Microtubules and microtubule motors: Mechanisms of regulation

International Review of Cytology

Volumn 164, Issue , 1996, Pages 269-327

  Thaler, C D ^a   Haimo, L T ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cytoskeleton; Dynein; Kinesin; MAPS; Microtubules; Motility; Phosphorylation; Tubulin

Indexed keywords

ALPHA TUBULIN; CELL PROTEIN; CELL SURFACE RECEPTOR; CYTOPLASM PROTEIN; DYNEIN ADENOSINE TRIPHOSPHATASE; KINESIN; MICROTUBULE ASSOCIATED PROTEIN; PHOSPHATASE; PHOSPHOTRANSFERASE;

ANIMAL CELL; CELL CYCLE; CELL MOTILITY; CELL ORGANELLE; CENTROSOME; CONTROLLED STUDY; CYTOARCHITECTURE; CYTOSKELETON; DEPHOSPHORYLATION; FLAGELLUM; MICROTUBULE; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; REVIEW;

ANIMALIA;

EID: 0030020940     PISSN: 00747696     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0074-7696(08)62388-8     Document Type: Review

References (340)

1. K. Afshar N.R. Barton R.S. Hawley L.S.B. Goldstein DNA binding and meiotic chromosomal localization of the Drosophila nod kinesin-like protein Cell (Cambridge, Mass.) 81 1995 129 138
2. H. Aizawa Y. Sekine R. Takemura Z. Zhang M. Nangaku N. Hirokawa Kinesin family in murine central nervous system J. Cell Biol. 119 1992 1287 1296
3. V. Allan Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro. J. Cell Biol. 128 1995 879 891
4. V.J. Allan R.D. Vale Cell cycle control of microtubule-based membrane transport and tubule formation in vitro. J. Cell Biol. 113 1991 347 359
5. F. Aniento N. Emans G. Griffiths J. Gruenberg Cytoplasmic dynein-dependent vesicular transport from early to late endosomes J. Cell Biol. 123 1993 1373 1387
6. D.J. Asai S.M. Beckwith K.A. Kandl H.H. Keating H. Tjandra J.D. Forney The dynein genes of Paramecium tetraurelia. Sequences adjacent to the catalytic P-loop identify cytoplasmic and axonemal heavy chain isoforms J. Cell Sci. 107 1994 859 867
7. P.W. Baas T.P. Pienkowsk K.S. Kosik Processes induced by tau expression in Sf9 cells have an axonlike microtubule organization J. Cell Biol. 115 1991 1333 1344
8. J.T. Bagnara M.E. Hadley “Chromatophores and Color Change: The Comparative Physiology of Animal Pigmentation.” 1973 Prentice-Hall Englewood Cliffs, NJ.
9. K. Barkalow T. Hamasaki P. Satir Regulation of 22S dynein by a 29-kD light chain J. Cell Biol. 126 1994 727 735
10. M.C. Beckerle K.R. Porter Analysis of the role of microtubules and actin in erythrophore intracellular motility J. Cell Biol. 96 1983 354 362
11. L.D. Belmont A.A. Hyman K.E. Sawin T.J. Mitchison Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts Cell (Cambridge, Mass.) 62 1990 579 589
12. E. Berliner E.C. Young K. Anderson H.K. Mahtani J. Gelles Failure of a single-headed kinesin to track parallel to microtubule protofilaments Nature (London) 373 1995 718 721
13. R. Bernhardt A. Matus Light and electron microscopic studies of the distribution of microtubule-associated protein 2 in rat brain: A difference between the dendritic and axonal cytoskeletons J. Comp. Neurol. 226 1984 203 221
14. M. Bernstein P.L. Beech S.G. Katz J.L. Rosenbaum A new kinesin-like protein (Klp1) localized to a single microtubule of the Chlamydomonas flagellum J. Cell Biol. 125 1994 1313 1326
15. M. Bessen R.B. Fay G.B. Witman Calcium control of waveform in isolated flagellar axonemes of Chlaymdomonas. J. Cell Biol. 86 1980 446 455
16. L.I. Binder A. Frankfurter L.I. Rehbun The distribution of tau in the mammalian nervous system J. Cell Biol. 101 1985 1371 1378
17. S.M. Block L.S.B. Goldstein B.J. Schnapp Bead movement by single kinesin molecules studied with optical tweezers Nature (London) 348 1990 348 352
18. G.S. Bloom T.A. Schoenfeld R.B. Vallee Widespread distribution of the major polypeptide component of MAP1 (microtubule-associated protein 1) in the nervous system J. Cell Biol. 98 1984 320 330
19. G.S. Bloom M.C. Wagner K.K. Pfister S.T. Brady Native structure and physical properties of bovine brain kinesin and identification of the ATP binding subunit polypeptide Biochemistry 27 1988 3409 3416
20. M. Bomsel R. Parton S.A. Kuznetsov T.A. Schroer J. Gruenberg Microtubule- and motor-dependent fusion in vitro between apical and basolateral endocytic vesicels from MDCK cells Cell (Cambridge, Mass.) 62 1990 719 731
21. N.M. Bonini D.L. Nelson Differential regulation of Paramecium ciliary motility by cAMP and cGMP J. Cell Biol. 106 1988 1615 1624
22. N.M. Bonini D.L. Nelson Phosphoproteins associated with cyclic nuceotide stimulation of ciliary motility in Paramecium. J. Cell Sci. 95 1990 219 230
23. N.M. Bonini M.C. Gustin D.L. Nelson Regulation of ciliary motility by membrane potential in Paramecium. Cell Motil. Cytoskel. 6 1986 256 272
24. S.T. Brady A novel brain ATPase with properties expected for the fast axonal motor Nature (London) 317 1985 73 75
25. S.T. Brady K.K. Pfister G.S. Bloom A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm Proc. Natl. Acad. Sci. U.S.A. 87 1990 1061 1065
26. Bré M.-H. R. Pepperkok A.M. Hill N. Levilliers W. Ansorge E.H.K. Stelzer E. Karsenti Regulation of microtubule dynamics and nucleation during polarization in MDCK II cells J. Cell Biol. III 1990 3013 3021
27. C.J. Brokaw Control of flagellar bending: A new agenda based on dynein diversity Cell Motil. Cytoskel. 28 1994 199 204
28. C.J. Brokaw R. Kamiya Bending patterns of Chlamydomonas flagella: IV. Mutants with defects in inner and outer dynein arms indicate differences in arm function Cell Motil. Cytoskel. 8 1987 68 75
29. C.J. Brokaw S.M. Nagayama Modulation of the asymmetry of sea urchin sperm falgellar bending by calmodulin J. Cell Biol. 100 1985 1875 1883
30. C.J. Brokaw R. Josslin L. Bobrow Calcium ion regulation of flagellar beat symmetry in reactivated sea urchin spermatozoa Biochem. Biophys. Res. Commun. 58 1974 795 800
31. C.J. Brokaw D.J.L. Luck B. Huang Analysis of the movement of Chlamydomons flagella: The function of the radial-spoke system is revealed by comparison of wild-type and mutant flagella J. Cell Biol. 92 1982 722 732
32. K.D. Brown R.M.R. Coulson T.J. Yen D.W. Cleveland Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis J. Cell Biol. 125 1994 1303 1312
33. B. Brugg A. Matus Phosphorylation determines the binding of microtubule associated protein 2 (MAP2) to microtubules in living cells J. Cell Biol. 114 1991 735 743
