Fluorescence-optical measurements of chloride movements in cells using the membrane-permeable dye diH-MEQ

Pflugers Archiv European Journal of Physiology

Volumn 432, Issue 3, 1996, Pages 486-493

  Wöll, Ewald ^a   Gschwentner, Martin ^a   Fürst, Johannes ^a   Hofer, Sabine ^a   Buemberger, Gabriele ^a   Jungwirth, Andreas ^b   Frick, Julian ^b   Deetjen, Peter ^a   Paulmichl, Markus ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

^b SLKA   (Austria)

Author keywords

Chloride concentration; DIDS; Fluorescence; Fluorescence optical measurements; I(Cln); MEQ; MQAE; NPPB; SPQ

Indexed keywords

4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; 5 NITRO 2 (3 PHENYLPROPYLAMINO)BENZOIC ACID; ANTISENSE OLIGODEOXYNUCLEOTIDE; CHLORIDE CHANNEL; DYE;

ARTICLE; CELL MEMBRANE PERMEABILITY; CELL SWELLING; CHLORIDE CURRENT; CHLORIDE TRANSPORT; FIBROBLAST; FLUORESCENCE; HUMAN; HUMAN CELL; MEASUREMENT; PRIORITY JOURNAL;

3T3 CELLS; 4,4'-DIISOTHIOCYANOSTILBENE-2,2'-DISULFONIC ACID; ANIMALS; CELL SIZE; CHLORIDE CHANNELS; CHLORIDES; ELECTROPHYSIOLOGY; FLUORESCENT DYES; MICE; NITROBENZOATES; OLIGONUCLEOTIDES, ANTISENSE; PATCH-CLAMP TECHNIQUES; QUINOLINIUM COMPOUNDS;

EID: 0030017643     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050160     Document Type: Article

References (17)

1. Biwersi J, Verkman AS (1991) Cell-permeable fluorescent indicator for cytosolic chloride. Biochemistry 30 : 7879-7883
2. Bubien JK, Kirk KL, Rado A, Frizzell RA (1990) Cell cycle dependence of chloride permeability in normal and cystic fibrosis lymphocytes. Science 248 : 1416-1419
3. - channels in lymphocytes (abstract). Biophys J 66 : A3
4. Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M (1987) Lipofectin : a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA 84 : 7413-7417
5. Gschwentner M, Wöll E, Schmarda A, Kanin G, Ritter M, Paulmichl M (1993) Further characterization of a cloned epithelial chloride channel and its role in cell volume regulation (RVD) (abstract). Pflügers Arch 422 : R64
6. Gschwentner M, Susanna A, Wöll E, Ritter M, Nagl UO, Schmarda A, Laich A, Pinggera GM, Ellemunter H, Huemer H, Deetjen P, Paulmichl M (1995) Antiviral drugs from the nucleoside analog family block volume-activated chloride channels. Mol Med 4 : 1-11
7. Gschwentner M, Nagl UO, Wöll E, Schmarda A, Ritter M, Paulmichl M (1995) Antisense oligonucleotides suppress cell volume-induced activation of chloride channels. Pflügers 430 : 464-470
8. 29 cells: ion selectivity, additivity and stilbene sensitivity. Pflügers Arch 421 : 447-454
9. Kubo M, Okada Y (1992) Volume-regulatory C1 channel currents in cultured human epithelial cells. J Physiol (Lond) 456 : 351-371
10. Lang F, Völkl H, Häussinger D (1990) General principles in cell volume regulation. Comp Physiol 4 : 1-25
11. MacVinish LJ, Reancharoen T, Cuthbert AW (1993) Kinin-induced chloride permeability changes in colony 29 epithelia estimated from 125I-efflux and MEQ fluorescence. Br J Pharmacol 108 : 469-478
12. Marty A, Neher R (1983) Tight-seal whole-cell recording. In : Sakmann B, Neher E (eds) Single-channel recording. Plenum New York, pp 107-122
13. Paulmichl M, Lang F (1988) Enhancement of intracellular calcium concentration by extracellular ATP and UTP in Madin Darby canine kidney cells. Biochem Biophys Res Commun 156 : 1139-1143
14. Paulmichl M, Li Y, Wickman K, Ackerman M, Peralta E, Clapham D (1992) New mammalian chloride channel identified by expression cloning. Nature 356 : 238-241
15. Paulmichl M, Gschwentner M, Wöll E, Schmarda A, Ritter M, Kanin G, Ellemunter H, Waitz W, Deetjen P (1993) Insight into the structure-function relation of chloride channels. Cell Physiol Biochem 3 : 374-387
16. Verkman AS (1990) Development and biological applications of chloride-sensitive indicators. Am J Physiol 259 : C375-C388
17. Worrell RT, Butt GA, Cliff WH, Frizzell RA (1989) A volume-sensitive chloride conductance in human colonic cell line T84. Am J Physiol 256 : C1111-C1119