Characterization of rat lymphocyte primary culture for the development of an in-vitro mutagenesis assay: Effect of interleukin-2 and 2-mercaptoethanol on the activities of intermediary metabolism enzymes and cell proliferation

Cell Biology and Toxicology

Volumn 12, Issue 2, 1996, Pages 79-87

  Aidoo, A ^a   Feuers, R J ^a   Lyn Cook, L E ^a   Bishop, M E ^a   Casciano, D A ^a  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

Intermediary metabolism enzymes; Mitogen stimulation; Rat lymphocytes

Indexed keywords

ALANINE AMINOTRANSFERASE; DRUG METABOLIZING ENZYME; FATTY ACID SYNTHASE; GLUTAMATE DEHYDROGENASE; INTERLEUKIN 2; ISOCITRATE DEHYDROGENASE; LACTATE DEHYDROGENASE; MALATE DEHYDROGENASE; MERCAPTOETHANOL; PHYTOHEMAGGLUTININ; PYRUVATE KINASE;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; IN VITRO STUDY; LYMPHOCYTE ACTIVATION; LYMPHOCYTE CULTURE; MALE; MUTAGEN TESTING; NONHUMAN; PRIORITY JOURNAL; RAT;

AMINO ACIDS; ANIMALS; CELL DIVISION; CELLS, CULTURED; CITRIC ACID CYCLE; FATTY ACID SYNTHETASE COMPLEX; GLUTAMATE DEHYDROGENASE; INTERLEUKIN-2; ISOCITRATE DEHYDROGENASE; L-LACTATE DEHYDROGENASE; LYMPHOCYTE ACTIVATION; MALATE DEHYDROGENASE; MALE; MERCAPTOETHANOL; MUTAGENICITY TESTS; PHYTOHEMAGGLUTININS; PYRUVATE KINASE; PYRUVATES; PYRUVIC ACID; RATS; RATS, INBRED F344; T-LYMPHOCYTES; TRANSAMINASES;

EID: 0030006485     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00143358     Document Type: Article

References (34)

