Pub1 acts as an E6-AP-like protein ubiquitin ligase in the degradation of cdc25

EMBO Journal

Volumn 15, Issue 6, 1996, Pages 1301-1312

  Nefsky, Bradley ^a   Beach, David ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

cdc25; Cell cycle; Protein degradation; S.pombe; Ubiquitination

Indexed keywords

PHOSPHATASE; THIOESTER; UBIQUITIN; UBIQUITIN PROTEIN LIGASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; MITOSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY;

SCHIZOSACCHAROMYCES POMBE;

EID: 0030006130     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00472.x     Document Type: Article

References (78)

1. Amon, A., Irniger, S. and Nasmyth, K. (1994) Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle. Cell, 77, 1037-1050.
2. Ausubel, F.M., Brent , R., Kingston, R.E., Moore, D.D., Seidman, J.G , Smith, J.A. and Struhl, K. (1991) Current Protocols in Molecular Biology. Greene/John Wiley, New York.
3. Bartel, B., Wunning, J. and Varshavsky, A. (1990) The recognition component of the N-end rule pathway. EMBO J., 9, 3179-3189.
4. + controls the transition from mitotic division to meiosis in fission yeast. Nature, 300, 706-709
5. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 72, 248-254
6. Braun, S., Raymond, W.E. and Racker, E. (1984) Synthetic tyrosine polymers as substrates and inhibitors of tyrosine-specific protein kinases. J. Biol. Chem., 259, 2051-2054.
7. Casnelli, J. (1991) Assay of protein kinases using peptides with basic residues for phosphocellulose binding. Methods Enzymol., 200, 115-120.
8. Chen, P., Johnson, P., Sommer, T., Jentsch, S. and Hochstrasser, M. (1993) Multiple ubiquitin conjugating enzymes participate in the in vivo degradation of the yeast MAT α2 repressor. Cell, 74, 357-369.
9. Ciechanover, A. (1994) The ubiquitin-proteasome proteolytic pathway Cell, 79, 13-21.
10. +2-dependent translocation domain with homology to PKC and GAP. Cell, 65, 1043-1051.
11. Crook, T., Wrede, D and Vousden, K H. (1991) p53 point mutation in HPV negative human cervical carcinoma cell lines. Oncogene, 6, 873-875.
12. Dohmen, R.J., Madura, K., Bartel, B. and Varshavsky, A. (1991) The N-end rule is mediated by the UBC2 (RAD6) ubiquitin-conjugating enzyme. Proc. Natl Acad. Sci. USA, 88, 7351-7355
13. Druker, B J., Mamon, H.J. and Roberts, T.M. (1989) Oncogenes, growth factors and signal transduction. New Engl. J. Med., 321, 1383-1391.
14. Ducommun, B , Draetta, G., Young, P. and Beach, D (1990) Fission yeast cdc25 is a cell cycle regulated protein. Biochem. Biophys. Res. Commun., 167, 301-309.
15. Dunphy, W.G. and Kumagai, A. (1991) The cdc25 protein contains an intrinsic phosphatase activity. Cell, 67, 189-196.
16. Fantes, P. (1979) Epistatic gene interactions in the control of division in fission yeast. Nature, 279, 428-430.
17. wee1 mitotic inhibitor is a tyrosine/serine kinase. Nature, 349, 808-811.
18. Fesquet, D. et al. (1993) The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of thr 161 and its homologues. EMBO J., 12, 3111-3121.
19. Finley, D. and Chau, V. (1991) Ubiquitination. Annu Rev. Cell Biol., 7, 25-69.
20. Forsburg, S and Nurse, P. (1991) Cell cycle regulation in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Annu. Rev. Cell Biol., 7, 227-256.
21. Foster, R., Thorner, J. and Martin, G.S. (1989) Nucleotidylation, not phosphorylation, is the major source of the phosphotyrosine detected in enteric bacteria J. Bacteriol., 171, 272-279.
22. Galaktionov, K , Jessus, C. and Beach, D. (1995a) Raf1 interaction with cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev., 9, 1046-1058.
23. Galaktionov, K., Lee, A.K., Eckstein, J., Draetta, G., Meckler, J., Loda, M. and Beach, D. (1995b) Cdc25 phosphatases as potential human oncogenes. Science, 269, 1575-1577.
