Cloning and analysis of DNA-binding proteins by yeast one-hybrid and one-two-hybrid systems

BioTechniques

Volumn 20, Issue 4, 1996, Pages 564-568

  Luo, Ying ^a   Vijaychander, Sharada ^a   Stile, John ^a   Zhu, Li ^a  

^a CLONTECH Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BASE PAIRING; DNA HYBRIDIZATION; GENE EXPRESSION REGULATION; GENE LOCUS; MOLECULAR CLONING; MOUSE; NONHUMAN; PROTEIN DNA BINDING; REPORTER GENE; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, FUNGAL; DNA, NEOPLASM; DNA, RECOMBINANT; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENE LIBRARY; GENETIC COMPLEMENTATION TEST; LYMPHOMA; MICE; MOLECULAR SEQUENCE DATA; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0030007247     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Alexandre, C.A., D.A. Grueneberg and M.Z. Gilman. 1993. Studying heterologous transcription factors in yeast. Methods 5:147-155.
2. Chong, J.A., J.T. Ramirez, S. Kim, J.J. Aral, Y. Zheng, M.C. Boutros, Y.M. Altshuller, M.A. Frohman, S.S. Kraner and G. Mandel. 1995. REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons. Cell 80:949-957.
3. Dalton, S. and R. Treisman. 1992. Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element. Cell 68:591-612.
4. Durfee, T., K. Becherer, P.L. Chen, S.H. Yeh, Y. Yang, A.E. Kilburn, W.H. Lee and S.J. Elledge. 1993. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Gene Dev. 7:555-569.
5. El-Deiry, W.S., S.E. Kern, J.A. Pietenpol, K.W. Kinzler and B. Vogelstein. 1992. Definition of a consensus binding site for p53. Nature Genet. 1:45-49.
6. Fields, S. and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-247.
7. Gstaiger, M., L. Knoepfel, O. Georgiev, W. Schaffner and C.M. Hovens. 1995. A B-cell coactivator of octamer-binding transcription factors. Nature 373:360-362.
8. Iwabuchi, K., B. Li, P. Bartel and S. Fields. 1993. Use of the two-hybrid system to identify the domain of p53 involved in oligomerization. Oncogene 8:1693-1696.
9. Lehming, N., D. Thanos, J.M. Brickman, J. Ma, T. Maniatis and M. Ptashne. 1994. An HMG-like protein that can switch a transcriptional activator to a repressor. Nature 371:175-179.
10. Li, J.J. and I. Herskowitz. 1993. Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. Science 262:1870-1873.
11. Lin, J., T.E. Wilson, J. Milbrandt and M. Johnston. 1993. Identifying DNA-binding sites and analyzing DNA-binding domains using a yeast selection system. Methods 5:125-137.
12. Strubin, M., J.W. Newell and P. Matthias. 1995. OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins. Cell 80:497-506.
13. Wang, M.M. and R.R. Reed. 1993. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast. Nature 364:121-126.
14. Wilson, T.E., T.J. Fahrner, M. Johnston and J. Milbrandt. 1991. A genetic method for defining DNA-binding domains: application to the nuclear receptor NGFI-B. Nature 252:1296-1300.