Rapid freeze-quench ENDOR of the radical X intermediate of Escherichia coli ribonucleotide reductase using 17O2, H217O, and 2H2O

Journal of the American Chemical Society

Volumn 118, Issue 1, 1996, Pages 281-282

  Burdi, Doug ^a   Sturgeon, Bradley E ^b   Tong, Wing H ^a   Stubbe, Joanne ^a   Hoffman, Brian M ^b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030020753     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja952651e     Document Type: Article

References (20)

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9. DeRose, V. J.; Hoffman, B. M. In Methods in Enzvmalogy; Sauer, K., Ed., Academic Press: New York, 1995; pp 555-589.
10. Q-band CW ENDOR spectra were collected as described previously (Werst, M. M.; Davoust, C. E.; Hoffman, B M J. Am. Chem. Soc. 1991, 113, 1533-1538), using bandwidth broadening (100 kHz) of the radio frequency as described ( Hoffman, B. M.; DeRose, V J.; Ong, J.-L.; Davoust, C. E. J. Magn, Reson., Ser. A 1994, 110, 52-57).
11. Q-band CW ENDOR spectra were collected as described previously (Werst, M. M.; Davoust, C. E.; Hoffman, B M J. Am. Chem. Soc. 1991, 113, 1533-1538), using bandwidth broadening (100 kHz) of the radio frequency as described ( Hoffman, B. M.; DeRose, V J.; Ong, J.-L.; Davoust, C. E. J. Magn, Reson., Ser. A 1994, 110, 52-57).
12. Wertz, J. E.; Bolton, J. R. Electron spin resonance, elementary theory and practical applications, 2nd ed.; Chapman and Hall: New York, 1986.
13. Efforts are under way to explore similarities of these signals with those of the hydroxo bridge and water of mixed-valence duron centers (DeRose, V. J.; Liu, K. E.; Kurtz, D. M., Jr.; Huffman, B. M.; Lippard, S. J. J. Am. Chem. Soc. 1993, 155, 6440-6441. DeRose, V. J.; Liu, K. E.; Lippard, S. J.; Hoffman, B. M. J. Am. Chem. Soc. 1996, 118, 121-134.
14. Efforts are under way to explore similarities of these signals with those of the hydroxo bridge and water of mixed-valence duron centers (DeRose, V. J.; Liu, K. E.; Kurtz, D. M., Jr.; Huffman, B. M.; Lippard, S. J. J. Am. Chem. Soc. 1993, 155, 6440-6441. DeRose, V. J.; Liu, K. E.; Lippard, S. J.; Hoffman, B. M. J. Am. Chem. Soc. 1996, 118, 121-134.
15. 17O.
16. Werst, M. M.; Kennedy, M. C.; Beinen, H.; Hoffman, B. M. Biochemistry 1990, 29, 10526-10532.
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19. Sturgeon, B. E.; Burdi, D.; Stubbe, J.; Hoffman, B. M., unpublished results.
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