24. cdc2. Cell, 67, 197-211.
25. Glotzer, M., Murray, A.W. and Kirschner, M.W. (1991) Cyclin is degraded by the ubiquitin pathway. Nature, 349, 132-138.
26. Gordon, C., McGurk, G., Dillon, P , Rosen, C. and Hastie, N.D. (1993) Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit. Nature, 366, 355-357.
27. + protein kinase regulates entry into mitosis. Nature, 342, 39-45
28. cdc2 function. EMBO J., 10, 3297-3309.
29. Gutz, H., Heslot, H , Leupold, U. and Loprieno, N. (1974) Schizosaccharomyces pombe. In King, R.C. (ed.), Handbook of Genetics. Plenum Press, New York, Vol. 1, pp. 395-446.
30. Haas, A.L., Murphy, K E. and Bright, P.M. (1985) The inactivation of ubiquitin accounts for the inability to demonstrate ATP, ubiquitin-dependent proteolysis in liver extracts. J. Biol Chem., 260, 4694-4703.
31. Hanks, S.K. and Quinn, A.M. (1991) Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members. Methods Enzymol., 200, 38-62.
32. Heald, R., McLoughlin, M. and McKeon, F. (1993) Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell, 74, 463-174.
33. Henikoff, S. (1987) Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods Enzymol., 155, 156-165.
34. Hershko, A., Eytan, E., Ciechanover, A. and Haas, A (1982) Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. J. Biol. Chem., 257, 13964-13970.
35. Holloway, S.L., Glotzer, M., King, R.W. and Murray, A.W. (1993) Anaphase is initiated by proteolysis rather than by the inactivation of maturation promoting factor. Cell, 73, 1393-1402.
36. Huibregtse, J.M., Scheffner, M. and Howley, P.M. (1991) A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18. EMBO J., 13, 4129-4135
37. Huibregtse, J.M., Scheffner, M. and Howley, P.M. (1993a) Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53. Mol. Cell. Biol., 13, 775-784.
38. Huibregtse, J.M., Scheffner, M. and Howley, P.M. (1993b) Localization of the E6-AP regions that direct human papillomavirus E6 binding, association with p53 and ubiquitination of associated proteins. Mol. Cell. Biol., 13, 4918-4927.
39. Huibregtse, J M., Scheffner, M., Beaudenon, S. and Howley, P M. (1995) A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl Acad Sci. USA, 92, 2563-2567.
40. Kimura, K., Nakano, Y. and Matsuoka, K. (1989) O-phosphotyrosyl glutamine synthetase: modification of the nucleotide ligation site of adenylylated glutamine synthetase. J. Biochem. Tokyo, 105, 84-87.
41. King, R.W., Jackson, P K and Kirscher, M.W. (1994) Mitosis in transition. Cell, 79, 563-571.
42. Kumar, S., Tomooka, Y. and Noda, M. (1992) Identification of a set of genes with developmentally down regulated expression in the mouse brain. Biochem. Biophys. Res. Commun., 185, 1155-1161.
43. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 277, 680-685.
44. Leupold, U. (1970) Genetical methods for Schizosaccharomyces pombe, Methods Cell Physiol., 4, 169-177.
45. Lindberg, R.A. and Pasquale, E.B. (1991) Isolation of cDNA clones that encode active protein tyrosine kinases using antibodies against phosphotyrosine. Methods Enzymol., 200, 557-564.
46. Lindberg, R.A., Thompson, D.P. and Hunter, T. (1988) Identification of cDNA clones that code for protein tyrosine kinases by screening expression libraries with antibodies against phosphotyrosine. Oncogene, 3, 629-633.
47. Lundgren, K., Walworth, N., Booher, R., Dembski, M., Kirschner, M. and Beach, D. (1991) Mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2. Cell, 64, 1111-1122.
48. Madura, K. and Varshavsky, A (1994) Degradation of Gα by the N-end rule pathway. Science, 265, 1454-1458.
49. Maudrell, K. (1993) Thiamin repressible expression vectors pREP and pRIP for fission yeast. Gene, 123, 127-130.