34. B. Buendia G. Draetta E. Karsenti Regulation of the microtubule nucleating activity of centrosomes in Xenopus extracts: Role of cyclin A-associated protein kinase J. Cell Biol. 116 1992 1431 1442
35. J.C. Bulinski G.G. Borisy Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates J. Cell Biol. 87 1980 802 808
36. R.D. Burgoyne R. Cumming Ontogeny of microtubule-associate protein 2 in rat cerebellum: differential expression of the doublet polypeptides Neuroscience 11 1984 157 167
37. A. Caceres K.S. Kosik Inhibition of neurite polarity by tau antisense oligonucleotides in primary cerebellar neurons Nature (London) 343 1990 461 463
38. A. Caceres L.I. Binder M.R. Payne P. Bender L. Rehbun O. Steward Differential subcellular localizations of tubulin and the microtubule-associated protein MAP2 in brain tissue as revealed by immunohistochemistry with monoclonal hybridoma antibodies J. Neurosci. 4 1984 394 410
39. A. Caceres J. Mautino K.S. Kosik Suppression of MAP2 in cultured cerebellar macroneurons inhibits minor neurite formation Neuron 9 1992 607 618
40. V.A. Centonze G.G. Borisy Nucleation of microtubules from mitotic centrosomes is modulated by a phosphorylated epitope J. Cell Sci. 95 1990 405 411
41. r microtubule-associated protein (MAP4) contains a domain homologus to the microtubule-binding domains of neuronal MAP2 and tau J. Cell Sci. 98 1991 27 36
42. S.J. Chapin J.C. Bulinski Cellular microtubules heterogeneous in their content of microtubule-associated protein 4 (MAP4) Cell Motil. Cytoskel. 27 1994 133 149
43. J. Chen Y. Kanai N.J. Cowan N. Hirokawa Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons Nature (London) 360 1992 674 677
44. Y.-T. Chen M. Schliwa Direct observation of microtubule dynamics in Reticulomyxa: Unusual rapid length changes and microtubule sliding Cell Motil. Cytoskel. 17 1990 214 226
45. R.E. Cheney M.A. Riley M.S. Mooseker Phylogenetic analysis of the myosin superfamily Cell Motil. Cytoskel. 24 1993 215 223
46. T.J. Chilcote K.A. Johnson Phosphorylation of Tetrahymena 22S dynein J. Biol. Chem. 265 1990 17257 17266
47. S.W. Clark D.I. Meyer Centractin is an actin homologue associated with the centrosome Nature (London) 359 1992 246 250
48. S.W. Clark D.I. Meyer ACT3: A putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle J. Cell Biol. 127 1994 129 138
49. S.A. Cohn A.L. Ingold J.M. Scholey Correlation between the ATPase and microtubule translocating activities of sea urchin egg kinesin Nature (London) 328 1987 160 163
50. D.G. Cole S.W. Chinn K.P. Wedaman K. Hall T. Vuong J.M. Scholey Novel heterotrimeric kinesin-related protein purified from sea urchin eggs Nature (London) 366 1993 268 270
51. D.G. Cole W.M. Saxton K.B. Sheehan J.M. Scholey A “slow” homotetrameric kinesin-related motor protein purified from Drosophila embryos J. Biol. Chem. 269 1994 22913 22916
52. J. Condeelis Life at the leading edge: The formation of cell protrusions Annu. Rev. Cell Biol. 9 1993 411 444
53. I. Corthésy-Theulaz A. Pauloin S.R. Pfeffer Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex J. Cell Biol. 118 1992 1333 1345
54. M. Coue V.A. Lombillo J.R. McIntosh Microtubule depolymerization promotes particle and chromosome movement in vitro. J. Cell Biol. 112 1991 1165 1175
55. J.L. Cyr K.K. Pfister G.S. Bloom C.A. Slaughter S.T. Brady Molecular genetics of kinesin light chains: Generation of isoforms by alternative splicing Proc. Natl. Acad. Sci. U.S.A. 88 1991 10114 10118
56. S.L. Dabora M.P. Sheetz The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts Cell (Cambridge, Mass.) 54 1988 27 35
57. F.M. Davis T.Y. Tsao S.K. Fowler P.N. Rao Monoclonal antibodies to mitotic cells Proc. Natl. Acad. Sci. U.S.A. 80 1983 2926 2930
58. P. DeCamilli P.E. Miller F. Navone W.E. Theurkauf R.B. Vallee Distribution of microtubule-associated protein 2 in the nervous system of the rat studied by immunofluorescence Neuroscience 11 1984 817 846
59. C.S. Dey C.J. Brokaw Activation of Ciona sperm motility: Phosphorylation of dynein polypeptides and effects of a tyrosine kinase inhibitor J. Cell Sci. 100 1991 815 824
60. J.F. Dillman K.K. Pfister Differential phosphorylation in vivo of cytoplasmic dynein association with anterogradely moving organelles J. Cell Biol. 127 1994 1671 1681
61. J.H. Dinsmore F. Solomon Inhibition of MAP2 expression affects both morphological and cell division phenotypes of neuronal differentiation Cell 64 1991 817 826
62. S.A. Endow M.A. Titus Genetic approaches to molecular motors Annu. Rev. Cell Biol. 8 1992 29 66
63. S.A. Endow S. Henikoff L.S. Niedziela Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin Nature (London) 345 1990 81 83
64. S.A. Endow S.J. Kang L.L. Satterwhite M.D. Rose V.P. Skeen E.D. Salmon Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends EMBO J. 13 1994 2708 2713
65. A.P. Enos N.R. Morris Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell (Cambridge, Mass.) 60 1990 1019 1027
66. D. Eschel L.A. Urrestarazu S. Vissers J.-C. Jauniaux J.C. van Vliet-Reedijk R.J. Planta I.R. Gibbons Cytoplasmic dynein is required for normal nuclear segregation in yeast Proc. Natl. Acad. Sci. U.S.A. 90 1993 11172 11176
67. U. Euteneuer J.R. McIntosh Polarity of some motility-related microtubules Proc. Natl. Acad. Sci. U.S.A. 78 1981 372 376
68. S. Faruki M. Dorée E. Karsenti cdc2 kinase-induced destabilization of MAP2-coated microtubules in Xenopus egg extracts J. Cell Sci. 101 1992 69 78
69. K.R. Fath G.M. Trimbur D.R. Burgess Molecular motors are differentially distributed on Golgi membranes from polarized epithelial cells J. Cell Biol. 126 1994 661 675
70. C. Febvre-Chevalier J. Febvre Microtubule disassembly in vivo: Intercalary destabilization and breakdown of microtubules in the Heliozoan Actinocoryne contractilis. J. Cell Biol. 118 1992 585 594
71. F. Feiguin A. Ferreira K.S. Kosik A. Caceres Kinesin-mediated organelle translocation revealed by specific cellular manipulations J. Cell Biol. 127 1994 1021 1039
72. M.-A. Félix C. Antony M. Wright B. Maro Centrosome assembly in vitro: Role of gamma-tubulin recruitment in Xenopus sperm aster formation J. Cell Biol. 124 1994 19 31
