Solid-phase method for differential display of genes expressed in hematopoietic stem cells

BioTechniques

Volumn 21, Issue 1, 1996, Pages 114-121

  Rosok O ^a   Odeberg, J ^b   Rode, M ^a   Stokke, T ^a   Funderud, S ^a   Smeland, E ^a   Lundeberg, J ^a,b  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL DIFFERENTIATION; CELL ISOLATION; GENE EXPRESSION; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; SOLID PHASE EXTRACTION;

EID: 0030010546     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96211rr02     Document Type: Article

References (18)

1. Åsheim, H.-C., A. Deggerdal, E.B. Smeland and E. Hornes. 1994. A simple subtraction method for the isolation of cell-specific genes using magnetic monodisperse polymer particle. BioTechniques 16:716-721.
2. Bauer, D., H. Müller, J. Reich, H. Riedel, V. Ahrenkiel, P. Warthoe and M. Strauss. 1993. Identification of differentially expressed mRNA species by improved display technique (DDRT-PCR). Nucleic Acids Res. 21:4272-4280.
3. Callard, D., B. Lescure and L. Mazzolini. 1994. A method for the elimination of false positives generated by the mRNA differential display technique. BioTechniques 16:1096-1103.
4. Chen, Z., K. Swissheim and R. Sager. 1994. A cautionary note on reaction tubes for differential display and cDNA amplification in thermal cycling. BioTechniques. 16:1003-1006.
5. Hedrum, A., F. Pontén, Z. Ren, J. Lundeberg, J. Pontén and M. Uhlén. 1994. Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples. BioTechniques 17:118-129.
6. Hultman, T., S. Bergh, T. Moks and M. Uhlén. 1991. Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. BioTechniques 10:84-93.
7. Hultman, T., S. Ståhl, E. Hornes and M. Uhlén. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 17:4937-4946.
8. Lambert, K.N. and V.M. Williamson. 1993. cDNA library construction from small amounts of RNA using paramagnetic beads and PCR. Nucleic Acids Res. 21:775-776.
9. Lee, Y-H. and V.D. Vacquier. 1992. Reusable cDNA libraries coupled to magnetic beads. Anal. Biochem. 206:206-207.
10. Li, F., E.S. Barnathan and K. Kariko. 1994. Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of Northern blot for affinity capturing of cDNAs. Nucleic Acids Res. 22:1764-1765.
11. Liang, P., L. Averboukh, K. Keyomarsi, R. Sager and A.B. Pardee. 1992. Differential display and cloning of mRNAs from human breast cancer versus mammary epithelial cells. Cancer Res. 52:6966-6968.
12. Liang, P., L. Averboukh and A.B. Pardee. 1993. Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimisations. Nucleic Acids Res. 21: 3269-3275.
13. Liang, P. and A.B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-971.
14. Liang, P.L. and A.B. Pardee. 1995. Recent advances in differential display. Curr. Opin. Immunol. 7:274-280.
15. Raineri, I., C. Moroni and H.P. Senn. 1991. Improved efficiency for single-sided PCR by creating a reusable pool of first strand cDNA coulped to a solid phase. Nucleic Acids Res. 19:4010.
16. Rodriguez, I.R. and G.J. Chader. 1992. A novel method for the isolation of tissue-specific genes. Nucleic Acids Res. 20:3528.
17. Watt, S.M. and J.W.M. Visser. 1992. Recent advances in the growth and isolation of primitive human haematopoietic progenitor cells. Cell Prolif. 25:263-297.
18. Williams, T.M., D. Moolten, J. Burlein, J. Romano, R. Bhaerman, A. Godillot, F. Mellon, F.J. Rauscher and J.A. Kant. 1991. Identification of a zinc finger protein that inhibits IL-2 gene expression. Science 254:1791-1794.