Punctuated evolution caused by selection of rare beneficial mutations

Science

Volumn 272, Issue 5269, 1996, Pages 1802-1804

  Elena, Santiago F ^a   Cooper, Vaughn S ^a   Lenski, Richard E ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL SIZE; ESCHERICHIA COLI; EVOLUTION; GENE MUTATION; NATURAL SELECTION; NONHUMAN; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0030017289     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5269.1802     Document Type: Article

References (32)

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29. We measured average cell size using a Coulter particle counter (model ZM and channelyzer model 256), which measures the volume displaced by each of several thousand cells as they pass through a small aperture. Cells were grown to stationary-phase density in the same medium used in the evolution experiment (6, 8, 14). Replicate assays were run in complete blocks to avoid possible confounding effects of temporal variation in experimental conditions.
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31. The Levenberg-Marquard numerical method used to fit the models to the data requires an initial value for each parameter, several sets of initial values were tested for convergence
32. We thank J. A. Mongold for help with the experiments; A. F. Bennett, J. E. Cohen, D. J. Hall, J. A. Mongold, and P. D. Sniegowski for discussion and comments; and A. G. Clark for suggesting that we obtain these data. This work was supported by a fellowship from the Spanish Ministerio de Educaciòn y Ciencia to S.F E., a fellowship from Michigan State University to V S.C , and a grant from NSF (DEB-9421237) to R.E.L