73. L.A. Fox W.S. Sale Direction of force generated by the inner row of dynein arms on flagellar microtubules J. Cell Biol. 105 1987 1781 1787
74. L.A. Fox K.E. Sawin W.S. Sale Kinesin-related proteins in eukaryotic flagella J. Cell Sci. 107 1994 1545 1550
75. L.C. Gardner E. O'Toole C.A. Perrone T. Giddings M.E. Porter Components of a “dynein regulatory complex” are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella J. Cell Biol. 127 1994 1311 1325
76. V.I. Gelfand A.D. Bershadsky Microtubule dynamics: Mechanism, regulation, and function Annu. Rev. Cell Biol. 7 1991 93 116
77. B.H. Gibbons I.R. Gibbons The effect of partial extraction of dynein arms on the movement of reactivated-sea urchin sperm J. Cell Sci. 13 1973 337 357
78. B.H. Gibbons I.R. Gibbons Calcium-induced quiescence in reactivated sea urchin sperm J. Cell Biol. 84 1980 13 27
79. B.H. Gibbons D.J. Asai W.J. Tang T.S. Hays I.R. Gibbons Phytogeny and expression of axonemal and cytoplasmic dynein genes in sea urchins Mol. Cell. Biol. 5 1994 57 70
80. I.R. Gibbons Studies on the protein components of cilia from Tetrahymena pyriformis. Proc. Natl. Acad. Sci. U.S.A. 50 1963 1002 1010
81. I.R. Gibbons M.P. Cosson J.A. Evans B.H. Gibbons B.B. Houck K.H. Martinson W.S. Sale W.J. Tang Potent inhibition of dynein adenosine-triphosphatase and of motility of cilia and sperm flagella by vanadate Proc. Natl. Acad. Sci. U.S.A. 75 1978 2220 2224
82. I.R. Gibbons B.H. Gibbons G. Mocz D.J. Asai Multiple nucleotide-binding sites in the sequence of dynein beta heavy chain Nature (London) 352 1991 640 643
83. S.P. Gilbert K.A. Johnson Pre-steady-state kinetics of the microtubule-kinesin ATPase Biochemistry 33 1994 1951 1960
84. S.P. Gilbert M.R. Webb M. Brune K.A. Johnson Pathway of processive ATP hydrolysis by kinesin Nature (London) 373 1995 671 676
85. S.R. Gill T.A. Schroer I. Szilak E.R. Steuer M.P. Sheetz D.W. Cleveland Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein J. Cell Biol. 115 1991 1639 1650
86. S.E. Gitelman G.B. Witman Purification of calmodulin from Chlamydomonas: Calmodulin occurs in cell bodies and flagella J. Cell Biol. 98 1980 764 770
87. N.R. Glicksman S.F. Parsons E.D. Salmon Okadaic acid induces interphase to mitotic-like microtubule dynamic instability by inactivating rescue J. Cell Biol. 119 1992 1271 1276
88. L.S.B. Goldstein With apologies to Scheherazade: Tails of 1001 kinesin motors Annu. Rev. Genet. 27 1993 319 351
89. G.J. Gorbsky W.A. Ricketts Differential expression of a phosphoepitope at the kinetochore of moving chromosomes J. Cell Biol. 122 1993 1311 1321
90. N. Grundstrom J.O.G. Karlsson R.G.G. Andersson The control of granule movement in fish melanophores Acta Physiol. Scand. 125 1985 415 422
91. G.G. Gundersen M.H. Kalnoski J.C. Bulinski Distinct populations of microtubules: Tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo. Cell (Cambridge, Mass.) 38 1984 779 789
92. F.K. Gyoeva V.I. Gelfand Coalignment of vimentin intermediate filaments with microtubules depends on kinesin Nature (London) 353 1991 445 448
93. D.D. Hackney Kinesin ATPase: Rate-limiting ADP release Proc. Natl. Acad. Sci. U.S.A. 85 1988 6314 6318
94. D.D. Hackney The rate-limiting step in microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains occurs while bound to the microtubule J. Biol. Chem. 269 1994 16508 16511
95. D.D. Hackney Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis Proc. Natl. Acad. Sci. U.S.A. 91 1994 6865 6869
96. 2 forms of kinesin Biochem. Biophys. Res. Commun. 174 1991 810 815
97. D.D. Hackney J.D. Levitt J. Suhan Kinesin undergoes a 9S to 6S conformational transition J Biol. Chem. 267 1992 8696 8701
98. + gene Nature (London) 347 1990 563 566
99. H. Hagiwara H. Yorifuji R. Sato-Yoshitake N. Hirokawa Competition between motor molecules (kinesin and cytoplasmic dynein) and fibrous microtubule-associated proteins in binding to microtubules J. Biol. Chem. 269 1994 3581 3589
100. D.H. Hall E.M. Hedgecock Kinesin-related gene unc-104 is required for axonal transport of synaptic vesicles in C. elegans. Cell (Cambridge, Mass.) 65 1991 837 847
101. K. Hall D.G. Cole Y. Yeh J.M. Scholey R.J. Baskin Force-velocity relationships in kinesin-driven motility Nature (London) 364 1993 457 459
102. T. Hamasaki T.J. Murtaugh Satir B.H. P. Satir In vitro phosphorylation of Paramecium axonemes and permeabilized cells Cell Motil. Cytoskel. 12 1989 1 11
103. T. Hamasaki K. Barkalow J. Richmond P. Satir cAMP-stimulated phosphorylation of an axonemal polypeptide that copurifies with the 22S dynein arm regulates microtubule translocation velocity and swimming speed in Paramecium. Proc. Natl. Acad. Sci. U.S.A. 88 1991 7918 7922
104. S.F. Hamm-Alvarez P.Y. Kim M.P. Sheetz Regulation of vesicle transport in CV-1 cells and extracts J. Cell Sci. 106 1993 955 966
105. A. Harada K. Oguchi S. Okabe J. Kuno S. Terada T. Ohshima R. Sato-Yoshitake Y. Takel T. Noda N. Hirokawa Altered microtubule organization in small-calibre axons of mice lacking tau protein Nature (London) 369 1994 488 491
106. E. Hasegawa H. Hayashi S. Asakura R. Kamiya Stimulation of in vitro motility of Chlamydomonas axonemes by inhibition of cAMP-dependent phosphorylation Cell Motil. Cytoskel. 8 1987 302 311
107. M. Hatsumi S.A. Endow Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis J. Cell Sci. 103 1992 1013 1020
108. J.H. Hayden R.D. Allen Detection of single microtubules in living cells: Particle transport can occur in both directions along the same microtubule J. Cell Biol. 99 1984 1785 1793
109. M.M.S. Heck A. Pereira P. Pesavento Y. Yannoni A.C. Spradling L.S.B. Goldstein The kinesin-like protein KLP61F is essential for mitosis in Drosophila. J. Cell Biol. 123 1993 665 679
110. N. Hirokawa Microtubule organization and dynamics dependent on microubule-associated proteins Curr. Opin. Cell Biol. 6 1994 74 81
111. N. Hirokawa K.K. Pfister H. Yorifuji M.C. Wagner S.T. Brady G.S. Bloom Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration Cell (Cambridge, Mass.) 56 1989 867 878
112. N. Hirokawa R. Sato-Yoshitake T. Yoshida T. Kawashima Brain dynein (MAP1C) localizes on both anterogradely and retrogradely transported membranous organelles in vivo. J. Cell Biol. III 1990 1027 1037
