Improved kinetic analysis of cytosolic free calcium in pressure-sensitive neuronal cells by fixed-time flow cytometry

Cytometry

Volumn 23, Issue 1, 1996, Pages 82-89

  Tárnok, Attila ^a,b  

^a UNIVERSITY OF HAMBURG   (Germany)

^b UNIVERSITY HOSPITAL LEIPZIG   (Germany)

Author keywords

bradykinin; cytosolic free calcium; fixed time flow cytometry; neuronal cell lines; NH15 CA2; on line ligand injection; pressure induced Ca2+ (i) response

Indexed keywords

BRADYKININ; CALCIUM;

ARTICLE; CONTROLLED STUDY; CYTOSOL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; KINETICS; NERVE CELL; PRIORITY JOURNAL; TUMOR CELL LINE;

3T3 CELLS; ANIMALS; BRADYKININ; CALCIUM; CALCIUM SIGNALING; CELL LINE, TUMOR; CULTURE MEDIA; CYTOSOL; FLOW CYTOMETRY; KINETICS; MICE; MICROSCOPY, CONFOCAL; NEURONS; PRESSURE; REACTION TIME; TIME FACTORS;

EID: 0030020586     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19960101)23:1<82::AID-CYTO13>3.3.CO;2-1     Document Type: Article

References (23)

1. Beisker W: A new combined integral-light and slit-scan data analysis system (DAS) for flow cytometry. Comput Methods Prog Biomed 42:15-26, 1994.
2. Boltz RCD, Sirotina A, Blake T, Kath G, Uhrig B, McKell J, Quinn C: A disposable-chamber temperature-regulation system for the study of intracellular calcium levels in single living T cells using fluorescence digital-imaging microscopy. Cytometry 17:128-134, 1994.
3. Dunne JF: Time window analysis and sorting. Cytometry 12:597-601, 1991.
4. Elsner J, Norgauer J, Dobos GJ, Emmendörfer A, Schöpf E, Kapp A, Roesler J: Flow cytometry reveals different lag times in rapid cytoplasmic calcium elevations in human neutrophils in response to N-Formyl peptide. J Cell Physiol 157:637-643, 1993.
5. Erxleben C, Ubl J, Kolb HA: Identifying and characterizing stretch-activated ion channels. In: Molecular Neurobiology. A Practical Approach, Chad J, Wheal H (eds). IRL Press, Oxford, 1991, pp 75-94.
6. i indicators with greatly improved fluorescence properties. J Biol Chem 260:3440-3450, 1985.
7. 2+ influx gated by inositol-3,4,5,6-tetrakisphosphate in NIH/3T3 fibroblasts. Biochem Biophys Res Commun 200:1300-1306, 1944.
8. Heumann R, Öcalan M, Kachel V, Hamprecht B: Clonal hybrid cell lines expressing cholinergic and adrenergic properties. Proc Natl Acad Sci USA 76:4674-4677, 1979.
9. Jones GD, Gear ARL: Subsecond calcium dynamics in ADP- and thrombin-stimulated platelets: A continuous-flow approach using indo-1. Blood 71:1539-1543, 1988.
10. Kao JPY, Haroorunian AT, Tsien RY: Photochemically generated calcium pulses and their detection by fluo-3. J Biol Chem 264:8179-8184, 1989.
11. Kelley KA: Sample station modification providing on-line reagent addition and reduced sample transit time for flow cytometers. Cytometry 10:796-800, 1989.
12. Lindberg W, Scampavia LD, Ruzicka J. Christian GD: Fast kinetic measurements and on-line dilution by flow injection cytometry. Cytometry 16:324-330, 1994.
13. Minta A, Kao JPY, Tsien RY: Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 264:8171-8178, 1989.
14. Omann GM, Coppersmith W, Finney DA, Sklar LA: A convenient on-line device for reagent addition, sample mixing, and temperature control of cell suspensions in flow cytometry. Cytometry 6:69-73, 1985.
15. 2+ of cultured human neuroblastoma cells stimulated with carbachol: Relevance to high pressure neurological syndrome Cell Calcium 15:117-121, 1994.
16. Rabinovitch PS, June CH: Intracellular ionized calcium, membrane potential, and pH. In: Flow Cytometry. A Practical Approach, Ormerod MG (ed). IRL Press, Oxford, 1990, pp 161-187.
17. Rabinovitch PS, June CH, Grossmann A, Ledbetter JA: Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. J Immunol 137:952-961, 1986.
18. 2+ activation in a neuroblastoma x glioma hybrid cell line. J Neurochem 62:890-897, 1994.
19. Schieren J, MacDermott A: Flow cytometric identification and purification of cells by ligand-induced changes in intracellular calcium. J Neurosci Methods 26:35-44, 1988.
20. 2+and suppression of inward currents in a neuronal hybrid cell line triggered by bradykinin. Brain Res 524: 219-224, 1990.
21. Szöllösi J. Feuerstein BG, Hyun WC, Das MK, Marton LJ: Attachment of A127 human glioblastoma cells affects calcium signalling: a comparison of image cytometry, flow cytometry, and spectrofluorometry. Cytometry 12:707-716, 1991.
22. i in bovine alveolar macrophages. Anal Cell Pathol 4:234, 1992.
23. Vandrope DH, Morris CE: Stretch activation of the aplysia S-channel. J Membr Biol 127:205-214, 1992.