Detection of the DRB4 null gene, DRB4*0101102N, by PCR-SSP and its distinction from other DRB4 genes

Tissue Antigens

Volumn 47, Issue 3, 1996, Pages 245-248

  O'Neill, C M ^a   Bunce, M ^a   Welsh, K I ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Allele specific amplification; DNA typing; Histocompatibility testing; HLA class II; PCR ARMS; PCR SSP; Transplantation

Indexed keywords

HLA DR ANTIGEN; PRIMER DNA;

ALLELE; ARTICLE; CONTROLLED STUDY; EXON; FEMALE; GENE ISOLATION; GENOTYPE; HUMAN; HUMAN CELL; INTRON; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; TISSUE TYPING;

EID: 0030010104     PISSN: 00012815     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-0039.1996.tb02548.x     Document Type: Article

References (17)

1. Olerup O, Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992: 39: 225-235.
2. Olerup O, Aldener A, Fogdell A. HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours. Tissue Antigens 1993: 41: 119-134.
3. Bunce M, Taylor CJ, Welsh KI. Rapid HLA-DQB typing by 8 PCR amplifications with sequence-specific primers (PCR-SSP). Hum Immunol 1993: 37: 201-206.
4. Bunce M, Welsh KI. Rapid DNA typing for HLA-C using sequence-specific primers (PCR-SSP): identification of serological and non-serologically defined HLA-Cw alleles including several new alleles. Tissue Antigens 1994: 43: 7-17.
5. Bunce M, Barnardo MCMN, Welsh KI. Improvements in HLA-C typing using sequence-specific primers (PCR-SSP) including definition of HLA-Cw9 and Cw10 and a new allele HLA-Cw"7/8v". Tissue Antigens 1994: 44: 200-203.
6. Bunce M, Fanning GC, Welsh KI. Comprehensive, serologically equivalent DNA typing for HLA-B by PCR using sequence-specific primers (PCR-SSP). Tissue Antigens 1995: 45: 81-90.
7. Bunce M, O'Neill CM, Barnardo MCNM et al. Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP). Tissue Antigens 1995: 46: 355-367.
8. Knowles RW, Flomenberg N, Horibe K, Winchester R, Radka SF, Dupont B. Complexity of the supertypic HLA-DRw53 specificity: two distinct epitopes differentially expressed on one or all of the DR β-chains depending on the HLA-DR allotype. J Immunol 1986: 137: 2618-2626.
9. Bodmer JG, Marsh SGE, Albert ED et al. Nomenclature for factors of the HLA system, 1995. Tissue Antigens 1995: 46: 1-18.
10. Sutton VR, Knowles RW. Mapping of a restriction fragment length polymorphism associated with defective DRβ4 chain expression to the HLA-DRB1 gene. Hum Immunol 1988: 22: 123-134.
11. Sutton VR, Kienzle BK, Knowles RW. An altered splice site is found in the DRB4 gene that is not expressed in HLA-DR7, Dw11 individuals. Immunogenetics 1989: 29: 317-322.
12. Mount SM. A catalogue of splice junction sequences. Nucleic Acids Res 1982: 10: 459-472.
13. Brethnach R, Chambon P. Organization and expression of eukaryotic split genes coding for proteins. Annu Rev Biochem 1981: 50: 349-383.
14. Sutton VR, Knowles RW. An aberrant DRB4 null gene transcript is found that could encode a novel HLA-DRβ chain. Immunogenetics 1990: 31: 112-117.
15. Brown JH, Jardetsky T, Saper MA, Samraoui B, Bjorkman PJ, Wiley DC. A hypothetical model of the foreign antigen binding site of Class II histocompatibility molecules. Nature 1988: 332: 845-850.
16. Marsh SGE, Bodmer JG. HLA class II region nucleotide sequences, 1995. Tissue Antigens 1995: 46: 258-80.
17. Kimura A, Ping Dong R, Harada H, Sasazuki T. DNA typing of HLA class II genes in B-lymphoblastoid cell lines homozygous for HLA. Proceedings of the 11th International Histocompatibility Workshop and Conference 1991. Oxford: Oxford University Press, 1992: volume 1: 419-425.