113. N. Hirokawa R. Sato-Hoshitake N. Kobayashi K.K. Pfister G.S. Bloom S.T. Brady Kinesin associates with anterogradely transported membranous organelles in vivo. J. Cell Biol. 114 1991 295 302
114. C.J. Hogan H. Wein L. Wordeman J.M. Scholey K.E. Sawin W.Z. Cande Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain Proc. Natl. Acad. Sci. U.S.A. 90 1993 6611 6615
115. P.J. Hollenbeck The distribution, abundance and subcellular localization of kinesin J. Cell Biol. 108 1989 2335 2342
116. P.J. Hollenbeck Phosphorylation of neuronal kinesin heavy and light chains in vivo. J. Neurochem. 60 1993 2265 2275
117. P.J. Hollenbeck J.A. Swanson Radial extension of macrophage tubular lysosomes supported by kinesin Nature (London) 346 1990 864 866
118. E.L.F. Holzbaur R.B. Vallee Dyneins: Molecular structure and cellular function Annu. Rev. Cell. Biol. 10 1994 339 372
119. E.L.F. Holzbaur J.A. Hammarback B.M. Paschal N.G. Kravit K.K. Pfister R.B. Vallee Homology of a 150kD cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued. Nature (London) 351 1991 579 583
120. T. Horio H. Hotani Visualization of the dynamic instability of individual microtubules by dark-field microscopy Nature (London) 321 1986 605 607
121. T. Horio B.R. Oakley Human γ-tubulin functions in fission yeast J. Cell Biol. 126 1994 1465 1473
122. D.R. Howard G. Habermacher D.B. Glass E.F. Smith W.S. Sale Regulation of Chlamydomonas flagellar dynein by an axonemal protein kinase J. Cell Biol. 127 1994 1683 1692
123. J. Howard A.J. Hudspeth R.D. Vale Movement of microtubules by single kinesin molecules Nature (London) 342 1989 154 158
124. M.A. Hoyt Cellular roles of kinesin and related proteins Curr. Opin. Cell Biol. 6 1994 63 68
125. M.A. Hoyt L. He K.K. Loo W.S. Saunders Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly J. Cell Biol. 118 1992 109 120
126. M.A. Hoyt L. He L. Totis W.S. Saunders Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor mutations Genetics 135 1993 35 44
127. B. Huang Z. Ramanis D.J.L. Luck Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for flagellar function Cell (Cambridge, Mass.) 28 1982 115 124
128. A.J. Hunt F. Gittes J. Howard The force exerted by a single kinesin molecule against a viscous load Biophys. J. 67 1994 766 781
129. H.E. Huxley The mechanism of muscular contraction Science 164 1969 1356 1366
130. A.A. Hyman T.J. Mitchison Two different microtubule-based motor activities with opposite polarities in kinetechores Nature (London) 351 1991 206 211
131. A.A. Hyman K. Middleton M. Centola T.J. Mitchison J. Carbon Microtubule-motor activity of a yeast centromere-binding protein complex Nature (London) 359 1992 533 536
132. K.A. Johnson The pathway of ATP hydryolysis by dynein. Kinetics of a presteady state phosphate burst J. Biol. Chem. 258 1983 13825 13832
133. K.A. Johnson Pathway of the microtubule-dynein ATPase and structure of dynein: A comparison with actomyosin Annu. Rev. Biophys. Biophys. Chem. 14 1985 161 188
134. K.A. Johnson J.L. Rosenbaum Polarity of flagellar assembly in Chlamydomonas. J. Cell Biol. 119 1992 1605 1611
135. K.A. Johnson M.A. Haas J.L. Rosenbaum Localization of a kinesin-related protein to the central pair apparatus of the Chlamydomonas reinhardtii flagellum J. Cell Sci. 107 1994 1551 1556
136. H.C. Joshi M.J. Palacios L. McNamara D. Cleveland γ-tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation Nature (London) 356 1992 80 82
137. O. Kagami R. Kamiya Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein J. Cell Sci. 103 1992 653 664
138. R. Kamiya Extrusion and rotation of the central-pair microtubules in detergent-treated Chlamydomonas flagella Cell Motil, Suppl. 1 1982 169 173
139. R. Kamiya M. Okamoto A mutant of Chlamydomonas reinhardtii that lacks the flagellar outer dynein arm but can swim J. Cell Sci. 74 1985 181 191
140. Y.M. Kersey P.K. Hepler C.A. Palevitz N.K. Wessells Polarity of actin filaments in Characean algae Proc. Natl. Acad. Sci. U.S.A. 73 1976 165 168
141. S. Khawaja G.G. Gundersen J.C. Bulinski Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level J Cell Biol. 106 1988 141 150
142. S.M. King G.B. Witman Immunolocalization of an intermediate chain of outer arm dynein by immunoelectron microscopy J. Biol. Chem. 265 1990 19807 19811
143. S.M. King G.B. Witman Multiple sites of phosphorylation within the heavy chain of Chlamyodmonas outer arm dynein J. Biol. Chem. 269 1994 5452 5457
144. r 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with α-tubulin in situ J. Biol. Chem. 266 1991 8401 8407
145. J. Knops K.S. Kosik G. Lee J.D. Pardee L. Cohen-Gould L. McConlogue Overexpression of tau in a nonneuronal cell induces long cellular processes J. Cell Biol. 114 1991 725 733
146. S. Kondo R. Sato-Yoshitake Y. Noda H. Aizawa T. Nakata Y. Matsuura N. Hirokawa KIF3A is a new microtubule-based anterograde motor in the nerve axon J. Cell Biol. 125 1994 1109 1117
147. M.P. Koonce J.R. McIntosh Identification and immunolocalization of cytoplasmic dynein in Dictyostelium. Cell Motil. Cytoskel. 15 1990 51 62
148. M.P. Koonce M. Schliwa Bidirectional organelle transport can occur in cell processes that contain single microtubules J. Cell Biol. 100 1985 322 326
149. M.P. Koonce P.M. Grissom J.R. McIntosh Dynein from Dictyostelium: Primary structure comparisons between a cytoplasmic motor enzyme and flagellar dynein J. Cell Biol. 119 1992 1597 1604
150. J. Kumar H. Yu M.P. Sheetz Kinectin, an essential anchor for kinesin-driven vesicle motility Science 267 1995 1834 1837
151. S.C. Kuo M.P. Sheetz Force of single kinesin molecules measured with optical tweezers Science 260 1993 232 234
152. R. Kuriyama G.G. Borisy Microtubule-nucleating activity of centrosomes in Chinese hamster ovary cells is independent of the centriole cycle but coupled to the mitotic cycle J. Cell Biol. 91 1981 822 826
153. S.A. Kuznetsov V.I. Gelfand Bovine brain kinesin is a microtubule-activated ATPase Proc. Natl. Acad. Sci. U.S.A. 83 1986 8530 8534
154. S.A. Kuznetsov Y.A. Vaisberg S.W. Rothwell D.B. Murphy V.I. Gelfand Isolation of a 45-kDa fragment from the kinesin heavy chain with enhanced ATPase and microtubule-binding activity J. Biol. Chem. 264 1989 589 595
155. S.A. Kuznetsov G.M. Langford D.G. Weiss Actin-dependent organelle movement in squid axoplasm Nature (London) 356 1992 722 725
