Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V

Blood

Volumn 87, Issue 3, 1996, Pages 1179-1187

  Kuypers, Frans A ^a   Lewis, Rachel A ^b   Hua, Mandy ^b   Schott, Mary Ann ^b   Discher, Dennis ^b   Ernst, Joel D ^b   Lubin, Bertram H ^b  

^a CHILDREN S HOSPITAL OAKLAND RESEARCH INSTITUTE   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; MEMBRANE PHOSPHOLIPID; PHOSPHATIDYLSERINE;

ARTICLE; ERYTHROCYTE MEMBRANE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; MEMBRANE STRUCTURE; PRIORITY JOURNAL; SICKLE CELL ANEMIA;

EID: 0030020463     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood.v87.3.1179.bloodjournal8731179     Document Type: Article

References (32)

1. Bretscher M: Asymmetrical lipid bilayer structure for biological membranes. Nature 236:11, 1972
2. Bretscher M: Phosphatidylethanolamine: Differential labeling in intact cells and cell ghosts of human erythrocytes by a membrane impermeable reagent. J Mol Biol 71:523, 1972
3. Devaux PF: Static and dynamic lipid asymmetry in cell membranes. Biochemistry 30:1163, 1991
4. Etemadi AH: Membrane asymmetry. A survey and critical appraisal of the methodology. II. Methods for assessing the unequal distribution of lipids. Biochim Biophys Acta 604:423, 1980
5. Op den Kamp J: Lipid asymmetry in membranes. Annu Rev Biochem 48:47, 1979
6. Schroit AJ, Zwaal RFA: Transbilayer movement of phospholipids in red cell and platelet membranes. Biochim Biophys Acta 1071.313, 1991
7. Roelofsen B: Phospholipases as tools to study the localization of phospholipids in biological membranes: A critical review. J Toxicol 1:87, 1982
8. Zachowski A: Phospholipids in animal eukaryotic membranes: Transverse asymmetry and movement. Biochem 294:1, 1993
9. Geldwerth D, Kuypers FA, Bütikofer P, Allary M, Lubin BH, Devaux PF: Transbilayer mobility and distribution of red cell phospholipids during storage. J Clin Invest 92:308, 1993
10. 2+-induced phospholipid redistribution in the human erythrocyte membrane. J Biol Chem 269:6347, 1994
11. Kuypers FA, van den Berg JJM, Lubin BH: Phospholipid asymmetry and diffusion in the membrane of normal and sickle erythrocytes, in Onishi S. (ed): Membrane Linked Diseases. Boca Raton, FL, CRC, 1994, p 21
12. Blumenfeld N, Zachowski A, Galacteros F, Beuzard Y, Devaux P: Transmembrane mobility of phospholipids in sickle erythrocytes: Effect of deoxygenation on diffusion and asymmetry. Blood 77:849, 1991
13. Tait JF, Gibson D: Measurement of membrane phospholipid asymmetry in normal and sickle cell erythrocytes by means of annexin binding. J Lab Clin Med 123:741, 1994
14. Ernst JD: Epitope mapping of annexin I. Antibodies that compete with phospholipids and calcium recognize amino acids 42-99. Biochem 289:539, 1993
15. Raynal P, Pollard HB: Annexins: The problem of assessing the biological role for a gene family of multifunctional calcium-and phospholipid-binding proteins. Biochim Biophys Acta 1197:63, 1994
16. Dachary-Prigent J, Freyssinet JM, Pasquet JM, Carron JC, Nurden AT: Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: A flow cytometry study showing a role for free sulthydryl groups. Blood 81:2554, 1993
17. Koopman G, Reutelingsperger CPM, Kuijten GAM, Keehnen RMJ, Pals ST, van Oers MHJ: Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415, 1994
18. Comfurius P, Senden JM, Tilly RH, Schroit AJ, Bevers EM, Zwaal RF: Loss of membrane phospholipid asymmetry in platelets and red cells may be associated with calcium-induced shedding of plasma membrane and inhibition of aminophospholipid translocase. Biochim Biophys Acta 1026:153, 1990
19. Baldwin JM, O'Reilly R, Whitney M, Lucy JA: Surface exposure of phosphatidylserine is associated with the swelling and osmotically-induced fusion of human erythrocytes in the presence of Ca2+. Biochim Biophys Acta 1028:14, 1990
20. Connor J, Gillum K, Schroit AJ: Maintenance of lipid asymmetry in red blood cells and ghosts: Effect of divalent cations and serum albumin on the transbilayer distribution of phosphatidylserine. Biochim Biophys Acta 1025:82, 1990
21. Williamson P, Kulick A, Zachowski A, Schlegel RA, Devaux PF: Ca2+ induces transbilayer redistribution of all major phospholipids in human erythrocytes. Biochemistry 31:6355, 1992
22. Butikofer P, Kuypers FA, Xu CM, Chiu DT, Lubin B: Enrichment of two glycosyl-phosphatidylinositol-anchored proteins, acetylcholinesterase and decay accelerating factor, in vesicles released from human red blood cells. Blood 74:1481, 1989
23. Wagner GM, Chiu DTY, Yee M, Lubin BH: Red cell vesiculation - A common membrane physiologic event. J Lab Clin Med 108:315, 1986
24. Ernst JD, Hoye E, Blackwood RA: Use of a novel strategy for the preparation and characterization of an antipeptide antibody capable of recognizing members of the annexin family. Biochem Biophys Res Commun 161:959, 1989
25. Haigler HT, Schlaepfer DD, Burgess WH: Characterization of lipocortin I and an immunologically unrelated 33 kDa protein as epidermal growth factor receptor/kinase substrates and phospholipase A2 inhibitors. J Biol Chem 262:6921, 1987
26. Ernst JD, Blackwood RA, Hoye E, Mok TL: Identification of a domain that mediates vesicle aggregation reveals functional diversity of annexin repeats J Biol Chem 266:6670, 1991
27. 2+ and phospholipid binding domains. Biochem Biophys Res Commun 200:867, 1994
28. Discher DE, Lenng A, Mohandas N, Evans EA: Molecular maps of red cell deformation: Hidden elasticity and in situ connectivity. Science 266:1032, 1994
29. Blackwood RA, Ernst JD: Characterization of Ca2+-dependent phospholipid binding, vesicle aggregation and membrane fusion by annexins. Biochem J 266:195, 1990
30. Butikofer P, Lin ZW, Chiu DT, Lubin B, Kuypers FA: Transbilayer distribution and mobility of phosphatidylinositol in human red blood cells. J Biol Chem 265:16035, 1990
31. Zachowski A, Favre E, Cribier S, Herve P, Devaux PF: Outside-inside translocation of aminophospholipids in the human erythrocyte membrane is mediated by a specific enzyme [published erratum appears in Biochemistry 25:7788, 1986]. Biochemistry 25:2585, 1986
32. Bitbol M, Fellmann P, Zachowski A, Devaux PF: Ion regulation of phosphatidylserine and phosphatidylethanolamine outside-inside translocation in human erythrocytes. Biochim Biophys Acta 904:268, 1987