Single-tube protocol for the extraction of DNA or RNA from paraffin-embedded tissues using a starch-based adhesive

BioTechniques

Volumn 20, Issue 5, 1996, Pages 846-853

  Turbett, Gavin R ^a   Barnett, Timothy C ^a   Dillon, Elizabeth K ^a,b   Sellner, Loryn N ^a  

^a ROYAL PERTH HOSPITAL   (Australia)

^b UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RNA; STARCH;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA PURIFICATION; EXTRACTION; FEMALE; HUMAN; HUMAN TISSUE; LABORATORY TEST; RNA ANALYSIS; RNA PURIFICATION;

EID: 0030013552     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205st04     Document Type: Article

References (6)

1. Bianchi, A.B., N.M. Navone and C.J. Conti. 1991. Detection of loss of heterozygosity in formalin-fixed paraffin-embedded tumor specimens by the polymerase chain reaction. Am. J. Pathol. 138:279-284.
2. Greer, C.E., S.L. Peterson, N.B. Kiviat and M.M. Manos. 1991. PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time. Am. J. Clin. Pathol. 95:117-124.
3. Gyapay, G., J. Morissette, A. Vignal, C. Dib, C. Fizams, P. Millasseau, S. Marc, G. Bernardi, M. Lathrop and J. Weissenbach. 1994. The 1993-94 Généthon human genetic linkage map. Nat. Genet. 7:246-339.
4. Shibata, D. 1993. Selective ultraviolet radiation fractionation and polymerase chain reaction analysis of genetic alterations. Am. J. Pathol. 143:1523-1526.
5. Shibata, D., D. Hawes, Z-H. Li, A.M. Hernandez, C.H. Spruck and P.W. Nichols. 1992. Specific genetic analysis of microscopic tissue after selective ultraviolet radiation fractionation and the polymerase chain reaction. Am. J. Pathol. 141:539-543.
6. Sundaresan, V., P. Ganly, P. Hasleton, N.M. Blechen and P. Rabbitts. 1993. Paraffin waxembedded material as a source of DNA for the detection of somatic genetic changes. J. Pathol. 169:43-52.