Identification of a committed precursor for the mast cell lineage

Science

Volumn 271, Issue 5250, 1996, Pages 818-822

  Rodewald, Hans Reimer ^a   Dessing, Mark ^a   Dvorak, Ann M ^b   Galli, Stephen J ^b  

^a BASEL INSTITUTE FOR IMMUNOLOGY   (Switzerland)

^b BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; PROTEINASE; STEM CELL FACTOR; THY 1 ANTIGEN;

ANIMAL CELL; ARTICLE; CELL GRANULE; CELL LINEAGE; CELL POPULATION; FETUS; FETUS BLOOD; HEMATOPOIETIC STEM CELL; IMMUNOPHENOTYPING; MAST CELL; MOUSE; NONHUMAN; PERITONEUM MAST CELL; PRIORITY JOURNAL;

ANIMALIA; MURINAE; SIGANUS;

EID: 0030024349     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5250.818     Document Type: Article

References (51)

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48. Fetal mice were obtained from timed-pregnant females. The day of the vaginal plug was counted as day 0.5 of pregnancy. Blood was isolated from fetal mice as described (6) Briefly, the fetus was removed from the uterus without interruption of the umbilical cord and positioned on its back; jugular veins and cervical arteries were then cut with microdissection forceps. Fetal blood (∼20 μl) was collected from each fetus with a heparinized pipette tip and diluted into PBS (10 ml) and 5% FCS containing hepann (100 U/ml) (Roche, Basel, Switzerland) on ice. To remove aggregates and traces of tissue, we filtered FB through a polyester mesh (Estal monornesh, mesh size 40 μ,; SST, Thal, Switzerland). Subsequently, FB leukocytes were enriched by Percoll (Pharmacia, Uppsala, Sweden) density gradient (p ≥ 1.056 g/ml) certrifugation (6). For FACS separation, phycoerythrin-coupled 5a-8 (antibody to Thy-1; Caltag, South San Francisco, CA), biotinylated ACK-4 (antibody to c-Kit) [M. Ogawa et al., J. Exp. Med. 174, 63 (1991)], fluorescein isothiocyanate-labeled 145-2C11 (antibody to CD3) [O. Leo, M Foo, D. H. Sachs, L. E. Samelson, J. A Bluestone, Proc. Natl. Acad. Sci. U.S.A. 84, 1374 (1987)], and streptavidin-allophycocyanin (Molecular Probes, Eugene, OR) were used as described (6)
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50. v/+ × WB-W/+ parents (Japan-SLC, Japan).
51. We thank K. Kretzschmar for expert technical assistance; E. Schmidt (Mainz) for advice on mast cell staining, S.-I. Nishikawa (Kyoto University, Kyoto) for providing ACK-4 monoclonal antibody to c-Kit; E. Wagner and the Animal Facility staff (Basel) for their care in maintenance of mouse colonies, S. Meyer for expert cell sorter operation; P Fox for technical assistance with the electron microscopy; H. Stahlberger for artwork, B. Pfeiffer and H. Spalinger for photography, and P. Ghia, K. Kaijalainen, F. Melchers, and S. Takeda (Basel) and C Lantz and M. Tsai (Boston) for discussions and critical reading of the manuscript The Basel Institute for Immunology was founded and is supported by F. Hoffmann-La Roche, Basel, Switzerland. Supported by NIH grants AI-33372 (A M D.) and CA/AI-72074 and AI/CA-23990 (S.J.G.).