Analysis of the mechanism of activation of cAMP-dependent protein kinase through the study of mutants of the yeast regulatory subunit

European Journal of Biochemistry

Volumn 237, Issue 1, 1996, Pages 136-142

  Zaremberg, Vanina ^a   Moreno, Silvia ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

cAMP phosphodiesterase; Protein kinase A; RAS2; Regulatory subunit; Yeast

Indexed keywords

CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVERTANT; YEAST;

3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENZYME ACTIVATION; MUTATION; PHENOTYPE; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0030005951     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0136n.x     Document Type: Article

References (31)

1. Toda, T., Cameron, S., Sass, P., Zoller, M., Scott, J. D., McMullen, B., Hurwitz, M., Krebs, E. G. & Wigler, M. (1987) Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae, Mol. Cell. Biol. 7, 1371-1377.
2. Cannon, J. F. & Tatchell, K. (1987) Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase, Mol. Cell. Biol. 7, 2653-2663.
3. Thevelein, J. M. (1994) Signal transduction in yeast, Yeast 10, 1753-1790.
4. Tatchell, K. (1986) RAS genes and growth control in Saccharomyces cerevisiae, J. Bacteriol. 166, 364-367.
5. Thevelein, J. M. (1992) The RAS-adenylate cyclase pathway and cell cycle control in Saccharomyces cerevisiae, Antonie Leeuwenhoek 62, 109-130.
6. Cannon, J. F., Gibbs, J. B. & Tatchell, K. (1986) Suppressors of the ras2 mutation of Saccharomyces cerevisiae, Genetics 113, 247-264.
7. Cannon, J. F., Gitan, R. & Tatchell, K. (1990) Yeast cAMP-dependent protein kinase regulatory subunit mutations display a variety of phenotypes, J. Biol. Chem. 265, 11 897-11 904.
8. Sherman, F., Fink, G. & Hicks, J. B. (1981) Methods in yeast genetics: a laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
9. Christianson, T., Sikorski, R. S., Dante, M., Shero, J. H. & Hieter, P. (1992) Multifunctional yeast high-copy-number shuttle vectors, Gene (Amst.) 110, 119-122.
10. m cyclic AMP phosphodiesterase of Saccharomyces cerevisiae, Mol. Cell. Biol. 8, 505-510.
11. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations, J. Bacteriol. 153, 163-168.
12. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
13. Maggese, M. C., Galvagno, M. A., Cantore, M. L. & Passeron, S. (1982) In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii, Cell Biol. Int. Rep. 6, 1101-1108.
14. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193, 265-275.
15. Ekanger, R., Vintermyr, O., Houget, G., Sand, T., Scott, J., Krebs, E. G., Eikhom, T., Christoffersen, T., Øgreid, D. & Døskeland, S. (1989) The expression of cAMP-dependent protein kinase subunits is differentially regulated during liver regeneration, J. Biol. Chem. 264, 4374-4382.
16. Moreno, S., Pastori, R. & Passeron, S. (1983) Protein kinase of Mucor rouxii. Unshielding of new cyclic AMP-binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP, Mol. Cell. Biochem. 52, 13-16.
17. Mueller, G. & Bandlow, W. (1991) Two lipid-anchored cAMP-binding proteins in the yeast Saccharomyces cerevisiae are unrelated to the R subunit of cytoplasmic protein kinase A, Eur. J. Biochem. 202, 299-308.
18. Roedel, G., Mueller, G. & Bandlow, W. (1985) Cyclic AMP receptor protein from yeast Saccharomyces cerevisiae mitochondria: submitochondrial localization and preliminary characterization, J. Bacteriol. 161, 7-12.
19. Roskoski, R. (1983) Assay of protein kinase, Methods Enzymol. 99, 3-6.
20. Thompson, W. J., Brooker, G. & Appleman, M. M. (1974) Assay of cyclic nucleotide phosphodiesterase with radioactive substrates, Methods Enzymol. 38, 205-217.
21. Kuret, J., Johnson, K., Nicolette, C. & Zoller, M. J. (1988), Mutagenesis of the regulatory subunit of yeast cyclic AMP-dependent protein kinase; isolation of site-directed mutants with altered binding affinity for catalytic subunit, J. Biol. Chem. 263, 9149-9154.
22. Døskeland, S. O., Maronde, E. & Gjertsen B. T. (1993) The genetic subtypes of cAMP-dependent protein kinase: functionally different or redundant? Biochem. Biophys. Acta 1178, 249-258.
23. McKnight, G. S. (1991) Cyclic nucleotide dependent protein kinases, Curr. Opin. Cell Biol. 3, 213-217.
24. Kessler, R., Gartner, G., Schellenberger, W. & Hofmann, E. (1991) Fructose-2,6-bisphosphate metabolism in permeabilized yeast cells, Biomed. Biochim. Acta 50, 851-860
25. Matsumoto, K., Uno, Y., Oshima, Y. & Ishikawa, T. (1982) Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase, Proc. Natl Acad. Sci. USA 79, 2355-2359.
26. Yang, S., Fletcher, W. H. & Johnson, D. A. (1995) Regulation of cAMP-dependent protein kinase: enzyme activation without dissociation, Biochemistry 34, 6267-6271.
27. Dekker, L. V. & Parker, P. S. (1994) Protein kinase C; a question of specificity, Trends Biochem. Sci. 19, 73-77.
28. Scott, J. D. (1991) Cyclic nucleotide dependent protein kinases, Pharmacol. & Ther. 50, 123-145.
29. Tripp, M. L., Piñón, R., Meisenhelder, J. & Hunter, T. (1986) Identification of phosphoproteins correlated with proliferation and cell cycle arrest in Saccharomyces cerevisiae: positive and negative regulation of cAMP-dependent protein kinase, Proc. Natl Acad. Sci. USA 38, 5973-5977.
30. Guthmann, M., Pastori, R. & Moreno, S. (1990) Polyamines and basic proteins stimulate activation by cAMP and catalytic activity of Mucor rouxii cAMP dependent protein kinase, Cell. Signalling 2, 395-402.
31. Beavo, J. A., Bechtel, P. J. & Krebs, E. G. (1974) Activation of protein kinase by physiological concentrations of cAMP, Proc. Natl. Acad. Sci. USA 71, 3580-3583.