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Determination of one thousandth of an attomole (1 zeptomole) of alkaline phosphatase: Application in an immunoassay of proinsulln
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Bioluminescence Immunoassays for human endocrine hormones based on aqualite, a calcium-activated photoprotein
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Rigl T, Rivera HN, Patel MT, Ball RT, Stute NL, Smith DF: Bioluminescence Immunoassays for human endocrine hormones based on aqualite, a calcium-activated photoprotein. Clin Chem 1995, 41:1363-1364.
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0028073195
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0029154326
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Chemiluminescent detection of horseradish peroxidase by enzymatic generation of acridinium esters
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Akhavan-Tafti H, Sugioka K, Arghavani Z, DeSilva R, Handley RS, Sugioka Y, Eickholt RA, Perkins MP, Schapp AP: Chemiluminescent detection of horseradish peroxidase by enzymatic generation of acridinium esters. Clin Chem 1995, 41:1368-1369. This paper describes the application of acridinium esters for signalling, not as labels themselves, but when produced enzymatically by HRP. Thus, the advantage of using an enzyme label is combined with the chemiluminescence of acridinium esters, which produce a more long-lived signal because they are generated during detection.
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Akhavan-Tafti, H.1
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Novel nonseparation sandwich-type electrochemical enzyme immunoassay system for detecting marker proteins in undiluted blood
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Athey, D.1
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Multilabelling of ferrocenes to a glucose oxidase-digoxin conjugate for the development of a homogeneous electroenzymatic immunoassay
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0029052050
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Specific binding assay for biotin based on enzyme channelling with direct electron transfer electrochemical detection using horseradish peroxidase
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0029081402
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Amperometric detection of alkaline phosphatase activity at a horseradish peroxidase enzyme electrode based on activated carbon: Potential application to electrochemical immunoassay
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Enzyme Immunoassay to determine heavy metals using antibodies to specific metal-EDTA complexes: Optimization and validation of an immunoassay for soluble indium
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Chakraborti P, Hatcher FM, Blake RC, Ladd PA, Blake DA: Enzyme Immunoassay to determine heavy metals using antibodies to specific metal-EDTA complexes: optimization and validation of an immunoassay for soluble indium. Anal Biochem 1994, 217:70-75.
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0027866156
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Enzyme immunoassay based survey of precipitation and surface water for the presence of atrazine, metolachior and 2,4-D
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Hall JC, Van Deynze TD, Struger J, Chan CH: Enzyme immunoassay based survey of precipitation and surface water for the presence of atrazine, metolachior and 2,4-D. J Environ Sci Health 1993, 28:577-598.
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High-performance assays of small molecules: Enhanced sensitivity, rapidity, and convenience demonstrated with a noncompetitlve immunometric anti-immune complex assay system for digoxin
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Self CH, Dessi JL, Winger LA: High-performance assays of small molecules: enhanced sensitivity, rapidity, and convenience demonstrated with a noncompetitlve immunometric anti-immune complex assay system for digoxin. Clin Chem 1994, 40:2035-2041. Describes a high-performance anti-immune complex assay that extends the advantages of non-competitive immunometric assays to produce a rapid, sensitive, robust and operationally simple assay system for small molecular weight substances in serum. Digoxin is used as an example.
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0028968611
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Towbin H, Motz J, Oroszlan P, Zingel O: Sandwich immunoassay for the hapten angiotensin II. A novel assay principle based on antibodies against immune complexes. J Immunol Methods 1995, 181:167-176. Another application of anti-immune complex technology (see [17••]), in this case, for the small peptide angiotensin II. The sandwich immunossay is shown to have high sensitivity.
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Towbin, H.1
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0028923120
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A direct non-competitive idiometric enzyme immunoassay for serum oestradiol
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Mares A, De Boever J, Osher J, Quiroga S, Barnard G, Kohen F: A direct non-competitive idiometric enzyme immunoassay for serum oestradiol. J Immunol Methods 1995, 181:83-90. Reports an assay system based on the selective-antibody non-competitive approach technology approach to the assay of small molecules in serum. This 'idiometric' approach is demonstrated to provide a high-performance assay system for a steroid, that is appropriate for development as a dipstick method.
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Mares, A.1
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Enzyme immunometric assay for L-thyroxine using direct ultraviolet Irradiation
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Etienne E, Créminon C, Lamourette P, Grassi J, Pradelles P: Enzyme immunometric assay for L-thyroxine using direct ultraviolet Irradiation. Anal Biochem 1995, 225:34-38. L-thyroxine is captured onto antibody-coated plates, and subsequent exposure to UV light is then used to cause the formation of a covalent link. The covalently linked L-thyroxine is then quantified with the same antibody, which is now labelled with enzyme. Thus, the technique exploits UV irradiation to provide another approach to providing non-competitive assays for small molecules.
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Etienne, E.1
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Auriol, J.1
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Chemical synthesis of bispecific monoclonal antibodies; potential advantages in immunoassay systems
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Cook, A.G.1
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Reducing interference from heterophilic antibodies in a two-site immunoassay for carcinoembryonic antigen (CEA) by using a human/mouse chimerlc antibody to CEa as the tracer
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0028265491
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Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin
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Ezan E, Ducancel F, Gliet D, Drevet P, Menez A, Grognet JM, Boulain JC: Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin. J Immunol Methods 1994, 169:205-211. These authors produce a recombinant enzymatic tracer by inserting the rat prolactin DNA sequence into the amino-terminal end of the Escherichia coli ALP gene. In addition, recombinant immunogen, consisting of rat prolactin fused with two synthetic lgG binding domains derived from protein A, is used to raise polyclonal antibodies. Large amounts of fusion proteins are extracted from the culture media (see also [27]).
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Ezan, E.1
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0028941913
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Recombinant antibody-alkaline phosphatese conjugates for diagnosis of human IgGs: Application to anti-HBsAg detection
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Use of genetically engineered lipid-tagged antibody to generate functional europium chelate loaded liposomes. Application in fluoroimmunoassay
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