Dual color microscopic imagery of cells expressing the green fluorescent protein and a red-shifted variant

Gene

Volumn 173, Issue 1, 1996, Pages 19-23

  Yang, Te Tuan ^a   Kain, Steven R ^a   Kitts, Paul ^a   Kondepudi, Anu ^a   Yang, Mary M ^b   Youvan, Douglas C ^b  

^a CLONTECH Laboratories Inc   (United States)

^b KAIROS Scientific Inc   (United States)

Author keywords

Aequorea victoria; Bioluminescence; Combinatorial mutagenesis; Digital imaging spectroscopy; Fluorescent protein; Gene expression; Protein localization; Signal transduction

Indexed keywords

FLUORESCENT DYE; MUTANT PROTEIN;

ANIMAL CELL; CONFERENCE PAPER; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; JELLYFISH; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN VARIANT;

AEQUOREA VICTORIA; ANIMALIA; MAMMALIA; RENILLA MULLERI; RENILLA RENIFORMIS; VICTORIA; VIOLA (ANGIOSPERM);

EID: 0030010676     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(95)00781-4     Document Type: Conference Paper

References (14)

1. Adams, S.R., Harootunian, A.T., Buechler, Y.J., Taylor, S.S. and Tsien, R.Y.: Fluorescence ratio imaging of cyclic AMP in single cells. Nature 349 (1991) 694-697.
2. Chalfie, M., Tu, Y.T., Euskirchen, G., Ward, W.W. and Prasher, D.C.: Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-805.
3. Cody, C.W., Prasher, D.C., Westler, W.M., Prendergast, F.G. and Ward, W.W.: Chemical structure of the hexapeptide chromophore of the Aequorea green fluorescent protein. Biochemistry 32 (1993) 1212-1218.
4. Delagrave, S., Hawtin, R.E., Silva, C.M., Yang, M.M. and Youvan, D.C.: Red-shifted excitation mutants of the green fluorescent protein. Bio/ Technology 13 (1995) 151-154.
5. Deng, W.P. and Nickoloff, J.A.: Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200 (1992) 81-84.
6. Flach, J., Bossie, M, Vogel, J., Corbett, A., Jinks, T., Willins, D.A. and Silver, P.A.: A yeast RNA-binding protein shuttles between the nucleus and cytoplasm. Mol. Cell. Biol. 14 (1995) 8399-8407.
7. Heim, R., Prasher, D.C. and Tsien. R.Y.: Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91 (1994) 12501-12504.
8. Inouye, S. and Tsuji, F.I.: Aequorea green fluorescent protein: expresion of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341 (1994) 277-280.
9. Kozak, M.: An analysis of 5′-coding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15 (1987) 8125-8148.
10. Mitra, R.D., Silva, C.M. and Youvan, D.C.: Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 173 (1996) 13-17.
11. Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G. and Cormier, M.J.: Primary structure of the Aequorea victoria green fluorescent protein. Gene 111 (1992) 229-233.
12. Stearns, T.: The green revolution. Curr. Biol. 5 (1995) 262-264.
13. Wang, S. and Hazelrigg, T.: Implications for bed mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature 369 (1994) 400-403.
14. Ward, W.W. and Cormier, M.J. : Energy transfer via protein-protein interaction in Renilla bioluminescence. Photobiochem. Photobiol. 27 (1978) 389-396.