Carbon isotope effects on k(cat) for formate dehydrogenase determined using a continuous-flow stirred-tank reactor

Journal of the American Chemical Society

Volumn 118, Issue 24, 1996, Pages 5804-5805

  Xue, Hao ^a   Wu, Xiaowei ^a   Huskey, W Phillip ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FORMATE DEHYDROGENASE;

ARTICLE; ENZYME ACTIVITY; ISOTOPE LABELING; YEAST;

EID: 0030017879     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja960417y     Document Type: Article

References (11)

1. Parkin, D. W. In Isotope Effects from Enzyme Mechanism; Cook, P. F., Ed.; CRC Press: Boca Raton, FL, 1991; Chapter 11.
2. Nauman, E. B. Chemical Reactor Design; Wiley: New York, 1987.
3. Yeast formate dehydrogenase from Xylaria digitata (Candida boidinii) was used as obtained (lyophilized powder) from Boehringer Mannheim.
4. Blanchard, J. S.; Cleland, W. W. Biochemistry 1980, 19, 3543-3550.
5. Hermes, J. D.; Morrical, S. W.; O'Leary, M. H.; Cleland, W. W. Biochemistry 1984, 23, 5479-5488.
6. +, and 100 mL of formate dehydrogenase (0.25 mg/mL). These values are the concentrations and volumes of the feed solutions for the reactor.
7. 13C substrates.
8. In a few instances, we have noticed bubbles trapped in the reactor. If bubbles cannot escape, the volume of the reactor (and therefore the residence time) will change making it difficult to reach a good steady state.
9. LaReau, R. D.; Wan, W.; Anderson, V. E. Biochemistry 1989, 28, 3619-3624.
10. Gawlita, E.; Paneth, P.; Anderson, V. E. Biochemistry 1995, 34, 6050-6058.
11. Gawlita, E.; Caldwell, W. S.; O'Leary, M. H.; Paneth, P.; Anderson, V. E. Biochemistry 1995, 34, 2577-2583.