156. M.L. Lacey L.T. Haimo Cytoplasmic dynein is a vesicle protein J. Biol. Chem. 267 1992 4793 4798
157. M.L. Lacey L.T. Haimo Cytoplasmic dynein binds to phospholipid vesicles Cell Motil. Cytoskel. 28 1994 205 212
158. Lazzaro, M., and Haimo, L. T. (1996). In preparation.
159. G. Lee S.L. Rook Expression of tau protein in non-neuronal cells: Microtubule binding and stabilization J. Cell Sci. 102 1992 227 237
160. K.-D. Lee P.J. Hollenbeck Phosphorylation of kinesin in vivo correlates with organelle association and neurite outgrowth J. Biol. Chem. 270 1995 5600 5605
161. A. Lee-Eiford R.A. Ow I.R. Gibbons Specific cleavage of dynein heavy chains by ultraviolet irradiation in the presence of ATP and vanadate J. Biol. Chem. 261 1986 2337 2342
162. J.P. Lees-Miller D.M. Helfman T.A. Schroer A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility Nature (London) 359 1992 244 246
163. P.L. Leopold A.W. McDowall K.K. Pfister G.S. Bloom S.T. Brady Association of kinesin with characterized membrane-bounded organelles Cell Motil. Cytoskel. 23 1992 19 33
164. S.A. Lewis D. Wang N.J. Cowan Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein Science 242 1988 936 939
165. S.A. Lewis I.E. Ivanov G.-H. Lee N.J. Cowan Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau Nature (London) 342 1989 498 505
166. M. Li M. McGrail M. Serr T.S. Hays Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte J. Cell Biol. 126 1994 1475 1494
167. Y.-Y. Li E. Yeh T. Hays K. Bloom Disruption of mitotic spindle orientation in a yeast dynein mutant Proc. Natl. Acad. Sci. U.S.A. 90 1993 10096 10100
168. H. Liao G. Li T.J. Yen Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein Science 265 1994 394 398
169. A. Lieuvin Labbé J. M. Dorée D. Job Intrinsic microtubule stability in interphase cells J. Cell Biol. 124 1994 985 996
170. S.H. Lillie S.S. Brown Suppression of a myosin defect by a kinesin-related gene Nature (London) 356 1992 358 361
171. S.H. Lillie S.S. Brown Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smylp, to the same regions of polarized growth in Saccharomyces cerevisiae. J. Cell Biol. 125 1994 825 842
172. S.X.H. Lin C.A. Collins Immunolocalization of cytoplasmic dynein to lysosomes in cultured cells J. Cell Sci. 101 1992 125 137
173. S.X.H. Lin C.A. Collins Regulation of the intracellular distribution of cytoplasmic dynein by serum factors and calcium J. Cell Sci. 105 1993 579 588
174. S.X.H. Lin K.L. Ferro C.A. Collins Cytoplasmic dynein undergoes intracellular redistribution concomitant with phosphorylation of the heavy chain in response to serum starvation and okadaic acid J. Cell Biol. 127 1994 1009 1019
175. G. Lindwall R.D. Cole Phosphorylation affects the ability of tau protein to promote microtubule assembly J. Biol. Chem. 259 1984 5301 5305
176. J. Lippincott-Schwartz N.B. Cole A. Marotta P.A. Conrad G.S. Bloom Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic J. Cell Biol. 128 1995 293 306
177. V.A. Lombillo C. Nislow T.J. Yen V.I. Gelfand J.R. McIntosh Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro J. Cell Biol. 128 1995 107 115
178. L.A. Lopez M.P. Sheetz Steric inhibition of cytoplasmic dynein and kinesin motility by MAP2 Cell Motil. Cytoskel. 24 1993 1 16
179. R.J. Lye M.E. Porter J.M. Scholey J.R. McIntosh Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans. Cell (Cambridge, Mass.) 51 1987 309 318
180. R.J. Lye C.M. Pfarr M.E. Porter Cytoplasmic dynein and microtubule translocators F.D. Warner J.R. McIntosh “Cell Movements” 2 1989 Alan R. Liss New York 141 154
181. R.W. Lymn E.W. Taylor Mechanism of adenosine triphosphate hydrolysis by actomyosin Biochemistry 10 1971 4617 4624
182. T.J. Lynch J.D. Taylor T.T. Tchen Regulation of pigment organelle translocation. I: Phosphorylation of the organelle associated protein p57 J. Biol. Chem. 261 1986 4204 4211
183. T.J. Lynch B.-Y. Wu J.D. Taylor T.T. Tchen Regulation of pigment organelle translocation. II: Participation of a cAMP dependent protein kinase J. Biol. Chem. 261 1986 4212 4216
184. H.J.G. Matthies R.J. Miller H.C. Palfrey Calmodulin binding to and cAMP-dependent phosphorylation of kinesin light chains modulate kinesin ATPase activity J. Biol. Chem. 268 1993 11176 11187
185. H.B. McDonald L.S.B. Goldstein Identification and characterization of a gene encoding a kinesin-like protein in Drosophila. Cell (Cambridge, Mass.) 61 1990 991 1000
186. H.B. McDonald R.J. Stewart L.S.B. Goldstein The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor Cell (Cambridge, Mass.) 63 1990 1159 1165
187. J.M. McIlvain J.K. Burkhardt S. Hamm-Alvarez Y. Argon M.P. Sheetz Regulation of kinesin activity by phosphorylation of kinesin-associated proteins J. Biol. Chem. 269 1994 19176 19182
188. F.J. McNally R.D. Vale Identification of katinin, an ATPase that severs and disassembles stable microtubules Cell (Cambridge, Mass.) 75 1993 419 429
189. P.B. Meluh M.D. Rose KAR3, a kinesin-related gene required for nuclear fusion Cell (Cambridge, Mass.) 60 1990 1029 1041
190. K. Middleton J. Carbon KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore Proc. Natl. Acad. Sci. U.S.A. 91 1994 7212 7216
191. A. Mikami B.M. Paschal M. Mazumdar R.B. Vallee Molecular cloning of the retrograde transport motor cytoplasmic dynein (MAP1C) Neuron 10 1993 787 796
192. R.H. Miller R.J. Lasek M.J. Katz Preferred microtubules for vesicle transport in lobster axons Science 235 1987 220 222
193. Y. Mimori T. Miki-Noumura Extrusion of rotating microtubules on the dynein-track from a microtubule-dynein-γ-complex Cell Motil. Cytoskel. 30 1995 17 25
194. D.R. Mitchell Cell and molecular biology of flagellar dyneins Int. Rev. Cytol. 155 1994 141 180
195. D.R. Mitchell K.S. Brown Sequence analysis of the Chlamydomonas alpha and beta dynein heavy chain genes J. Cell Sci. 107 1994 635 644
196. D.R. Mitchell Y. Kang Reversion analysis of dynein intermediate chain function J. Cell Biol. 113 1991 835 842
197. D.R. Mitchell J.L. Rosenbaun A motile Chlamydomonas flagellar mutant that lacks outer dynein arms J. Cell Biol. 100 1985 1228 1234
198. T. Mitchison M. Kirschner Dynamic instability of microtubule growth Nature (London) 312 1984 232 237
199. T. Mitchison M. Kirschner Microtubule assembly nucleated by isolated centrosomes Nature (London) 312 1984 237 242
200. M. Mooseker A multitude of myosins Curr. Biol. 3 1993 245 248
201. A.G. Moss J.-L. Gatti G.B. Witman The motile β/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond J. Cell Biol. 118 1992 1177 1188
202. L. Muhua T.S. Karpova J.A. Cooper A yeast actin-related protein homologus to that in vertebarate dynactin complex is important for spindle orientation and nuclear migration Cell (Cambridge, Mass.) 78 1994 669 679
203. A. Murakami Control of ciliary beat frequency in the gill of Mytilus -I. Activation of the lateral cilia by cyclic AMP Comp. Biochem. Physiol. C 86C 1987 273 279
204. H. Murofushi A. Ikai K. Okuhara S. Kotani H. Aizawa K. Kamakura H. Sakai Purification and characterization of kinesin from bovine adrenal medulla J. Biol. Chem. 263 1988 12744 12750