1. Aidoo A, Morris SM, Domon OE, McGarrity LJ, Kodell RL, Casciano DA. Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes. Cell Biol Toxicol. 1989;5:237-48.
2. Aidoo A, Lyn-Cook LE, Mittelstaedt RA, Heflich RH, Casciano DA. Induction of 6-thioguanine-resistant lymphocytes in Fischer 344 rats following in vivo exposure to N-ethyl-N-nitrosourea and cyclophosphamide. Environ Mol Mutagen. 1991;17:141-51.
3. Bich-Thuy TL, Dukovich M, Peffer NJ, Fauci AS, Kehrl JH, Greene WC. Direct activation of human resting T-cells by IL-2: the role of an IL-2 receptor distinct from the Tac protein. J Immunol. 1987;139:1550-6.
4. Budroe JD, Shaddock JG, Casciano DA. A study of the potential genotoxicity of methapyrilene and related antihistamines using the hepatocyte/DNA repair assay. Mutat Res. 1984;135:131-7.
5. Cheever MA, Greenberg PD, Fefer A, Gills S. Augmentation of the anti-tumor therapeutic efficacy of long-term cultured T-lymphocytes by in vivo administration of purified interleukin-2. J Exp Med. 1982;155:968-80.
6. Evans GA, Linnekin D, Grove S, Farrar WL. Specific protein kinases modulated during T-cell mitogenesis. Activity of a 55-kDa serine kinase is associated with growth arrest in human T-cells. J Biol Chem. 1992;267:10313-7.
7. Fanciulli M, Bruno T, Castinglione S, Del Carlo C, Paggi MG, Floridi A. Glucose metabolism in adriamycin-sensitive and -resistant LoVo human colon carcinoma cells. Oncol Res. 1993;5:357-62.
8. Fanger MW, Hart DA, Wells JV. Enhancement of reducing agents of the transformation of human and rabbit peripheral lymphocytes. J Immunol. 1970;105:1043-5.
9. Feuers RJ, Delongchamp RR, Casciano DA, Burkhart JG, Mohrenweiser HW. Assay for mouse tissue enzymes: levels of activity and statistical variation for 29 enzymes of liver or brain. Anal Biochem. 1980;101:123-30.
10. Fong TC, Makinodan T. Preferential enhancement by 2-mercaptoethanol of IL-2 responsiveness of T blast cells from old over young mice is associated with potentiated protein kinase C translocation. Immunol Lett. 1989;20:149-54.
11. Greer WL, Kaplan JG. Early nuclear events in lymphocyte proliferation: the role of DNA strand break repair and ADP ribosylation. Exp Cell Res. 1986;166:399-415.
12. Guppy M, Griner E, Brand K. The role of the Crabtree effect and an endogenous fuel in the energy metabolism of resting and proliferating thymocytes. Eur J Biochem. 1993;212:95-9.
13. Haseman JK, Crawford DD, Huff JE, Boorman GA, McConnell EE. Results from 86 two-year carcinogenicity studies conducted by the National Toxicology Program. J Toxicol Environ Health. 1984;14:621-39.
14. Helderman JH, Womble D, Chandour E, Womble S. Identification of a monocyte-derived factor which regulates synthesis of insulin receptors on activated T-lymphocytes (MIRRF). Cytokine. 1992;4:528-36.
15. Jones IM, Burkhart-Shultz K, Strout CL, Crippen TL. Factors that affect the frequency of thioguanine-resistant lymphocytes in mice following exposure to ethylnitrosourea. Environ Mutagen. 1987;9:317-29.
16. Koffler R, Raskin P, Womble D, Helderman JH. Immunological consequences of regulation of the insulin receptor on alloactivated lymphocytes in normal and obese subjects. Diabetes. 1991;40:364-70.
17. Loeb LA, Agarwal SS, Woodside AM. Induction of DNA polymerase in human lymphocytes by phytohemagglutinin. Proc Natl Acad Sci USA. 1968;61:827-34.
18. Loeb LA, Ewald JL, Agarwal SS. DNA polymerase and DNA replication during lymphocyte transformation. Cancer Res. 1970;30:2514-20.
19. McNerney R, Tavasolli M, Shall S, Brazinski A, Johstone A. Changes in mRNA levels of poly(ADP-ribose) polymerase during activation of human lymphocytes. Biochem Biophys Acta. 1989;1009:185-7.
20. Moore JP, Tood JA, Hesketh TR, Metcalfe JC. c-fos and c-myc Gene activation, ionic signals and DNA synthesis in thymocytes. J Biol Chem. 1986;261:8158-62.
21. Morris SM, Aidoo A, Domon OE et al. Effect of interleukin-2 on cell proliferation, sister-chromatid exchange induction, and nuclear stress protein phosphorylation in PHA-stimu-lated Fischer 344 rat spleen lymphocytes: modulation by 2-mercaptoethanol. Environ Mol Mutagen. 1990;15:10-8.
22. Noelle RJ, Lawrence DA. Modulation of T-cell functions. I. Effect of 2-mercaptoethanol and macrophages on T-cell proliferation. Cell Immunol. 1980;50:416-31.
23. Ochoa AC, Gromo G, Alter BJ, Sondel PM, Bach FH. Long-term growth of lymphocyte-activated killer (LAK) cells: role of anti-CD3, β-IL-1, interferon-γ and -β1. J Immunol. 1987;138:2728-33.
24. Pesquero J, Albi JL, Gallardo MA, Planas J, Sanchez J. Glucose metabolism by trout (Salmo trutta) red blood cells. J Comp Phys. 1992;162:448-54.
25. Rabinowitz Y, Wong P, Wilhite BA. Effect of phytohemagglutinin on enzymes of thymidine salvage pathway of cultured chronic lymphatic leukemic lymphocytes. Blood. 1970;35: 236-42.
26. Rosenberg SA, Lotze MT, Muul LM et al. Observations on the systemic administration of autologous lymphokine-activated killer cells and recombinant interleukin-2 to patients with metastatic cancer. N Engl J Med. 1985;313:1485-92.
27. Sakaguchi AY, Sylvia VL, Martinez L, Smith EA, Han ES. Assignment of tyrosine-specific T-cell phosphatase to conserved syntenic groups on human chromosome 18 and mouse chromosome 18. Genomics. 1992;12:151-4.
28. Schacterle GR, Pollack RL. A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem. 1973;51:654-5.
29. Stobo JD, Rosenthal AS, Paul WE. Functional heterogeneity of murine lymphoid cell. I. Responsiveness to and surface binding of concanavalin A and phytohemagglutinin. J Immunol. 1972;108:1-17.
30. Strauss GH, Albertini RH. Enumeration of 6-thioguanine-resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivo. Mutat Res. 1979;61:353-79.
31. Tennant RW, Margolin BH, Shelby MD et al. Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays. Science. 1987;236:933-41.
32. Van Dam FJ, Natarajan AT, Tates AD. Use of a T-lymphocyte clonal assay for determining HPRT mutant frequencies in individual rats. Mutation Res. 1992;271:231-42.
33. Whitesell RR, Aboumrad MK, Powers AC et al. Coupling of glucose transport and phosphorylation in Xenopus oocytes and cultured cells: determination of the rate-limiting step. J Cell Physiol. 1993;157:509-18.
34. Zimmer DM, Aaron CS, O'Neill JP, Albertini RJ. Enumeration of 6-thioguanine-resistant T-lymphocytes in the peripheral blood of nonhuman primates. (Cynomolgus monkeys). Environ Mol Mutagen. 1991;18:161-7.