites of the radiation protector and potential chemopreventive agent WR-2721 are linked to both its anti-cytotoxic and anti-mutagenic mechanisms of action. Carcinogenesis 16, 767-774 (1995).
43. A. T. Vaughan, D. J. Grdina, P. J. Meechan, A. E. Milner and D. J. Gordon, Conformational changes in chromatin structure induced by the radioprotective aminothiol, WR 1065. Br. J. Cancer 60, 893-896 (1989).
44. K. D. Held and J. E. Biaglow, Mechanisms for the oxygen radical-mediated toxicity of various thiol-containing compounds in cultured mammalian cells. Radiat. Res. 139, 15-23 (1994).
45. T. T. Lam, D. B. Rubin and E. A. Drab, The effect of dexamethasone on the cytotoxic and enzymatic response of cultured endothelial cells to radiation. Radiat. Res. 103, 240-250 (1985).
46. D. B. Rubin, E. A. Drab, A. M. Stone, T. L. Walden, Jr. and W. R. Hanson, The influence of exogenous eicosanoids on the radiation response of cultured bovine aortic endothelial cells. Radiat. Res. 125, 41-47 (1991).