Purification characterisation of the pyruvate decarboxylase from a haploid strain of saccharomyces cerevisiae

Biological Chemistry Hoppe-Seyler

Volumn 377, Issue 5, 1996, Pages 313-317

  Killenberg Jabs, Margrit ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Haploid yeast strain; Kinetics; Protein purification; Pyruvate decarboxylase; Thiamine diphosphate

Indexed keywords

ENZYME ANTIBODY; FUNGAL PROTEIN; POLYCLONAL ANTIBODY; PYRUVATE DECARBOXYLASE; PYRUVIC ACID; TETRAMER;

ARTICLE; CONTROLLED STUDY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; FUNGUS MUTANT; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

AMMONIUM SULFATE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE DELETION; GENES, FUNGAL; HAPLOIDY; ISOENZYMES; KINETICS; MOLECULAR WEIGHT; PYRUVATE DECARBOXYLASE; SACCHAROMYCES CEREVISIAE;

FUNGI; SACCHAROMYCES CEREVISIAE; SIGMOIDEA;

EID: 0030018357     PISSN: 01773593     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Boiteux, A., Hess. B. (1970). Allosteric properties of yeast pyruvate decarboxylase. FEBS Lett. 9,293 - 296.
2. Bradford, M. M. (1976). A rapid sensitive method for the quantification of microgram of protein utilising the principle of protein-dye binding. Anal. Biochemistry 72,248 - 254.
3. Dyda, F., Furey, W., Swaminathan, S., Sax, M., Farrenkopf, B., Jordan, F. (1993). Catalytic centers in the thiamin diphosphate dependent enzyme pyruvate decarboxylase at 2.4 Å resolution. Biochemistry 32,6165 - 6170.
4. Eppendorfer, S., König, S., Golbik, R., Neef, H., Lehle, K., Jaenicke, R., Schellenberger, A., Hübner, G. (1993). Effects of metal ions, thiamin diphosphate analogues subunit interactions on the reconstitution behaviour of pyruvate decarboxylase from brewer's yeast. Biol. Chem. Hoppe-Seyler 374,1129-1134.
5. Farrenkopf, B.C., Jordan, F. (1992). Resolution of brewer's yeast pyruvate decarboxylase into multiple isoforms with similar subunit structure activity using high-performance liquid chromatography. Protein Expression Purification 3,101 -107.
6. Gounaris, A.D., Turkenkopf, I., Civerchia, L.I., Greenlie, J. (1975). Pyruvate Decarboxylase III. Specifity restrictions for thiamine pyrophosphate in the protein association step, subunit structure. BBA 405,492 - 499.
7. Hohmann, S. (1991 a). Characterization of PDC6, a third structural gene for pyruvate decarboxylase in Saccharomyces cerevisiae. Journal of Bacteriology 173,7963 - 7969.
8. Hohmann, S. (1991 b). Structure expression of yeast pyruvate decarboxylase structural genes. In: Biochemistry Physiology of Thiamin Diphosphate Enzymes, H. Bisswanger J. Ullrich, eds. (Weinheim, Germany: Verlag Chemie), pp. 106-114.
9. Hohmann, S., Cederberg, H. (1990). Autoregulation may control the expression of yeast pyruvate decarboxylase structural genes PDC1 PDC5. Eur. J. Biochem. r88,615 - 621.
10. Holzer, H., Schultz, G., Villar-Palasi, C., Jüntgen-Sell, J. (1956). Isolierung der Hefecarboxylase und Untersuchung über die Aktivität des Enzyms in lebenden Zellen. Biochem. Z. 327,331-344.
11. Hübner, G., Schellenberger, A. (1970). Zur Theorie der Thiaminpyrophosphatwirkung. X. Untersuchungen zum Mechanismus der Produkthemmung bei der PDC-Reaktion. Hoppe-S. Z. Physiol. Chem. 351,1435 -1440.
12. Hübner, G., Schellenberger, A. (1986). Pyruvate decarboxylase - potentially inactive in the absence of the substrate. Biochem. Internat. 13,767 - 772.
13. Hübner, G., Weidhase, R., Schellenberger, A. (1978). The mechanism of substrate activation of pyruvate decarboxylase: A first approach. Eur. J. Biochem. 92,175 -181.
14. König, S., Svergun, D., Koch, M.H.J., Hübner, G., Schellenberger, A. (1992). Synchrotron radiation solution X-ray scattering study of the pH dependence of the quarternary structure of yeast pyruvate decarboxylase. Biochemistry 37, 8726-8731.
15. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 -685.
16. Lee, T.C., Langston-Unkefer, RJ. (1985). Pyruvate decarboxylasefrom Zea mays L. 1. Purification partial characterization from mature kernels anaerobically treated roots. Plant Physiol. 79,242-247.
17. Mücke, U., König, S., Hübner, G. (1995). Purification characterisation of PDG from pea seeds (Pisum sativum cv. Miko). Biol. Chem. Hoppe-Seyler 376,111-117.
18. Neale, A.D., Scopes, R.K., Wettenhall, R.E.H., Hoogenraad, N.J. (1987). Pyruvate decarboxylase of Zymomonas mobilis: Isolation, properties, genetic expression in £coll. J. of Bacteriol. 769,1024-1028.
19. Neuberg, C., Karczag, L (1911). Über zuckerfreie Hefegärung. IV. Carboxylase, ein neues Enzym der Hefe. Biochem.Z.36,68-75.
20. Schmitt, H.D., Zimmermann, F.K. (1982). Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis. J. of Bacteriol. 757,1146-1152.
21. Sieber, M., König, S., Hübner, G., Schellenberger, A. (1983). A rapid procedure for the preparation of highly purified pyruvate decarboxylase from brewer's yeast. Biomed. Biochim. Acta42, 343-349.
22. Ullrich, J., Donner, I. (1970). Kinetic evidence for two active sites in cytoplasmic yeast pyruvate decarboxylase. Hoppe-S. Z. Physiol. Chem. 357,1026 -1029.
23. Ullrich, J., Kellermann, J., Schmidt, W. (1991). Homotetrameric pyruvate decarboxylase (PDC; E.G. 4.1.1.1) from ABYS1 yeast: C- N-terminals some kinetic properties Biochem. Physiol. TOP Enzymes (ED. Bisswanger, Ullrich), 99-105.
24. Rivoal, J., Ricard, B., Pradet, A. (1990). Purification partial characterization of pyruvate decarboxylase from Oryza sativa L Eur. J. Biochem. 794,791 - 797.
25. Wright, A.P.H., Png, H.L., Hartley, B.S. (1989). Identification, cloning characterization of a new gene required for full pyruvate decarboxylase activity in S. cerevisiae. Curr. Genet. 75,171.
26. Zehender, H., Trescher, D., Ullrich, J. (1987). Improved purification of pyruvate decarboxylase from wheat germ. Its partial characterisation comparision with the yeast enzyme. Eur. J. Biochem. 767,149-154.
27. Zeng, X., Farrenkopf, B., Hohmann, S., Dyda, F., Furey, W., Jordan, F. (1993). Role of cysteines in the activation inactivation of brewers' yeast pyruvate decarboxylase investigated with aPDC1-PDC6 fusion protein. Biochemistry 32,2704 - 2709.