Synthesis of Semliki Forest virus RNA polymerase components nsP1 through nsP4 in Saccharomyces cerevisiae by expression of cDNA encoding the nonstructural polyprotein

Journal of Virology

Volumn 70, Issue 6, 1996, Pages 4086-4089

  Russo, Patrick ^a   Laakkonen, Pirjo ^a   Ahola, Tero ^a   Kääriäinen, Leevi ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; ENZYME SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA REPLICATION; SACCHAROMYCES CEREVISIAE; SEMLIKI FOREST ALPHAVIRUS;

EID: 0030014175     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.6.4086-4089.1996     Document Type: Article

References (24)

1. Ahola, T., and L. Kääriäinen. 1995. Reaction in alphavirus mRNA capping: formation of a covalent complex of nonstructural protein nsP1 with 7-methyl-GMP. Proc. Natl. Acad. Sci. USA 92:507-511.
2. Barton, D. J., S. G. Sawicki, and D. L. Sawicki. 1988. Demonstration in vitro of temperature-sensitive elongation of RNA in Sindhis virus mutant ts6. J. Virol. 62:3597-3502.
3. Barton, D. J., S. G. Sawicki, and D. L. Sawicki. 1991. Solubilization and immunoprecipitation of alphavirus replication complexes. J. Virol. 65:1496-1506.
4. Brunelli, J. P., and M. L. Pall. 1993. A series of yeast shuttle vectors for expression of cDNA and other DNA sequences. Yeast 9:1299-1308.
5. Froshauer, S., J. Kartenbeck, and A. Helenius. 1988. Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J. Cell Biol. 107:2075-2086.
6. Gomatos, P., L. Kääriäinen, S. Keränen, M. Ranki, and D. L. Sawicki. 1980. Semliki Forest virus replication complex capable of synthesizing 42S and 26S RNA chains. J. Gen. Virol. 49:61-69.
7. Ito, H., Y. Fukuda, K. Murata, and A. Kumura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
8. Jämsä, E., M. Simonen, and M. Makarow. 1994. Selective retention of secretory proteins in the yeast endoplasmic reticulum by treatment of cells with a reducing agent. Yeast 10:355-370.
9. Janda, M., and P. Ahlquist. 1993. RNA-dependent replication, transcription, and persistence of brome mosaic virus RNA replacons in S. cerevisiae. Cell 72:961-970.
10. Kääriäinen, L., K. Takkinen, S. Keränen, and H. Söderlund. 1987. Replication of the genome of alphaviruses. J. Cell Sci. Suppl. 7:231-250.
11. Laakkonen, P., M. Hyvönen, J. Peränen, and L. Kääriäinen. 1994 Expression of Semliki Forest virus nsP1-specific methyltransferase in insect cells and in Escherichia coli. J. Virol. 68:7418-7425.
12. Liljeström, P., and H. Garoff. 1991. A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Bio/Technology 9: 1356-1361.
13. Peränen, J., and L. Kääriäinen. 1991. Biogenesis of type 1 cytopathic vacuoles in Semliki Forest virus-infected BHK cells. J. Virol. 65:1623-1627.
14. Peränen, J., P. Laakkonen, M. Hyvönen, and L. Kääriäinen. 1995. The alphavirus replicase protein nsP1 is membrane-associated and has affinity to endocytic organelles. Virology 208:610-620.
15. Peränen, J., M. Rikkonen, P. Liljeström, and L. Kääriäinen. 1990. Nuclear localization of Semliki Forest virus-specific nonstructural protein nsP2. J. Virol. 64:1888-1896.
16. Peränen, J., K. Takkinen, N. Kalkkinen, and L. Kääriäinen. 1988. Semliki Forest virus-specific nonstructural protein, nsP3, is a phosphoprotein. J. Gen. Virol. 69:2165-2178.
17. Pringle, J. R., A. E. M. Adams, D. G. Drubin, and B. K. Haarer. 1991. Immunofluorescence methods in yeast. Methods Enzymol. 194:565-602.
18. Rikkonen, M., J. Peränen, and L. Kääriäinen. 1992. Nuclear and nucleolar targeting signals of Semliki Forest virus nonstructural protein nsP2. Virology 189:462-473.
19. Rikkonen, M., J. Peränen, and L. Kääriäinen. 1994. ATPase and GTPase activities associated with Semliki Forest virus nonstructural protein nsP2. J. Virol. 68:5804-5810.
20. Russo, P., and F. Sherman. 1989. Transcription terminates near the poly(A) site in the CYC1 gene of the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 86:8348-8352.
21. Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 194:3-21.
22. Strauss, J. H., and E. G. Strauss. 1994. The alphaviruses: gene expression, replication, and evolution. Microbiol. Rev. 58:491-562.
23. Takkinen, K., J. Peränen, and L. Kääriäinen. 1991. Proteolytic processing of Semliki Forest virus-specific non-structural polyprotein. J. Gen. Virol. 72: 1627-1633.
24. Zaret, K. S., and F. Sherman. 1982. DNA sequence required for efficient transcription termination in yeast. Cell 28:563-573.