Functional characterization of a sialyltransferase-deficient mutant of Neisseria gonorrhoeae

Infection and Immunity

Volumn 64, Issue 8, 1996, Pages 3374-3378

  Gill, Martin J ^a   Mcquillen, Daniel P ^b   Van Putten, Jos P M ^c   Wetzler, Lee M ^b   Bramley, Jennifer ^a   Crooke, Helen ^a   Parsons, Nicholas J ^a   Cole, Jeff A ^a   Smith, Harry ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b BOSTON MEDICAL CENTER   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENT COMPONENT C3; SIALYLTRANSFERASE;

ARTICLE; BACTERIAL VIRULENCE; BACTERICIDAL ACTIVITY; COMPLEMENT ACTIVATION; COMPLEMENT FIXATION; ENZYME ANALYSIS; ENZYME DEFICIENCY; NEISSERIA GONORRHOEAE; NONHUMAN; OPSONIZATION; PHAGOCYTOSIS; PRIORITY JOURNAL; SIALYLATION;

EID: 0030017019     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.8.3374-3378.1996     Document Type: Article

References (23)

1. Bramley, J., R. Demarco de Hormaeche, C. Constantinidou, X. Nassif, N.J. Parsons, P. Jones, J. A. Cole, and H. Smith. 1995. A serum-sensitive, sialyl-transterase-deficient mutant of Neisseria gonorrhoeae defective in conversion to serum resistance by CMP-NANA or Wood cell extracts. Microb. Pathog. 18:187-195.
2. Elkins, C., N. H. Carbonetti, V. A. Varela, D. Stirewalt, D. G. Klapper, and P. F. Sparling. 1992. Antibodies to N-terminal peptides of gonococcal porin are bactericidal when gonococcal lipopolysaccharide is not sialylated. Mol. Microbiol. 6:2617-2628.3.
3. Hammer, C. H., G. H. Wirtz, L. Renfer, H. D. Gresham, and B. F. Tack. 1981. Large scale isolation of functionally active components of the human complement system. J. Biol. Chem. 256:3995-4006.
4. Kim, J. J., D. Zhou, R. E. Mandrell, and J. M. Griffiss. 1992. Effect of exogenous sialylation of lipooligosaccharide of Neisseria gonorrhoeae on opsonophagocytosis. Infect. Immun. 60:4439-4442.
5. Mandrell, R. E., J. M. Griffiss, H. Smith, and J. A. Cole. 1993. Distribution of a lipooligosaccharide-specific sialyltransferase in pathogenic and nonpathogenic Neisseria. Microb. Pathog. 14:315-327.
6. Mandrell, R. E., A. J. Lesse, J. V. Sugai, J. M. Shero, J. M. Griffiss, J. A. Cole, N. J. Parsons, H. Smith, S. A. Morse, and M. A. Apicella. 1990. In vitro and in vivo modifications of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation. J. Exp. Med. 171:1649-1664.
7. Mandrell, R. E., H. Smith, G. A. Jarvis, J. M. Griffiss, and J. A. Cole. 1993. Detection and some properties of the sialyltransferase implicated in the sialylation of the lipopolysaccharide of Neisseria gonorrhoeae. Microb. Pathog. 14:307-313.
8. Morse, S. A., S. Stein, and J. Hines 1974. Glucose metabolism in Neisseria gonorrhoeae. J. Bacteriol. 129:702-714.
9. r blood cell fractions that confer serum resistance on gonococci. Microb. Pathog. 14:329-336.
10. Parsons, N. J., G. Boons, P. R. Ashton, P. D. Redfern, P. Quirk, V. Gao, C. Contstantinidou, J. Patel, J. Bramley, J. A. Cole, and H. Smith. 1996. Lactic acid is the factor in blood cell extracts which enhances the ability of CMPNANA to sialylatc gonococcal lipopolysaccharide and induce serum resistance. Microb. Pathog. 20:87-100.
11. Patel, P. V., N. J. Parsons, J. R. C. Andrade, C. A. Nairn, E. L. Tan, M. Goldner, J. A. Cole, and H. Smith. 1988. White blood cells including polymorphonuclcar phagocytes contain a factor which induces gonococcal resistance to complement mediated killing. FEMS Microbiol. Lett. 50:173-176.
12. Rest, R. F., and J. V. Frangipane. 1992. Growth of Neisseria gonorrhoeae in CMP-N-acetylneuraminic acid inhibits nonopsonic (opacity-associated outer membrane protein-mediated) interactions with human neutrophils. Infect. Immun. 60:989-997.
13. Ross, S. C., and P. Densen. 1995. Opsonophagocytosis of Neisseria gonor rhoeae: interaction of local and disseminated isolates with complement and neutrophils. J. Infect. Dis. 151:33-41.
14. Schneider, H., A. S. Cross, R. A. Kuschner, D. N. Taylor, J. G. Sadoff, J. W. Boslego, and C. D. Deal. 1995. Experimental human gonococcal urethritis: 250 Neisseria gonorrhoeae MS11mkC are infective. J. Infect. Dis. 122:180-185.
15. Smith, H., N. J. Parsons, and J. A. Cole. 1995. Sialylation of neisserial lipopolysaccharide: a major influence on pathogenicity. Microb. Pathog. 19:365-377.
16. Van Putten, J. P. M. 1993. Phase variation of lipopolysaccharide directs interconversion of invasive and immunoresistant phenotypes of Neisseria gonorrhoeae. EMBO J. 12:4043-4051.
17. Van Putten, J. P. M., and S. M. Paul. 1995. Binding of syndecan-like cell surface proteoglyean receptors is required for Neisseria gonorrhoeae entry into human mucosal cells. EMBO J. 14:2144-2154.
18. Van Pulten, J. P. M., J. F. L. Weel, and H. U. C. Grassme. 1994. Measurements of invasion by antibody labelling and electron microscopy. Methods Enzymol. 236:420-437.
19. Veale, D. R., C. W. Penn, and H. Smith. 1981. Factors affecting the induction of phenotypically determined serum resistance of Neisseria gonorrhoeae grown in media containing serum and its diffusible components. J. Gen. Microbiol. 122:235-245.
20. Wetzler, L. M., K. Barry, M. S. Blake, and E. C. Gotschlich. 1992. Gonococcal lipooligosaccharide sialylation prevents complement-dependent killing by immune sera. Infect. Immun. 60:39-43.
21. Wetzler, L. M., M. S. Blake, and E. C. Gotschlich. 1988. Characterization and specificity of antibodies to protein 1 of Neisseria gonorrhoeae produced by injection with various protein 1 adjuvant preparations. J. Exp. Med. 168:1883-1897.
22. Wetzler, L M., E. C. Gotschlich, M. S. Blake, and J. M. Koomey. 1989. The construction and characterization of Neisseria gonorrhoeae lacking PIII in its outer membrane. J. Exp. Med. 169:2199-2209.
23. Zaleski, T., and P. Densen. 1994. Effect of Gc sialylation on antibody deposition and complement activation, p. 241-242. In J. S. Evans, S. E. Yost, M. C. J. Maiden, and M. I. Feavers (ed.). Neisseria 94. National Institute for Biological Standards and Control, Potters Bar, United Kingdom.