Synthetic glycogels for affinity electrophoresis: A facile and efficient method for investigating sugar-lectin interaction

Journal of Biochemistry

Volumn 119, Issue 2, 1996, Pages 367-371

  Ohmae, Masashi ^a   Suzuki, Sayuri ^a   Tokura, Seiichi ^a   Nishi, Norio ^a   Nishimura, Shin Ichiro ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Affinity electrophoresis; Fluorometric detection; Glycogel; Glycomonomer; Lectin sugar interaction

Indexed keywords

ACRYLAMIDE; CONCANAVALIN A; GLUCOPYRANOSIDE; LECTIN; N,N' METHYLENEBISACRYLAMIDE; POLYACRYLAMIDE GEL; SUGAR; WHEAT GERM AGGLUTININ;

ARTICLE; COVALENT BOND; ELECTROPHORESIS; FLUOROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; REDUCTION;

TRITICUM AESTIVUM;

EID: 0030024084     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021249     Document Type: Article

References (21)

1. Lee, Y.C. (1992) Perspective of glycotechnology: Carbohydrate recognition for better or worse. Trends Glycosci. Glycotech. 4, 251-261
2. Sharon, N. and Lis, H. (1989) Lectins as cell recognition molecules. Science 246, 227-234
3. Matsumoto, I., Jinbo, A., Kitagaki, H., Golovchenko-Matsumoto, A., and Seno, N. (1980) Detection of lectin-sugar interaction by ultraviolet difference spectroscopy. J. Biochem. 88, 1093-1096
4. Privat, J.-P., Delmonte, F., Mialonier, G., Bouchard, P., and Monsigny, M. (1974) Fluorescence studies of saccharide binding to wheat germ agglutinin (lectin). Eur. J. Biochem. 47, 5-14
5. Van Landschoot, A., G. Loontiens, F., M. Clegg, R., Sharon, N., and De Bruyne, K. (1977) Binding of 4-methylumbelliferyl N-acetyl-chitooligosaccharides to wheat germ agglutinin. Eur. J. Biochem. 79, 275-283
6. Nagata, Y. and Burger, M.M. (1974) Wheat germ agglutinin: Molecular characteristics and specificity for sugar binding. J. Biol. Chem. 249, 3116-3122
7. Kronis, K.A. and Carver, J.P. (1982) Specificity of isolectins of wheat germ agglutinin for sialyloligosaccharides: A 360-MHz proton nuclear magnetic resonance binding study. Biochemistry 21, 3050-3057
8. Borrenbasek, C. and Mattiasson, B. (1980) A structurally related binding properties of concanavalin A using differential scanning calorimetry. Eur. J. Biochem. 107, 67-71
9. Ohyama, Y., Kasai, K., Nomoto, H., and Inoue, Y. (1985) Frontal affinity chromatography of ovalbumin glycoasparagines on a concanavalin A-Sepharose column. J. Biol. Chem. 260, 6882-6887
10. Honda, S., Taga, A., Suzuki, K., Suzuki, S., and Kahehi, K. (1992) Determination of the association constant of monovalent mode protein-sugar interaction by capillary zone electrophoresis. J. Chromatogr. 597, 377-382
11. Kuhn, R., Frei, R., and Christen, M. (1994) Use of capillary affinity electrophoresis for the determination of lectin-sugar interaction. Anal. Biochem. 218, 131-135
12. Sudo, K. and Kanno, T. (1976) Properties of the amylase produced in carcinoma of the lung. Clin. Chim. Acta 73, 1-12
13. Bog-Hansen, T.C., Bjerrum, O.J., and Brogren, C.-H. (1977) Identification and quantification of glycoproteins by affinity electrophoresis. Anal. Biochem. 81, 78-87
14. Horejsi, V., Ticha, M., and Kocourek, J. (1977) Studies on lectins: XXXI. Determination of dissociation constants of lectin sugar complexes by means of affinity electrophoresis. Biochim. Biophys. Acta 499, 290-300
15. Horejsi, V. and Ticha, M. (1986) Qualitative and quantitative applications of affinity electrophoresis for the study of protein-ligand interactions: A review. J. Chromatogr. 376, 49-67
16. Nishimura, S.-I., Furuike, T., and Matsuoka, K. (1994) Preparation of glycoprotein models: Pendant-type oligosaccharide polymers. Methods Enzymol. 242, 235-246
17. Nishimura, S.-I., Furuike, T., Matsuoka, K., Maruyama, K., Nagata, K., Kurita, K., Nishi, N., and Tokura, S. (1994) Synthetic glycoconjugates. 4. Use of ω-(acrylamido)alkyl glycosides for the preparation of cluster glycopolymers. Macromolecules 27, 4876-4880
18. Matsuoka, K. and Nishimura, S.-I. (1995) Synthetic glycoconjugates. 5. Polymeric sugar ligands available for determining the binding specificity of lectins. Macromolecules 28, 2961-2968
19. Furuike, T., Nishi, N., Tokura, S., and Nishimura, S.-I. (1995) Synthetic glycoconjugates. 6. Preparation and biochemical evaluation of novel cluster-type glycopolymers containing Galβ(1→4) GlcNAc (N-acetyllactosamine) residue. Macromolecules 28, 7242
20. Nagata, K., Furuike, T., and Nishimura, S.-I. (1995) Fluorescence-labeled synthetic glycopolymers: A new type of sugar ligands of lectins. J. Biochem. 118, 278-284
21. Furuike, T., Nishi, N., Tokura, S., and Nishimura, S.-I. (1995) Synthesis of Novel Clustered Glycopolymers Containing Triantennary Glycosides of N-Acetyllactosamine. Chem. Lett., 823