Detection of antigenic determinants in the Treponema pallidum membrane protein TmpA using overlapping synthetic peptides

Journal of Immunological Methods

Volumn 189, Issue 1, 1996, Pages 137-140

  Antoni, Guido ^a   Dal Maso, Grazia ^a   Berti, Brunilde ^a   Soldatini, Claudia ^a   Cocola, Francesco ^a  

^a DIESSE Diagnostica Senese Srl   (Italy)

Author keywords

Antigenic determinant; Membrane protein; Trepona pallidum

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL PROTEIN; SYNTHETIC PEPTIDE;

ANTIBODY DETECTION; ARTICLE; CLINICAL ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; PRIORITY JOURNAL; SERODIAGNOSIS; SYPHILIS; TREPONEMA PALLIDUM;

BACTERIA (MICROORGANISMS); TREPONEMA PALLIDUM;

EID: 0030022779     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00254-5     Document Type: Article

References (5)

1. Hansen, E.B., Pedersen, P.E., Schouls, L.M., Severin, E. and Van Embden, J.D.A. (1985) Genetic characterization and partial sequence determination of a Treponema pallidum operon expressing two immunogenic proteins in Escherichia coli. J. Bacteriol. 162, 1227.
2. Hopp, T.P. and Woods, K.R. (1981) Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. USA 78, 3824.
3. IJsselmuiden, O.E., Schouls, L.M., Stolz, E., Aelbers, G.N.M., Agterberger, C.M., Top, J. and Van Embden, J.D.A. (1989) Sensitivity and specificity of an enzyme-linked immunosorbent assay using recombinant DNA-derived Treponema pallidum protein TmpA for serodiagnosis of syphilis and the potential use of TmpA for assessing the effect of antibiotic therapy. J. Clin. Microbiol. 27, 152.
4. Norris, S.J. (1993) Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Microbiol. Rev. 57, 750.
5. Schouls, L.M., IJsselmuiden, O.E., Weel, J. and Van Embden, J.D.A. (1989) Overproduction and purification of Treponema pallidum recombinant-DNA-derived proteins TmpA and TmpB and their potential use in serodiagnosis of syphilis. Infect. Immun. 57, 2612.