205. D.B. Murphy L.G. Tilney The role of microtubules in the movement of pigment granules in teleost melanophores J. Cell Biol. 61 1974 757 779
206. + kinesin-like gene and extracentromeric sequences are required for transmission of a Drosophila minichromosome Cell Cambridge, Mass.) 81 1995 139 148
207. M. Nangaku R. Sato-Yoshitake Y. Okada Y. Noda R. Takemura H. Yamazaki N. Hirokawa KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria Cell (Cambridge, Mass.) 79 1994 1209 1220
208. F. Navone J. Niclas N. Hom-Booher L. Sparks H.D. Bernstein G. McCaffrey R.D. Vale Cloning and expression of a human kinesin heavy chain gene: Interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells J. Cell Biol. 117 1992 1263 1275
209. B.W. Neighbors R.C. Williams Jr. J.R. McIntosh Localization of kinesin in cultured cells J. Cell Biol. 106 1988 1193 1204
210. C. Nislow V.A. Lombillo R. Kuriyama J.R. McIntosh A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles Nature (London) 359 1992 543 547
211. M. Noble S.A. Lewis N.J. Cowan The microtubule binding domain of microtubule-associated protein MAP1B contains a repeated sequence motif unrelated to that of MAP2 and tau J. Cell Biol. 109 1989 3367 3376
212. Y. Noda R. Sato-Yoshitake S. Kondo M. Nangaku N. Hirokawa KIF2 is a new microtubule-based anterograde motor that transport membranous organelles distinct from those carried by kinesin heavy chain or KIP3A/B J. Cell Biol. 129 1995 157 167
213. B.R. Oakley C.E. Oakley Y. Yoon M.K. Jung γ-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans. Cell (Cambridge, Mass.) 61 1990 1289 1301
214. C.E. Oakley B.R. Oakley Identification of γ tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans. Nature (London) 338 1989 662 664
215. M.J. O'Connell P.B. Meluh M.D. Rose N.R. Morris Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a KAR3-related kinesin-like protein in Aspergillus nidulans. J. Cell Biol. 120 1993 153 162
216. K. Ogawa Four ATP-binding sites in the midregion of the beta heavy chain of dynein Nature (London) 352 1991 643 645
217. C.K. Omoto K.A. Johnson Activation of the dynein adenosine-triphosphatase by microtubules Biochemistry 25 1986 419 427
218. C.K. Omoto C. Kung The pair of central tubules rotates during ciliary beat in Paramecium. Nature (London) 279 1979 532 534
219. C.K. Omoto G.W.B. Witman Functionally significant central-pair rotation in a primitive eukaryotic flagellum Nature (London) 290 1981 708 710
220. cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics J. Cell Biol. 128 1995 849 862
221. L.K. Opresko C.J. Brokaw cAMP-dependent phosphorylation associated with activation of motility of Ciona sperm flagella Gamete Res. 8 1983 201 218
222. R.E. Palazzo T.J. Lynch J.D. Taylor T.T. Tchen cAMP-independent and cAMP-dependent protein phosphorylations by isolated goldfish xanthophore cytoskeletons: Evidence for the association of cytoskeleton with a carotenoid droplet protein Cell Motil. Cytoskel. 13 1989 21 29
223. B.M. Paschal R.B. Vallee Retrograde transport by the microtubule-associated protein MAP 1C Nature (London) 330 1987 181 183
224. B.M. Paschal H.S. Shpetner R.B. Vallee MAP1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties J. Cell Biol. 105 1987 1273 1282
225. B.M. Paschal S.M. King A.G. Moss C.A. Collins R.B. Vallee G.B. Witman Isolated flagellar outer arm dynein translocates brain microtubules in vitro Nature (London) 330 1987 672 674
226. B.M. Paschal R.A. Obar R.B. Vallee Interaction of brain cytoplasmic dynein and MAP2 with a common sequence at the C terminus of tubulin Nature (London) 342 1989 569 572
227. B.M. Paschal A. Mikami K.K. Pfister R.B. Vallee Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function J. Cell Biol. 118 1992 1133 1143
228. glued and a novel actin J. Biol. Chem. 268 1993 15318 15323
229. C.M. Pfarr M. Coue P.M. Grissom T.S. Hays M.E. Porter J.R. McIntosh Cytoplasmic dynein is localized to kinetochores during mitosis Nature (London) 345 1990 263 265
230. K.K. Pfister G.B. Witman Subfractionation of Chlamydomonas 18S dynein into two unique subunits containing ATPase activity J. Biol. Chem. 259 1984 12072 12080
231. K.K. Pfister B.E. Haley G.B. Witman The photoaffinity probe 8-azidoadenosine 5′-triphosphate selectively labels the heavy chain of Chlamydomonas 12 S dynein J. Biol. Chem. 259 1984 8499 8504
232. K.K. Pfister B.E. Haley G.B. Witman Labeling of Chlamydomonas 18S dynein polypeptides by 8-azidoadenosine 5′-triphosphate, a photoaffinity analog of ATP J. Biol. Chem. 260 1985 12844 12850
233. K.K. Pfister M.C. Wagner D.L. Stenoien S.T. Brady G.S. Bloom Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells J. Cell Biol. 108 1989 1453 1464
234. G. Piperno M.T. Fuller Monoclonal antibodies specific for an acetylated form of α-tubulin recognize the antigen in cilia and flagella from a variety of organisms J. Cell Biol. 101 1985 2085 2094
235. G. Piperno Z. Ramanis The proximal portion of Chlamydomonas flagella contains a distinct set of inner dynein arms J. Cell Biol. 112 1991 701 709
236. G. Piperno Z. Ramanis E.F. Smith W.S. Sale Three distinct inner dynein arms in Chlamydomonas flagella: Molecular composition and location in the axoneme J. Cell Biol. 110 1990 379 389
237. G. Piperno K. Mead W. Shestak The inner dynein arms 12 interact with a “dynein regulatory complex” in Chlamydomonas flagella J. Cell Biol. 118 1992 1455 1463
238. G. Piperno K. Mead M. LeDizet A. Moscatelli Mutations in the “dynein regulatory complex” alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes J. Cell Biol. 125 1994 1109 1117
239. M. Plamann P.F. Minke J.H. Tinsley K.S. Bruno Cytoplasmic dynein and actin-related Arpl are required for normal nuclear distribution in filamentous fungi J. Cell Biol. 127 1994 139 149
240. M.E. Porter J. Power S.K. Dutcher Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms J. Cell Biol. 118 1992 1163 1176
241. pf1 locus of Chlamydomonas reinhardtii identify a regulatory domain in the beta dynein heavy chain J. Cell Biol. 126 1994 1495 1508
242. K. Rasmusson M. Serr J. Gepner I. Gibbons T.S. Hays A family of dynein genes in Drosophila melanogaster. Mol. Biol. Cell 5 1994 45 55
243. V.I. Rodionov F.K. Gyoeva A.S. Ksashina S.A. Kuznetsov V.I. Gelfand Microtubule-associated proteins and microtubule-based translocators have different binding sites on tubulin molecules J. Biol. Chem. 265 1990 5702 5707
244. V.I. Rodionov F.K. Gyoeva V.I. Gelfand Kinesin is responsible for centrifugal movement of pigment granules in melanophores Proc. Natl. Acad. Sci. U.S.A. 88 1991 4956 4960
245. L. Romberg R.D. Vale Chemomechanical cycle of kinesin differs from that of myosin Nature (London) 361 1993 168 170
246. D.M. Roof P.B. Meluh M.D. Rose Kinesin-related proteins required for assembly of the mitotic spindle J. Cell Biol. 118 1992 95 108
247. M.M. Rozdzial L.T. Haimo Reactivated melanophore motility: differential regulation and nucleotide requirements of bidirectional pigment granule transport J. Cell Biol. 103 1986 2755 2764
248. M.M. Rozdzial L.T. Haimo Bidirectional pigment granule movements of melanophores are regulated by protein phosphorylation and dephosphorylation Cell (Cambridge, Mass.) 47 1986 1061 1070
