Rapid PCR method for site-directed mutagenesis on double-stranded plasmid DNA

BioTechniques

Volumn 20, Issue 1, 1996, Pages 44-46

  Xu, X ^a   Kang, S H ^a   Heidenreich, O ^a   Li, Q ^a   Nerenberg, M ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; DOUBLE STRANDED DNA; INTERLEUKIN 6; PLASMID DNA;

ARTICLE; ENHANCER REGION; GENE SEQUENCE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; SITE DIRECTED MUTAGENESIS;

ANIMALS; BASE SEQUENCE; BIOTECHNOLOGY; DNA PRIMERS; GENETIC VECTORS; INTERLEUKIN-6; MICE; MICROSATELLITE REPEATS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS);

EID: 0030023148     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96201bm09     Document Type: Article

References (11)

1. Carter, P. 1987. Improved oligonucleotide-directed mutagenesis using M13 vectors, p 382-403. In R. Wu and L. Grossman (Eds.), Methods in Enzymology, Vol. 154. Academic Press, San Diego.
2. Dendorfer, U., P. Oettgen and T.A. Libermann. 1994. Multiple regulation elements in the interleukin-6 gene mediate induction by prostaglandins, cyclic AMP, and lipopolysaccharide. Mol. Cell. Biol. 14:4443-4454.
3. Deng, W.P. and J.A. Nickoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
4. Du, Z., A.D.A. Regier and R.C. Desrosiers. 1995. Improved recombinant PCR mutagenesis procedure that uses alkaline-denatured, plasmid template. BioTechniques 18:376-378.
5. Hutchisun, C.A., S. Phillips, M.H. Edgell, S. Gillam, P. Jahnke and M. Smith. 1978. Mutagenesis at a specific position in a DNA sequence. J. Biol. Chem. 253:6551-6560.
6. Jones, D.H. and B.H. Howard. 1991. A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction. BioTechniques 10:62-66.
7. Kunkel, T.A., J.D. Roberts and R.A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection, p. 367-382. In R. Wu and L. Grossman (Eds.), Methods in Enzymology, Vol. 154 Academic Press, San Diego.
8. Markvardsen, P., S.F. Lassen, T. V. Borchert and I.G. Clausen. 1995. Uracil-USE, an improved method for site-directed mutagenesis on double-stranded plasmid DNA. BioTechniques 18:370-372.
9. Stewart, G.S., S. Dovey and D.M. O'Rourke. 1988. A rapid method for site directed mutagenesis of plasmid DNA. BioTechniques 6:511-512.
10. Tanabe, O., S. Akira, T. Kamiya, G.G. Wong, T. Hirano and T. Kishimoto. 1988. Genomic structure of the murine IL-6 gene: high degree conservation of potential regulatory sequences between mouse and human. J. Immunol. 141:3875-3881.
11. Taylor, J.W., J. Ott and F. Eckstein. 1985. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res. 13:8765-8785.