Substitution of L-fucose by L-galactose in cell walls of Arabidopsis mur1

Science

Volumn 272, Issue 5269, 1996, Pages 1808-1810

  Zablackis, Earl ^a   York, William S ^a   Pauly, Markus ^a   Hantus, Stephen ^a   Reiter, Wolf Dieter ^b   Chapple, Clint C S ^c   Albersheim, Peter ^a   Darvill, Alan ^a  

^a University of Georgia ^*  (United States)

^b UNIVERSITY OF CONNECTICUT   (United States)

^c PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUCOSE; GALACTOSE;

ARABIDOPSIS; ARTICLE; CELL WALL; CONFORMATIONAL TRANSITION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

ARABIDOPSIS; ARABIDOPSIS MURI; ARABIDOPSIS THALIANA;

EID: 0030017113     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5269.1808     Document Type: Article

References (34)

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8. Cell walls of mur1 plants were prepared and XG was isolated by the procedures used for wild-type XG (9). XG subunit oligosaccharides from mur1 were generated (9) and then separated on an analytical CarboPac-l PA1 anion-exchange column installed in a Dionex BioLC system. The column was eluted at a rate of 1 ml/min with 50 mM NaOAc in 100 mM NaOH (0 to 1 1 min) followed by a linear gradient of 50 to 70 mM NaOAc in 100 mM NaOH (1 1 to 40 mm). The oligosaccharides in the eluant were monitored by pulsed amperometric detection Masses of the subunit oligosaccharides were determined by matrix-assisted laser desorption (MALDI)-time of flight (TOF) MS, performed on a Hewlett Packard LDI 1700XP spectrometer (19). 2,5-Dihydroxybenzoic acid was used as the matrix All determined masses were within 0 1% of the calculated chemical mass. Glycosyl-residue and glycosyl-linkage compositions of the oligosacchandes were determined as described (20) Partially methylated alditol acetate derivatives of t-Xylp, 2-Xylp, t-Galp, 2-Galp, 6-Glcp, 4,6-Glcp, and 4-glucitot (4-Glcol) were recovered from XLJGol (12) in a molar ratio of 1:2:2:0.8:1.3:2:0.7. Relative amounts of XG subunit oligosaccharides were determined by reversed-phase high-performance liquid chromatography (HPLC) of their N-(p-nitrobenzyloxy)-arninoalditol derivatives (21), the structures of which were confirmed by MALDI-TOF MS.
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13. 4 and acetylated (22). The FAB mass spectrum was recorded with a JEOL JMS-SX/SX102A tandem mass spectrometer operating at low resolution (1:1000) with an accelerating voltage of 10 KV
14. 1H-NMR spectrum recorded at 300 K as described (22)
15. The absolute configurations (D and L) of the galactosyl residues in the mur1 XG oligosaccharides were determined by capillary GC analysis, on a fused silica DB-1 column (20), of their trimethylsilylated (+ or -) -2-butyl α- and β-glycoside derivatives (23). D- and L-Gal were present in a 2 · 1 ratio. A mixture of authentic D-Xyl, D-glucose (D-Glc), D-Gal, and L-Gal in a molar ratio of 3:4-2:1 produced a GC profile identical to that obtained from authentic XLJG.
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31. XG oligosaccharide subunits were obtained from wild-type or mur1 plants of A. thaliana, or both (8). The letter codes identifying the subunits follow the accepted XG nomenclature (24) The letter J is used to represent the β-D-Glcp- residue bearing an α-L-Galp-β-D-Galp-α-D-Xylp side chain, first detected in jojoba seed XG (25).
32. a is the glycosyl residue in XLJGol bearing the α-L-Gal residue at O-2. The α-L-Galp resonances not listed in Table 2 were assigned as follows: H-3 at δ 3.890, H 4 at δ 3.998, H-5 at δ 4.409, and H-6/H-6′ at δ 3.767 ppm.
33. 2O by centrifugation XG was extracted from the washed powder with 4 M KOH, and oligosaccharide subunits were generated and purified from the neutral fraction (9). The pea stem elongation assay was performed essentially as described (18), except stems were taken from seedlings after onset of development of the 3rd node (in preparation)
34. We thank C R. Somerville for the mur1 mutant and J. Huang, C. Chambers, and C McWhorter for help in purifying the XG oligosaccharides. This research was supported by the U.S. Department of Energy (DOE)-funded (DE-FG05-93ER20097) Center for Plant and Microbial Complex Carbohydrates, DOE grants DE-FG05-93ER20115 (to A.D ) and DE-FG05-93ER20114 (to P A.), USDA grant 93-37304-9510 (to (W -D R), DOE grant DE-FG02-95ER20203 (to W.-D R), and NSF grant MCB-9405739 (to W.-D.R.).