Uso1 protein is a dimer with two globular heads and a long coiled-coil tail

Journal of Structural Biology

Volumn 116, Issue 3, 1996, Pages 356-365

  Yamakawa, Hisashi ^a,b   Seog, Dae Hyun ^a   Yoda, Koji ^a   Yamasaki, Makari ^a   Wakabayashi, Takeyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KAZUSA DNA RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT;

ARTICLE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0030010763     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1996.0053     Document Type: Article

References (37)

1. Atkinson, S. J., and Stewart, M. (1991a) Molecular basis of myosin assembly: coiled-coil interactions and the role of charge periodicities, J. Cell Sci. Suppl. 14, 7-10.
2. Atkinson, S. J., and Stewart, M. (1991b) Expression in Escherichia coli of fragments of the coiled-coil rod domain of rabbit myosin: influence of different regions of the molecule on aggregation and paracrystal formation, J. Cell Sci. 99, 823-836.
3. Atkinson, S. J., and Stewart, M. (1992) Molecular interactions in myosin assembly: Role of the 28-residue charge repeat in the rod, J. Mol. Biol. 226, 7-13.
4. Barroso, M., Nelson, D. S., and Sztul, E. (1995) Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes, Proc. Natl. Acad. Sci. USA 92, 527-531.
5. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-250.
6. Côté, G. P., and McCrea, S. M. (1987) Selective removal of the carboxyl-terminal tail end of the Dictyostelium myosin II heavy chain by chymotrypsin, J. Biol. Chem. 262, 13033-13038.
7. Dascher, C., Ossig, R., Gallwitz, D., and Schmitt, H. D. (1991) Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily, Mol. Cell. Biol. 11, 872-885.
8. Elliott, A., and Offer, G. (1978) Shape and flexibility of the myosin molecule, J. Mol. Biol. 123, 505-519.
9. Kuczmarski, E. R., and Spudich, J. A. (1980) Regulation of myosin self-assembly: Phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments, Proc. Natl. Acad. Sci. USA 77, 7292-7296.
10. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
11. Lupas, A., Van Dyke, M., and Stock, J. (1991) Predicting coiled coils from protein sequences, Science 252, 1162-1164.
12. Maeda, K., Roesch, A., Maeda, Y., Kalbitzer, H. R., and Wittinghofer, A. (1991) Rabbit skeletal muscle myosin: Unfolded carboxyl-terminus and its role in molecular assembly, FEBS Lett. 281, 23-26.
13. McLachlan, A. D., and Karn, J. (1982) Periodic charge distributions in the myosin rod amino acid sequence match cross-bridge spacings in muscle, Nature 299, 226-231.
14. Nair, J., Müller, H., Peterson, M., and Novick, P. (1990) Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain, J. Cell Biol. 110, 1897-1909.
15. Nakajima, H., Hirata, A., Ogawa, Y., Yonehara, T., Yoda, K., and Yamasaki, M. (1991) A cytoskeleton-related gene, USO1, is required for intracellular protein transport in Saccharomyces cerevisiae, J. Cell Biol. 113, 245-260.
16. Nakano, A., and Muramatsu, M. (1989) A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus, J. Cell Biol. 109, 2677-2691.
17. Newman, A. P., and Ferro-Novick, S. (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection, J. Cell Biol. 105, 1587-1594.
18. Newman, A. P., Shim, J., and Ferro-Novick, S. (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex, Mol. Cell Biol. 10, 3405-3414.
19. Novick, P., Ferro, S., and Schekman, R. (1981) Order of events in the yeast secretory pathway, Cell 25, 461-469.
20. Novick, P., Field, C., and Schekman, R. (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway, Cell 21, 205-215.
21. Nyitray, L., Mocz, G., Szilagyi, L., Balint, M., Lu, R. C., Wong, A., and Gergely, J. (1983) The proteolytic substructure of light meromyosin: Localization of a region responsible for the low ionic strength insolubility of myosin, J. Biol. Chem. 258, 13213-13220.
22. O'Halloran, T. J., Ravid, S., and Spudich, J. A. (1990) Expression of Dictyostelium myosin tail segments in Escherichia coli. Domains required for assembly and phosphorylation, J. Cell Biol. 110, 63-70.
23. Onishi, H., and Wakabayashi, T. (1982) Electron microscopic studies of myosin molecules from chicken gizzard muscle I: The formation of the intramolecular loop in the myosin tail, J. Biochem. 92, 871-879.
24. Palade, G. (1975) Intracellular aspects of the process of protein synthesis, Science 189, 347-358.
25. Parry, D. A. D. (1981) Structure of rabbit skeletal myosin: Analysis of the amino acid sequences of two fragments from the rod region, J. Mol. Biol. 153, 459-464.
26. Pryer, N. K., Wuestehube, L. J., and Schekman, R. (1992) Vesicle-mediated protein sorting, Annu. Rev. Biochem. 61, 471-516.
27. Ravid, S., and Spudich, J. A. (1989) Myosin heavy chain kinase from developed Dictyostelium cells, J. Biol. Chem. 264, 15144-15150.
28. Rothman, J. E., and Orci, L. (1992) Molecular dissection of the secretory pathway, Nature 355, 409-415.
29. Sapperstein, S. K., Walter, D. M., Grosvenor, A. R., Heuser, J. E., and Waters, M. G. (1995) p115 is a general vesicular transport factor related to the yeast ER-Golgi transport factor Uso1p, Proc. Natl. Acad. Sci. USA 92, 522-526.
30. Segev, N., Mulholland, J., and Botstein, D. (1988) The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery, Cell 52, 915-924.
31. Seog, D.-H., Kito, M., Igarashi, K., Yoda, K., and Yamasaki, M. (1994a) Molecular characterization of the USO1 gene product which is essential for vesicular transport in Saccharomyces cerevisiae, Biochem. Biophys. Res. Commun. 200, 647-653.
32. Seog, D.-H., Kito, M., Yoda, K., and Yamasaki, M. (1994b) Uso1 protein contains a coiled-coil rod region essential for protein transport from the ER to the Golgi apparatus in Saccharomyces cerevisiae, J. Biochem. 116, 1341-1345.
33. 2-and COOH-terminal deletions, J. Cell Biol. 111, 2417-2426.
34. Stearns, T., Willingham, M. C., Botstein, D., and Kahn, R. A. (1990) ADP-ribosylation factor is functionally and physically associated with the Golgi complex, Proc. Natl. Acad. Sci. USA 87, 1238-1242.
35. Tyler, J. M., and Branton, D. (1980) Rotary shadowing of extended molecules dried from glycerol, J. Ultrastruct. Res. 71, 95-102.
36. Waters, M. G., Clary, D. O., and Rothman, J. E. (1992) A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack, J. Cell Biol. 118, 1015-1026.
37. Wrigley, N. G. (1968) The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy, J. Ultrastruct. Res. 24, 454-464.