50. McGrath, J.P., Jentsch, S. and Varshavsky, A. (1991) UBA1: an essential yeast gene encoding ubiquitin-activating enzyme. EMBO J., 10, 227-236.
51. McKinney, J.D., Chang, F. Heintz, N and Cross, F (1993) Negative regulation of FAR1 at the start of the yeast cell cycle. Genes Dev., 7, 833-843.
52. Millar, J.B A., Lenaers, G. and Russell, P. (1992) Pyp3 PTPase acts as a mitotic inducer in fission yeast. EMBO J., 11, 4933-4941
53. Mizukami, T. et al. (1993) A 13kb resolution cosmid map of the 14Mb fission yeast genome by nonrandom sequence-tagged site mapping. Cell, 73, 121-132.
54. cdc25 mitotic inducer in fission yeast. Nature, 344, 549-552.
55. Moreno, S , Klar, A. and Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol., 194, 795-823.
56. Morgan, D. (1995) Principles of CDK regulation. Nature, 374, 131-134.
57. Morla, A O., Draetta, G., Beach, D. and Wang, J. (1989) Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. Cell, 58, 193-203.
58. Murray, A.W., Solomon, M.J. and Kirschner, M.W. The role of cyclin synthesis and degradation in the control of maturation promoting factor activity. (1989) Nature, 393, 280-286.
59. Norbury, C. and Nurse, P. (1992) Animal cell cycles and their control. Annu. Rev Biochem., 61, 441-470.
60. 2 production in the fission yeast Schizosaccharomyces pombe persist after a block to protein synthesis. J. Cell Sci., 87, 323-325.
61. Nurse, P. (1975) Genetic control of cell size at cell division in yeast. Nature, 256, 547-551.
62. Nurse, P. and Thuriaux, P. (1980) Regulatory genes controlling mitosis in fission yeast Schizosaccharomyces pombe. Genetics, 96, 627-637.
63. cdc2 on tyrosine 15. Proc. Natl Acad. Sci USA, 89, 2917-2921.
64. Racker, E. (1991) Use of synthetic amino acid polymers for assay of protein tyrosine and protein serine kinases. Methods Enymol., 200, 107-111.
65. + functions as an inducer of mitotic control of fission yeast. Cell, 45, 145-153.
66. +, a gene encoding a protein kinase homolog. Cell, 49, 559-567.
67. Sambrook, J., Fritsch, H.F. and Maniatis, T (1989) Molecular Cloning. A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
68. Scheffner, M., Munger, K., Byrne, J.C. and Howley, P.M. (1991) The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl Acad. Sci. USA, 88, 5523-5527.
69. Scheffner, M., Huibregtse, J.M., Viersta, R.D. and Howley, P.M. (1993) The HPV-16 E6 and E6-AP complex functions as a ubiquitin protein ligase in the ubiquitination of p53. Cell, 75, 495-505
70. Scheffner, M., Nuber, U. and Huibretse, J.M. (1995) Protein ubiquitination involving an E1-E2-E3 enzyme ubiquitin thioester cascade. Nature, 373, 81-83.
71. Seufert, W., Futcher, B. and Jentsch, S. (1995) Role of a ubiquitin-conjugating enzyme in degradation of S and M phase cyclins. Nature, 373, 78-81.
72. SIC1 controls the G1 to S transition in S.cerevisiae. Cell, 79, 233-244.
73. cdc2 activation, identification of an activating kinase. Mol. Biol. Cell, 3, 13-27.
74. Swerdlow, P.S., Finley, D and Varshavsky, A. (1986) Enhancement of immunoblot sensitivity by heating of hydrated filters. Anal. Biochem., 156, 147-153.
75. Varshavsky, A. (1992) The N-end rule. Cell, 69, 725-735
76. Viestra, R.D., Langan, S.M. and Haas, A.L (1985) Purification and initial characterization of ubiquitin from the higher plant avena sativa. J. Biol. Chem., 260, 12015-12021.
77. Wong, T.W. and Goldberg, A R. (1983) In vitro phosphorylation of angiotensin analogues by tyrosyl protein kinases. J. Biol. Chem., 258, 1022-1025
78. Wrede, D., Tidy, J.A., Crook, T., Lane, D and Vousden, K.H. (1991) Expression of RB and p53 proteins in HPV-positive and HPV-negative cervical carcinoma cell lines. Mol. Carcinogen., 4, 171-175.