Activation of recombinant human neutrophil procollagenase in the presence of doxycycline results in fragmentation of the enzyme and loss of enzyme activity

Arthritis and Rheumatism

Volumn 39, Issue 2, 1996, Pages 235-244

  Smith Jr , Gerald N ^a   Brandt, Kenneth D ^a   Hasty, Karen A ^b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1,10 PHENANTHROLINE; ANTISERUM; CALCIUM ION; COLLAGEN FIBER; DOXYCYCLINE; EDETIC ACID; EGTAZIC ACID; PROCOLLAGENASE; TRYPSIN; ZINC;

ARTICLE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INACTIVATION; IMMUNOBLOTTING; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ALPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID; CALCIUM; CHELATING AGENTS; COLLAGENASES; DOXYCYCLINE; ENZYME ACTIVATION; ENZYME PRECURSORS; HUMANS; MOLECULAR WEIGHT; NEUTROPHILS; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; TRYPSIN; ZINC;

EID: 0030022245     PISSN: 00043591     EISSN: None     Source Type: Journal    
DOI: 10.1002/art.1780390209     Document Type: Article

References (35)

1. Yu LP Jr, Smith GN Jr, Brandt KD, Myers SL, O'Connor BL, Brandt DA: Reduction of the severity of canine osteoarthritis by prophylactic treatment with oral doxycycline. Arthritis Rheum 35:1150-1159, 1992
2. Yu LP Jr, Smith GN Jr, Brandt KD, O'Connor BL, Myers SL: Therapeutic administration of doxycycline slows the progression of cartilage destruction in canine osteoarthritis (abstract). Trans Orthop Res Soc 18:724, 1993
3. Golub LM, Lee HM, Lehrer G, Nemiroff G, McNamara TF, Kaplan R, Ramamurthy NS: Minocycline reduces gingival collagenolytic activity during diabetes: preliminary observations and a proposed new mechanism of action. J Periodontal Res 18:516-526, 1983
4. Golub LM, Ramamurthy NS, McNamara TF, Gomes B, Wolff M, Casino AA, Kapoor A. Zambon J, Cinacio S, Schneir M, Perry H: Tetracyclines inhibit tissue collagenase activity: a new mechanism in the treatment of periodontal disease. J Periodontal Res 19:651-655, 1984
5. Golub LM, Wolff M, Lee HM, McNamara TF, Ramamurthy NS, Zambon J, Ciancio S: Further evidence that tetracyclines inhibit collagenase activity in human crevicular fluid and from other mammalian sources. J Periodontal Res 20:12-23, 1985
6. Lauhio A, Sorsa T, Lindy O, Suomalainen K, Saari H, Golub LM, Konttinen YT: The anticollagenolytic potential of lymecycline in the long-term treatment of reactive arthritis. Arthritis Rheum 35:195-198, 1992
7. Seedor JA, Perry HD, McNamara TF, Golub LM, Buxton DF, Guthrie DS: Systemic tetracycline treatment of alkali-induced corneal ulceration in rabbits. Arch Ophthalmol 105:268-271, 1987
8. Greenwald RA, Moak SA, Ramamurthy NS, Golub LM: Tetracyclines suppress matrix metalloproteinase activity in adjuvant arthritis and in combination with flurbiprofen, ameliorate bone damage. J Rheumatol 19:927-938, 1992
9. Hasty KA, Hibbs MS, Kang AH, Mainardi CL: Secreted forms of human neutrophil collagenase. J Biol Chem 261:5645-5650, 1986
10. Hirose T, Patterson C. Pourmotabbed T, Mainardi CL, Hasty KA: Structure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity. Proc Natl Acad Sci USA 90:2569-2573, 1993
11. Smith GN Jr, Brandt KD, Hasty KA: Procollagenase is reduced to inactive fragments upon activation in the presence of doxy-cycline. Ann N Y Acad Sci 782:436-438, 1994
12. Maeda S, Kawai T, Okinata M, Fujiwara T, Horiuchi T, Saeki Y, Sato Y, Furasawa M: Production of human alpha interferon in silkworm using a baculovirus vector. Nature 315:592-594, 1985
13. Woessner JF, CJ Taplin: Purification and properties of a small latent matrix metalloproteinase of the rat uterus. J Biol Chem 263:16918-16925, 1988
14. Nagai Y, LaPierre CM, Gross J: Tadpole collagenase: preparation and purification. Biochemistry 5:3123-3130, 1966
