Covalently immobilized enzymes on biocompatible polymers for amperometric sensor applications

Biosensors and Bioelectronics

Volumn 11, Issue 4, 1996, Pages 365-373

  Doretti, Lucio ^a   Ferrara, Daniela ^a   Gattolin, Paola ^b   Lora, Silvano ^c  

^a CNR   (Italy)

^b Ist Polarografia ed E   (Italy)

^c Istituto di Fotochimica e Radiazioni d'Alta Energia   (Italy)

Author keywords

Biosensors; Choline oxidase; Covalent immobilization; Glucose oxidase; Radiation induced polymerization

Indexed keywords

AMPEROMETRIC SENSORS; BIOSENSORS; COMPOSITION EFFECTS; COPOLYMERS; ELECTROCHEMICAL ELECTRODES; ENZYME KINETICS; GAMMA RAYS; GLUCOSE; PLATINUM; POLYMERIC MEMBRANES; POLYMERIZATION;

CHOLINE OXIDASE; DIFFUSION LIMITING EFFECTS; ELECTROCHEMICAL RESPONSE; GLUCOSE OXIDASE; GLYCIDYL METHACRYLATE POLYMERS; HYDROXYETHYL; PLATINUM ELECTRODES;

ENZYME IMMOBILIZATION;

CHOLINE; CHOLINE OXIDASE; GLUCOSE; GLUCOSE OXIDASE; UNCLASSIFIED DRUG;

AMPEROMETRY; ARTICLE; BIOSENSOR; POLYMERIZATION;

EID: 0030021393     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/0956-5663(96)82732-1     Document Type: Article

References (20)

1. Albery, W.J. & Bartlett, P.N. (1985). Amperometric enzyme electrodes. Part I. theory. J. Electroanal. Chem., 194, 211-222.
2. Bartlett, P.N. (1990). Conducting organic salt electrodes. In: Biosensors: A Practical Approach, A.E.G. Cass (ed.). IRL Press, Oxford, pp. 47-95.
3. Bartlett, P.N., Bradford, V.Q. & Whitaker, R.G. (1991). Enzyme electrode studies of glucose oxidase modified with a redox mediator. Talanta, 38, 57-63.
4. Coulet, P.R. (1988). Biosensors-based analyzers: from design to instrument. In: Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses, G.G. Guilbault & M. Mascini (eds.). Reidel Publishing, Dordrecht, pp. 319-327.
5. Doretti, L., Ferrara, D., Barison, G. & Lora, S. (1994a). Glucose sensors based on enzyme immobilization onto biocompatible membranes obtained by radiation-induced polymerization. Appl. Biochem. Biotech., 49, 191-202.
6. Doretti, L., Ferrara, D. & Lora, S. (1993). Enzyme-entrapping membranes for biosensors obtained by radiation-induced polymerization. Biosensors & Bioelectronics, 8, 443-450.
7. Doretti, L., Gattolin, P. & Lora, S. (1994b). Amperometric choline sensor with enzyme immobilized by gamma-irradiation in a biocompatible membrane. Anal. Lett., 27, 2455-2470.
8. Gregg, B.A. & Heller, A. (1991). Redox polymer films containing enzyme electrodes. J. Phys. Chem., 95, 5976-5980.
9. Guilbault, G.G. (1984). In: Analytical Uses of Immobilized Enzymes. Marcel Dekker, New York.
10. Gursel, I. & Hasirci, V.N. (1992). Matrix entrapment of glucose oxidase by y irradiation. Biomaterials, 13, 150-155.
11. Hall, C.E. & Hall, E.A.H. (1993). Covalent immobilization of glucose oxidase on methacrylate copolymers for use in amperometric glucose sensor. Anal. Chim. Acta, 281, 645-653.
12. Hannibal-Friedrich, O., Chun, M. & Sernetz, M. (1980). Immobilization of β-galactosidase, albumin, and γ-globulin on epoxy-activated acrylic beads. Biotechnol. Bioeng., 22, 157-175.
13. Kàlal, J. (1978). Epoxy and aldehyde polymers as reagents. J. Polym. Sci. Polym. Symp., 62, 251-270.
14. 13C-NMR. J. Polym. Sci. Polym. Lett., 28, 307-314.
15. Nishikubo, T., Lizawa, T., Yamada, M. & Tsuchiga, K. (1983). Study of photopolymer XII. Synthesis of novel photosensitive polymers with pendant photosensitive group and photosensitizer groups. J. Polym. Sci. Polym. Chem., 21, 2025-2045.
16. Peppas, N.A., Moynihan, H.I. & Lucht, L.M. (1985). The structure of highly crosslinked poly(2-hydroxyethyl methacrylate) hydrogels. J. Biomed. Mater. Res., 19, 397-411.
17. Scheller, F. & Schubert, F. (1992). In: Biosensors. Elsevier Science Publishers, Amsterdam.
18. Sundberg, L. & Porath, J. (1974). Preparation of adsorbents for biospecific affinity chromatography. I. Attachment of group-containing ligands to insoluble polymers by means of functional oxiranes. J. Chromatogr., 90, 87-98.
19. Svec, F., Kàlal, J., Menyailova, I.I. & Nakhapetyan, L.A. (1978). Immobilization on (polyglycidyl methacrylate) Co(ethylene dimethacrylate) carrier and its derivatives. Biotechnol. Bioeng., 20, 1319-1328.
20. Winquist, F. & Danielsson, B. (1990). Semiconductor field effect devices. In: Biosensors: A Practical Approach, A.E.G. Cass (ed.). IRL Press, Oxford, pp. 171-190.