A new application of a peptide library to identify selective interaction between small peptides in an attempt to develop recognition molecules toward protein surfaces

Tetrahedron Letters

Volumn 37, Issue 1, 1996, Pages 85-88

  Sasaki, Shigeki ^a   Takagi, Manabu ^a   Tanaka, Yoshitsugu ^a   Maeda, Minoru ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DOPAMINE 2 RECEPTOR; PENTAPEPTIDE;

ARTICLE; BINDING AFFINITY; MAGNETISM; NUCLEAR MAGNETIC RESONANCE; PEPTIDE SYNTHESIS; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN IMMOBILIZATION; RECEPTOR BINDING; TECHNIQUE;

EID: 0030021370     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4039(95)02108-6     Document Type: Article

References (22)

1. Synthetic receptors for polar functional groups of proteins: a) LaBrenz, S. R.; Kelly, J. W., J. Am. Chem. Soc., 1995, 117, 1655,
2. b) Albert, J. S.; Goodman, M. S.; Hamilton, A. D., ibid, 1995, 117, 1143,
3. b) Tabe, M; Labroo, V; Sheppard, P; Sasaki, T; ibid, 1993, 115, 3866,
4. c) Voyer, N; Deschênes, D.; Bernier, J.; Roby, J; J. Chem. Soc., Chem. Commun. 1992, 134, and references cited therein.
5. Some hypotheses to predict recognition molecules based on the target sequences. Bost, K. L.; Smith, E. M.; Blalock, J. E., Proc. Natl. Acad. Sci. USA, 1985, 82, 1372, Araga, S.; Le Boeuf, R. D.; Blalock, J. E., ibid., 1993, 90, 8747,
6. Some hypotheses to predict recognition molecules based on the target sequences. Bost, K. L.; Smith, E. M.; Blalock, J. E., Proc. Natl. Acad. Sci. USA, 1985, 82, 1372, Araga, S.; Le Boeuf, R. D.; Blalock, J. E., ibid., 1993, 90, 8747,
7. b) Ghiso, J.; Saball, E.; Frangione, B., ibid., 1990, 87, 1288,
8. c) de Gasparo, M.; Whitebread, S.; Einsle, K.; Heusser, C., Biochem. J., 1989, 261, 310.
9. Peptide ligands for a small peptide-based receptor have been identified by combinatorial technologies, Boyce, R.; Li, G.; Nestler, H. P.; Suenaga, T.; Still, C.; J. Am. Chem. Soc., 1994, 116, 7955.
10. Lam, K. S.; Salmon, S. E.; Knapp, R. J., Nature, 1991, 354, 82. And a recent review on combinatorial technologies; Callop, M. A.; Barrett, R. W.; Dower W. J.; Fodor, S. P. A.; Gordon, E. M., J. Med. Chem., 1994, 37, 1233.
11. Lam, K. S.; Salmon, S. E.; Knapp, R. J., Nature, 1991, 354, 82. And a recent review on combinatorial technologies; Callop, M. A.; Barrett, R. W.; Dower W. J.; Fodor, S. P. A.; Gordon, E. M., J. Med. Chem., 1994, 37, 1233.
12. s protein, respectively, but functions of the extracellular region have not been elucidated.
13. Chloromethylated polystyrene-divinyl benzene (2%, 100-200 mesh; Cl: 0.96 mmol/g) beads were used.
14. The single sequence constructed on one library bead and the magnetic beads were evidenced with an amino acid sequencer or by amino acid analysis, respectively.
15. Long Chain Alkyl Amino-MPG (500 Å, 5 μ), CPG INC (New Jersey, U.S.A.).
16. A recent example, Lonneborg, A., Sharma, P., Stougaard P., PCR - Methods and Applications, 1995, 4, S168.
17. 5 beads with different sequences) and 7.6 mg (0.64 μmol) of magnetic beads were used.
18. Ionic strength of the PBS solution was adjusted with NaCl to 0.14 M.
19. 9) contained 6-7 μM of the nonapeptide (1) was used for UV measurements at 25°C.
20. The total concentration was kept at 20 μM using 1 and 4, and the complexation was monitored by UV spectrometer at 30°C.
21. The data were analyzed by the curve fitting method. Wilcox, C. S.; Cowart M. D., Tetrahedron Lett., 1986, 27, 5563
22. 1H-NMR measurements were done at 600 MHz at 30°C with presaturation of the water signal using a solution of 0.6 mM of 1. The signals were assigned based on the gmqcosy and gtnroesy spectra.