249. K.M. Ruppel J.A. Spudich Myosin motor function: Structural and mutagenic approaches Curr. Opin. Cell Biol. 7 1995 89 93
250. H. Sakakibara D.R. Mitchell R. Kamiya A Chlamydomonas outer dynein mutant missing the a heavy chain J. Cell Biol. 113 1991 615 622
251. H. Sakakibara S. Takada S.M. King G.B. Witman R. Kamiya A Chlamydomonas outer arm dynein mutant with a truncated β heavy chain J. Cell Biol. 122 1993 653 661
252. 2+-induced asymmetry of active microtubule sliding in sea urchin sperm tails J. Cell Biol. 102 1986 2042 2052
253. W.S. Sale L.A. Fox Isolated β-heavy chain subunit of dynein translocates microtubules in vitro. J. Cell Biol. 107 1988 1793 1797
254. W.S. Sale P. Satir Direction of active sliding of microtubules in Tetrahymena cilia Proc. Natl. Acad. Sci. U.S.A. 74 1977 2045 2049
255. W.S. Sale U.W. Goodenough J.E. Heuser The substructure of isolated and in situ outer dynein arms of sea urchin sperm flagella J. Cell Biol. 101 1985 1400 1412
256. E.D. Salmon R.J. Leslie W.M. Saxton M.L. Karow J.R. McIntosh Spindle microtubule dynamics in sea urchin embryos. Analysis using fluorescence-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching J. Cell Biol. 99 1984 2165 2174
257. R. Sato-Yoshitake H. Yorifuji M. Inagaki N. Hirokawa The phosphorylation of kinesin regulates its binding to synaptic vesicles J. Biol. Chem. 267 1992 23930 23936
258. W.S. Saunders M.A. Hoyt Kinesin-related proteins required for structural integrity of the mitotic spindle Cell (Cambridge, Mass.) 70 1992 451 458
259. W.S. Saunders D. Koshland D. Eschel I.R. Gibbons M.A. Hoyt Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation J. Cell Biol. 128 1995 617 624
260. K.E. Sawin K.L. Le Guellec M. Philippe T.J. Mitchison Mitotic spindle organization by a plus end-directed microtubule motor Nature (London) 359 1992 540 543
261. W.M. Saxton D.L. Stemple R.J. Leslie E.D. Salmon M. Zavortink J.R. McIntosh Tubulin dynamics in cultured mammalian cells J. Cell Biol. 99 1984 2175 2186
262. D.A. Schafer S.R. Gill J.A. Cooper J.E. Heuser T.A. Schroer Ultrastructural analysis of the dynactin complex: An actin-related protein is a component of a filament that resembles F-actin J. Cell Biol. 126 1994 403 412
263. F. Schmitz K.T. Wallis M. Rho D. Drenckhahn D.B. Murphy Intracellular distribution of kinesin in chromaffin cells Eur. J. Cell Biol. 63 1994 77 83
264. B.J. Schnapp T.S. Reese Dynein is the motor for retrograde axonal transport of organelles Proc. Natl. Acad. Sci. U.S.A. 86 1989 1548 1552
265. B.J. Schnapp R.D. Vale M.P. Sheetz T.S. Reese Single microtubules from squid axoplasm support bidirectional movement of organelles Cell (Cambridge, Mass.) 40 1985 455 462
266. J.M. Scholey J. Heuser J.T. Yang L.S.B. Goldstein Identification of globular mechanochemical heads of kinesin Nature (London) 38 1989 335 357
267. T.A. Schroer Structure, function and regulation of cytoplasmic dynein Curr. Opin. Cell Biol. 6 1994 69 73
268. T.A. Schroer M.P. Sheetz Two activators of microtubule-based vesicle transport J. Cell Biol. 115 1991 1309 1318
269. T.A. Schroer B.J. Schnapp T.S. Reese M.P. Sheetz The role of kinesin and other soluble factors in organelle movements along microtubules J. Cell Biol. 107 1988 1785 1792
270. T.A. Schroer E.R. Steuer M.P. Sheetz Cytoplasmic dynein is a minus end-directed motor for membranous organelles Cell (Cambridge, Mass.) 56 1989 937 946
271. E. Schulze M. Kirschner Microtubule dynamics in interphase cells J. Cell Biol. 102 1986 1020 1031
272. Y. Sekine Y. Okada Y. Noda S. Kondo H. Aizawa R. Takemura N. Hirokawa A novel microtubule-based motor protein (KIF4) for organelle transport, whose expression is regulated developmentally J. Cell Biol. 127 1994 187 201
273. N. Shiina T. Moriguchi K. Ohta Y. Gotoh E. Nishida Regulation of a major microtubule associated protein by MPF and MAP kinase EMBO J. 11 1992 3977 3984
274. N. Shiina Y. Gotoh E. Nishida A novel homo-oligomeric protein responsible for an MPF-dependent microtubule-severing activity EMBO J. 11 1992 4723 4731
275. N. Shiina Y. Gotoh N. Kubomura A. Iwamatsu E. Nishida Microtubule severing by elongation factor 1α Science 266 1994 282 285
276. T. Shimizu Y.Y. Toyoshima M. Edamatsu R.D. Vale Comparison of the motile and enzymatic properties of two microtubule minus-end-directed motors, ncd and cytoplasmic dynein Biochemistry 34 1995 1575 1582
277. H.S. Shpetner B.M. Paschal R.B. Vallee Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP1C) J. Cell Biol. 107 1988 1001 1009
278. D.A. Skoufias J.M. Scholey Cytoplasmic microtubule-based motor proteins Curr. Opin. Cell Biol. 5 1993 95 104
279. D.A. Skoufias D.G. Cole K.P. Wedaman J.M. Scholey The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding J. Biol. Chem. 269 1994 1477 1485
280. R.D. Sloboda S.A. Rudolph J.L. Rosenbaum P. Greengard Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein Proc. Natl. Acad. Sci. U.S.A. 72 1975 177 181
281. E.F. Smith W.S. Sale Microtubule binding and translocation by inner dynein arm subtype I1 Cell Motil. Cytoskel. 18 1991 258 268
282. E.F. Smith W.S. Sale Regulation of dynein-driven microtubule sliding by the radial spokes in flagella Science 257 1992 1557 1559
283. E.F. Smith W.S. Sale Structural and functional reconstitution of inner dynein arms in Chlamydomonas flagellar axonemes J. Cell Biol. 117 1992 573 581
284. E. Sontag V. Nunbhakdi-Craig G.S. Bloom Mumby M.C. A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle J. Cell Biol. 128 1995 1131 1144
285. T. Stearns M. Kirschner In vitro reconstitution of centrosome assembly and function: The central role of gamma-tubulin Cell (Cambridge, Mass.) 76 1994 623 637
286. T. Stearns L. Evans M. Kirschner γ-tubulin is a highly conserved component of the centrosome Cell (Cambridge, Mass.) 65 1991 825 836
287. R.E. Stephens G. Prior Dynein from serotonin-activated cilia and flagella: Extraction characteristics and distinct sites of cAMP-dependent protein phosphorylation J. Cell Sci. 103 1992 999 1012
288. E.R. Steuer L. Wordeman T.A. Schroer M.P. Sheetz Localization of cytoplasmic dynein to mitotic spindles and kinetochores Nature (London) 345 1990 266 268
289. R.J. Stewart J.P. Thaler L.S. Goldstein Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein Proc. Natl. Acad. Sci. U.S.A. 90 1993 5209 5213
290. E.W. Stommel R.E. Stephens Calcium-dependent phosphatidylinositol phosphorylation in lamellibranch gill lateral cilia J. Comp. Physiol. A 157A 1985 451 459
291. K.E. Summers I.R. Gibbons Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm Proc. Natl. Acad. Sci. U.S.A. 68 1971 3092 3096
292. K. Svoboda S.M. Block Force and velocity measured for single kinesin molecules Cell (Cambridge, Mass.) 11 1994 773 784
293. K. Svoboda C.F. Schmidt B.J. Schnapp S.M. Block Direct observation of kinesin stepping by optical trapping interferometry Nature (London) 365 1993 721 727
294. A. Swaroop M. Swaroop A. Garen Sequence analysis of the complete cDNA and encoded polypeptide for the glued gene of Drosophila melanogaster. Proc. Natl. Acad. Sci. U.S.A. 84 1987 6501 6505
