Susceptibility to Leishmania major infection in interleukin-4-deficient mice

Science

Volumn 271, Issue 5251, 1996, Pages 987-990

  Noben Trauth, Nancy ^a   Kropf, Pascale ^b   Müller, Ingrid ^b  

^a JACKSON LABORATORY   (United States)

^b UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTERLEUKIN 4;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; GENE DISRUPTION; HELPER CELL; INFECTION SENSITIVITY; LEISHMANIA MAJOR; LEISHMANIASIS; MOUSE; MOUSE MUTANT; NONHUMAN; PRIORITY JOURNAL; STEM CELL;

EID: 0030023112     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5251.987     Document Type: Article

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45. H2 cytokines by enzyme-linked immunosorbent assay (ELISA) with paired mAbs {IFN-γ [S. S. Slade and J Langhome, Immunobiology 179, 353 (1989)], IL-10 (PharMingen), and IL-4, IL-5 (Endogen)} The detection limits were 1 U/ml for IFN-γ, 3 U/ml for IL-5, 7 pg/ml for IL-4, and 40 pg/ml for IL-10.
46. 2 analysis applied to a Poisson distribution.
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48. 32P]deoxycytidine triphosphate was included per reaction. Hall of the PCR products were run through a 2% agarose gel. Band intensities were quantitated with a Fujix Bio-imaging Analyzer 8AS100 equipped with MacBase software.
49. We thank F Fitch, J. Augers, and J Bluestone for their help, advice, and generosity in letting us use their FACScan, W. E. Paul for the gift of IL-4 mAb 11B11; D Shire, Sanofi Recherche, France, for primer sequences and plasmid pMus3; K. Geiger for help with the statistics; B. Ledermann for helpful discussions; and R. Etges for his comments on the manuscript Partially supported by funds from the University of Notre Dame (I.M and P.K.), NIH grant NIH-1R29 Al37636-01 (I.M.), and the American Cancer Society and United States Public Health Service (B. Knowles). N.N.-T was supported by a postdoctoral fellowship from the National Cancer Institute.