Adenosine diphosphate as an intracellular regulator of insulin secretion

Science

Volumn 272, Issue 5269, 1996, Pages 1785-1787

  Nichols, C G ^a   Shyng, S L ^a   Nestorowicz, A ^a   Glaser, B ^b   Clement IV, J P ^c   Gonzalez, G ^c   Aguilar Bryan, L ^c   Permutt, M A ^a   Bryan, J ^c  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b HEBREW UNIVERSITY   (Israel)

^c BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; POTASSIUM CHANNEL;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CHANNEL GATING; GLUCOSE BLOOD LEVEL; INSULIN RELEASE; NONHUMAN; POTASSIUM CELL LEVEL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ANIMALIA; CRICETINAE;

EID: 0030016913     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5269.1785     Document Type: Article

References (21)

1. F M Ashcroft, Annu. Rev. Neurosci. 11, 97 (1988).
2. M. J. Dunne and O. Petersen, FEBS Lett 208, 59 (1986).
3. S. Misler, L C. Falke, K. Gillis, M. L McDaniel, Proc Natl Acad Sci. U.S.A. 83, 7119 (1986)
4. L Aguilar-Bryan et al. Science 268, 423 (1995)
5. N. Inagaki et al., ibid 270, 1166 (1995).
6. P. M. Thomas et al , ibid. 268, 426 (1995).
7. K. Zuppinger, Prog. Pediatr. Surg. 16. 51 (1983).
8. M P. Anderson and M. J. Welsh, Science 257, 1701 (1992).
9. L S. Smit, D. J. Wilkinson, M. K Mansoura, F S. Collins, D. C. Dawson, Proc. Natl. Acad Sci. U.S.A. 90, 9963 (1993).
10. Mutant constructs were prepared by overlap extension at the junctions of the relevant domains by sequential polymerase chain reaction (PCR). The resulting PCR products were subcloned into pECE vector [L Ellis et al., Cell 45, 721 (1986)] and sequenced to verify the correct mutant construct before transfection
11. 5 cells per well (30-mm, six-well dishes) and cultured in Dulbecco's modified Eagle's medium plus 10 mM glucose (DMEM-HG) supplemented with fetal calf serum (FCS, 10%) The following day Kir6.2 (5 μ.g) and pECE-haSUR complementary DNA (cDNA) (5 μg) were cotransfected into the COSm6 cells with diethylaminoethyl dextran (5 mg/ml). Cells were incubated for 2 min in Hepes-buffered salt solution containing dimethyl sulfoxide (10%), then for 4 hours in DMEM-HG plus 2% FCS and chloroquine (100 μM), and then returned to DMEM-HG plus 10% FCS
12. + released.
13. 2+] were made with a program written by M. Kurzmak (Department of Biological Chemistry, University of Maryland), which was based on the formulations of A. Fabiato and F. Fabiato [J. Physiol. (Paris) 75, 463 (1979)].
14. W J. Lederer and C. G Nichols J Physiol. 419, 193 (1989).
15. C G. Nichols et al., data not shown.
16. W. F. Hopkins, S Fatherazi, B. Peter-Riesch, B. E. Corkey, D. L. Cook, J Membr. Biol 129, 287 (1992).
17. C. G. Nichols, W J. Lederer, M. B. Cannell, Biophys. J. 60, 1164 (1991).
18. J. E Walker, M Saraste, M. J. Runswick, N. J. Gay. EMBO J 1, 945 (1982)
19. H Baubichon-Cortay, L G Baggetto, G. Dayan, A. DiPietro, J. Biol. Chem. 269, 22983 (1994).
20. We made the sequence alignments with Lasergene Megalign software (DNAStar, Madison, WI) using the Clustal method with standard weighting parameters.
21. We are grateful to A. N Lopatin, E N Makhina, E. A. Wilson, and K. P. Schoor for help with experiments. Supported by grants from NIH (to C G.N., M.A.P., and J.B), from the Juvenile Diabetes Fund (to C.G.N. and JB), and from the American Diabetes Association Houston Endowment (to L A.B.).