295. R. Takemura S. Okabe T. Umeyama Y. Kanai N.J. Cowan N. Hirokawa Increased microtubule stability and alpha tubulin acetylation in cells transfected with microtubule-associated proteins, MAP1B, MAP2 or tau J. Cell Sci. 103 1992 953 964
296. J.L. Tan S. Ravid J.A. Spudich Control of nonmuscle myosins by phosphorylation Annu. Rev. Biochem. 61 1992 721 759
297. J.S. Tash M. Krinks J. Patel R.L. Means C.B. Klee A.R. Means Identification, characterization, and functional correlation of calmodulin-dependent protein phosphatase in sperm J. Cell Biol. 106 1988 1625 1633
298. C.D. Thaler L.T. Haimo Regulation of organelle transport in melanophores by calcineurin J. Cell Biol. 111 1990 1939 1948
299. 2+ and cAMP levels Cell Motil. Cytoskel. 22 1992 175 184
300. W.E. Theurkauf R.B. Vallee Molecular characterization of the cAMP-dependent protein kinase bound to microtubule-associated protein 2 J. Biol. Chem. 257 1982 3284 3290
301. M.A. Titus Multitasking with myosin Trends Genet. 9 1993 187 188
302. I. Toyoshima H. Yu E.R. Steuer M.P. Sheetz Kinectin, a major kinesin binding protein J. Cell Biol. 118 1992 1121 1131
303. S. Tsuyama G.T. Bramblett K.P. Huang M. Flavin Calcium/phospholipid-dependent kinase recognizes sites in microtubule-associated protein 2 which are phosphory-lated in living brain and are not accessible to other kinases J. Biol. Chem. 261 1986 4110 4116
304. R. Urrutia M.A. McNiven J.P. Albanesi D.B. Murphy B. Kachar Purified kinesin promotes vesicle motility and induces active sliding between microtubules in vitro. Proc. Natl. Acad. Sci. U.S.A. 88 1991 6701 6705
305. T.Q. Uyeda H.M. Warrick S.J. Kron J.A. Spudich Quantized velocities at low myosin densities in an in vitro motility assay Nature (London) 352 1991 307 311
306. E.A. Vaisberg M.P. Koonce J.R. McIntosh Cytoplasmic dynein plays a role in mammalian mitotic spindle formation J. Cell Biol. 123 1993 849 858
307. R.D. Vale Severing of stable microtubules by a mitotically-activated protein in Xenopus egg extracts Cell (Cambridge, Mass.) 64 1991 827 839
308. R.D. Vale H. Hotani Formation of membrane networks in vitro by kinesindriven microtubule movement J. Cell Biol. 107 1988 2233 2241
309. R.D. Vale Y.Y. Toyoshima Rotation and translocation of microtubules in vitro induced by dyneins from Tetrahymena cilia Cell (Cambridge, Mass.) 52 1988 459 469
310. R.D. Vale B.J. Schnapp T.S. Reese M.P. Sheetz Organelle, bead, andmicrotubule translocations promoted by soluble factors from the squid giant axon Cell (Cambridge, Mass.) 40 1985 559 569
311. R.D. Vale T.S. Reese M.P. Sheetz Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility Cell (Cambridge, Mass.) 42 1985 39 50
312. R.D. Vale B.J. Schnapp T. Mitchison E. Steuer T.S. Reese M.P. Sheetz Different axoplasmic proteins generate movements in opposite directions along microtubules in vitro Cell (Cambridge, Mass.) 43 1985 623 632
313. R.D. Vale F. Malik D. Brown Directional instability of microtubule transport in the presence of kinesin and dynein, two opposite polarity motor proteins J. Cell Biol. 119 1992 1589 1596
314. M.L. Vallano J.R. Goldering T.M. Buckholz R.E. Larson R.J. Delorenzo Separation of endogenous calmodulin and cAMP dependent kinases from microtubule preparations Proc. Natl. Acad. Sci. U.S.A. 83 1985 3203 3208
315. R. Vallee Molecular analysis of the microtubule motor dynein Proc. Natl. Acad. Sci. U.S.A. 90 1993 8769 8772
316. R.B. Vallee J.S. Wall B.M. Paschal H.S. Shpetner Microtubule-associated protein 1C from brain is a two-headed cytosolic dynein Nature (London) 332 1988 561 563
317. F. Verde Labbé J.C. M. Dorée E. Karsenti Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs Nature (London) 343 1990 233 238
318. R. Verde M. Dogterom E. Stelzer E. Karsenti S. Leibler Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts J. Cell Biol. 118 1992 1097 1108
319. I. Vernos J. Raats T. Hirano J. Heasman E. Karsenti C. Wylie Xklpl, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning Cell (Cambridge, Mass.) 81 1995 117 127
320. A. von Massow E.M. Mandelkow E. Mandelkow Interaction between kinesin, microtubules and microtubule-associated protein 2 Cell Motil. Cytoskel. 14 1989 562 571
321. R.A. Walker M.P. Sheetz Cytoplasmic microtubule-associated motors Annu. Rev. Biochem. 62 1993 429 451
322. R.A. Walker E.D. Salmon S.A. Endow The Drosophila claret segregation protein is a minus-end directed motor molecule Nature (London) 347 1990 780 782
323. Z. Walther M. Vashishtha J.L. Hall The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein J. Cell Biol. 126 1994 175 188
324. S.-Z. Wang R. Adler Chromokinesin: A DNA-binding, kinesin-like nuclear protein J. Cell Biol. 128 1995 761 768
325. D.R. Webster J. Wehland K. Weber G.G. Borisy Detyrosination of α-tubulin does not stabilize microtubules in vivo. J. Cell Biol. 111 1990 113 122
326. A. Weiss D.C. Mayer L.A. Leinwand Diversity of myosin-based motility: multiple genes and functions Soc. Gen. Physiol. Ser. 49 1994 159 171
327. B. Weisshaar T. Doll A. Matus Reorganization of the microtubular cytoskeleton by embryonic microtubule-associated proteins 2 (M AP2c) Development (Cambridge, Mass.) 116 1992 1151 1161
328. C.G. Wilkerson S.M. King G.B. Witman Molecular analysis of the γ heavy chain of Chlamydomonas flagellar outer-arm dynein J. Cell Sci. 107 1994 497 506
329. G.B. Witman Axonemal dyneins Curr. Opin. Cell Biol. 4 1992 74 79
330. G.B. Witman J. Plummer G. Sander Chlamydomonas flagellar mutants lacking radial spokes and central tubules J. Cell Biol. 76 1978 729 747
331. L. Wordeman T.J. Mitchison Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centro-somes during mitosis J. Cell Biol. 128 1995 95 105
332. L. Wordeman E.R. Steuer M.P. Sheetz T.J. Mitchison Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes J. Cell Biol. 114 1991 285 294
333. X. Xiang S.M. Beckwith N.R. Morris Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans. Proc. Natl. Acad. Sci. U.S.A. 91 1994 2100 2104
334. J.T. Yang R.A. Laymon L.S.B. Goldstein A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses Cell (Cambridge, Mass.) 56 1989 879 889
335. J.T. Yang W.M. Saxton R.J. Stewart E.C. Raff L.S.B. Goldstein Evidence that the head of kinesin is sufficient for force generation and motility in vitro. Science 249 1990 42 47
336. T.J. Yen G. Li B.R. Schaar I. Szilak D.W. Cleveland CENP-E is a putative kinetochore motor that accumulates just before mitosis Nature (London) 359 1992 536 539
337. H. Yu I. Toyoshima E.R. Steuer M.P. Sheetz Kinesin and cytoplasmic dynein binding to brain microsomes J. Biol. Chem. 267 1992 20457 20464
338. P. Zhang A. Knowles L.S.B. Goldstein R.S. Hawley A kinesin-like protein required for distributive chromosome segregation in Drosophila. Cell (Cambridge, Mass.) 62 1990 1053 1062
339. Z. Zhang Y. Tanaka S. Nonaka H. Aizawa H. Kawasaki T. Nakata N. Hirokawa The primary structure of rat brain (cytoplasmic) dynein heavy chain, a cytoplasmic motor enzyme Proc. Natl. Acad. Sci. U.S.A. 90 1993 7928 7932
340. Y. Zheng M.K. Jung B.R. Oakley γ tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centromere Cell (Cambridge, Mass.) 65 1991 817 823