15. Cawston TE, Barrett, AJ: A rapid and reproducible assay for collagenase using (1-14C) acetylated collagen. Anal Biochem 99:340-345, 1979
16. Weingarten H, Martin R, Feder J: Synthetic substrates of vertebrate collagenase. Biochemistry 24:6730-6734, 1985
17. Weingarten H, Feder J: Spectrophotometric assay for vertebrate collagenase. Anal Biochem 147:437-440, 1985
18. Green GDJ, Shaw E: Thiobenzyl benzyloxycarbonyl-L-lysinate: substrate for a sensitive colorimetric assay for trypsinlike enzymes. Anal Biochem 93:223-226, 1979
19. Glantz SA: Primer of Biostatistics. Third edition. New York, McGraw-Hill, 1992
20. Burns FR, Stack MS, Gray RD, Paterson CA: Inhibition of purified collagenase from alkali-burned rabbit corneas. Invest Ophthalmol Vis Sci 30:1569-1575, 1989
21. ++ in the action of mammalian collagenases. Arch Biochem Biophys 173:355-3610, 1976
22. Swann JC, Reynolds JJ, Galloway WA: Zinc metalloenzyme properties of active and latent collagenase from rabbit bone. Biochem J 195:41-49, 1981
23. Housley TJ, Baumann AP, Braun ID, Davis G, Seperack PK, Wilhelm SM: Recombinant Chinese hamster ovary cell matrix metalloprotease-3 (MMP-3, stromelysin-1): role of calcium in promatrix metalloprotease-3 (pro-MMP-3, prostromelysin-1) activation and thermostability of the low mass catalytic domain of MMP-3. J Biol Chem 268:4481-4487, 1993
24. Lowry CL, McGeehan G. Levine HL III: Metal ion stabilization of the conformation of a recombinant 19-kDa catalytic fragment of human fibroblast collagenase. Proteins 12:42-48, 1992
25. Yu LP Jr, Smith GN, Hasty KA, Brandt KD: Doxycycline inhibits type XI collagenolytic activity in human osteoarthritic cartilage. J Rheumatol 18:1450-1452, 1991
26. Seltzer JL, Jeffrey JJ, Eisen AZ: Evidence for mammalian collagenases as zinc ion metalloenzymes. Biochim Biophys Acta 485:179-187, 1977
27. Salowe SP, Marcy AI, Cuca GC, Smith CK, Kopka IE, Hagmann WK, Hermes JD: Characterization of the zinc-binding sites in human stromelysin-1: stoichiometry of the catalytic domain and identification of a cysteine ligand in the proenzyme. Biochemistry 31:4535-4540, 1992
28. Okada Y, Nagase H, Harris ED Jr: A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. J Biol Chem 261:14245-14255, 1986
29. Yu LP Jr, Smith GN Jr, Brandt KD, Capello W: Type XI collagen-degrading activity in human osteoarthritic cartilage. Arthritis Rheum 33:1626-1633, 1990
30. Mohtai M, Smith RL, Schurman DJ, Tsuji Y, Torti FM, Hutchinson NI, Stetler-Stevenson WG, Goldberg GI: Expression of 92-kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1. J Clin Invest 92:179-185, 1993
31. Flannery CR, Sandy JD: Identification of MMP-13 (collagenase-3) as the major matrix metalloproteinase expressed by chondrocytes during retinoic acid-induced matrix catabolism (abstract). Trans Orthop Res Soc 20:102, 1995
32. Sandy JD, Flannery CR, Neame PJ, Lohmander LS: The structure of aggrecan fragments in human synovial fluid: evidence for the involvement in osteoarthritis of a novel proteinase which cleaves the glu 373-ala 374 bond of the interglobular domain. J Clin Invest 89:1512-1516, 1992
33. Sandy JD, Neame PJ, Boynton RE, Flannery CR: Catobolism of aggrecan in cartilage explants: identification of a major cleavage site within the interglobular domain. J Biol Chem 266:894-902, 1991
34. Arsenis CA, Greenwald RA, Moak SA, Laskin RS: Inhibition of neutral metalloproteinase activities in epiphyseal and articular cartilages by tetracyclines, both in vivo and in vitro (abstract). Trans Orthop Res Soc 15:268, 1990
35. Fosang AJ, Last K, Neame PJ, Hughes CE, Caterson B, Hardingham TE, Knauper V, Murphy G, Tschesche H: Neutrophil collagenase cleaves at the aggreganase site in the interglobular region of aggrecan (abstract). Trans Orthop Res Soc 19:48, 1994