Fate of germ cells in 2,5-hexanedione-induced testicular injury I. Apoptosis is the mechanism of germ cell death

Toxicology and Applied Pharmacology

Volumn 137, Issue 2, 1996, Pages 141-148

  Blanchard, Kerry T ^a   Allard, Elizabeth K ^a   Boekelheide, Kim ^a  

^a BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA;

ACETONYLACETONE; DNA FRAGMENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; ETIOLOGY; GERM CELL; HISTOPATHOLOGY; MALE; NONHUMAN; RAT; SPERMATOCYTE; SPERMATOGONIUM; TESTIS INJURY;

ANIMALS; APOPTOSIS; ATROPHY; CELL DEATH; DIGOXIGENIN; DNA; DNA NUCLEOTIDYLEXOTRANSFERASE; ELECTROPHORESIS, AGAR GEL; HEXANONES; MALE; RATS; RATS, INBRED F344; SPERMATOZOA; STAINING AND LABELING; TESTIS; URIDINE TRIPHOSPHATE;

EID: 0030005783     PISSN: 0041008X     EISSN: None     Source Type: Journal    
DOI: 10.1006/taap.1996.0066     Document Type: Article

References (42)

1. Allard, E. K., Johnson, K. J., Boekelheide, K. (1993). Colchicine disrupts the cytoskeleton of rat testis seminiferous epithelium in a stage-dependent manner. Biol. Reprod. 48, 143-153.
2. Allard, E. K., Hall, S. J., and Boekelheide, K. (1995). Stem cell kinetics in rat testis after irreversible injury induced by 2,5-hexanedione. Biol. Reprod. 53, 186-192.
3. Allard, E. K., and Boekelheide, K. (1996). Fate of germ cells in 2,5-hexanedione-induced testicular injury. II. Atrophy persists due to a reduced stem cell mass and ongoing apoptosis. Toxicol. Appl. Pharmacol. 137, 149-156.
4. Allen, D. J., Harmon, B. V., and Kerr, J. F. R. (1987). Cell death in spermatogenesis. In Prospectives on Mamalian Cell Death (C. S. Potten, Ed.). Oxford Univ. Press, New York.
5. Andrews, L. S., and Snyder, R. (1991). Toxic effects of solvents and vapors. In Casarett and Doull's Toxicology, the Basic Science of Poisons (M. O. Amdur, J. Doull, and C. D. Klaassen, Eds.), pp. 681-722. Pergamon Press, New York.
6. Arends, M. J., Morris, R. G., and Wyllie, A. H. (1990). Apoptosis: The role of endonuclease. Am. J. Pathol. 136(3), 593-608.
7. Auger, J., Kunstmann, J. M., Czyglik, F., and Jouanet, P. (1995). Decline in semen quality among fertile men in Paris during the past 20 years. N. Engl. J. Med. 332, 281-285.
8. Boekelheide, K. (1987a). 2,5-Hexanedione alters microtubule assembly. I. Testicular atrophy, not nervous system toxicity, correlates with enhanced tubulin polymerization. Toxicol. Appl. Pharmacol. 88, 370-382.
9. Boekelheide, K. (1987b). 2,5-Hexanedione alters microtubule assembly. II. Enhanced polymerization of crosslinked tubulin. Toxicol. Appl. Pharmacol. 88, 383-396.
10. Boekelheide, K. (1988a). Rat testis during 2,5-hexanedione intoxication and recovery. I. Dose response and the reversibility of germ cell loss. Toxicol. Appl. Pharmacol. 92, 18-27.
11. Boekelheide, K. (1988b). Rat testis during 2,5-hexanedione intoxication and recovery. II. Dynamics of pyrrole reactivity, tubulin content, and microtubule assembly. Toxicol. Appl. Pharmacol. 92, 28-33.
12. Boekelheide, K., and Hall, S. J. (1991). 2,5-Hexanedione exposure in the rat results in long-term testicular atrophy despite the presence of residual spermatogonia. J. Androl. 12, 18-26.
13. Boekelheide, K., Eveleth, J., Neely, M. D., and Sioussat, T. M. (1991). Microtubule assembly is altered following covalent modification by the n-hexane metabolite 2,5-hexanedione. Adv. Exp. Med. Biol. 283, 433-442.
14. Bursch, W., Kleine, L., and Tenniswood, M. (1990a). Biochemistry of cell death by apoptosis. Biochem. Cell Biol. 68, 1071-1074.
15. Bursch, W., Paffe, S., Putz, B., Barthel, G., and Schulte-Hermann, R. (1990b). Determination of the length of the histological stages of apoptosis in normal liver and altered hepatic foci of rats. Carcinogenesis 11, 847-853.
16. Carlsen, E., Giwercman, A., ZKeiding, N., and Skakkebaek, N. E. (1992). Evidence for decreasing quality of semen during the past 50 years. Br. J. Med. 305, 609-613.
17. Chapin, R. E., Morgan, K. T., and Bus, J. S. (1983). The morphogenesis of testicular degeneration induced in rats by orally administered 2,5-hexanedione. Exp. Mol. Pathol. 38, 149-169.
18. Collins, R. J., Harmon, B. V., Gobe', G. C., and Kerr, J. F. R. (1992). Internucleosomal DNA cleavage should not be the sole criterion of identifying apoptosis. Int. J. Rad. Biol. 61, 451-453.
19. Corcoran, G. B., Fix, L., Jones, D. P., Moslen, M. T., Nicotera, P., Oberhammer, F. A., and Buttyan, R. (1994). Contemporary issues in toxicology. Apoptosis: molecular control point in toxicology. Toxicol. Appl. Pharmacol. 128, 169-181.
20. Giwercman, A., Carlsen, E., Keiding, N., and Skakkebaek, N. E. (1993). Evidence for increasing incidence of abnormalities of the human testis: A review. Environ. Health Perspect. 101(Suppl. 2), 65-71.
21. Herskowitz, A., Ishii, H., and Schaumburg, H. H. (1971). n-Hexane neuropathy: A syndrome occurring as a result of industrial exposure. N. Engl. J. Med. 285, 82-85.
22. Huckins, C. (1978). The morphology and kinetics of spermatogonial degeneration in normal adult rats: An analysis using a simplified classification of the germinal epithelium. Anat. Rec. 190, 905-926.
23. Irvine, D. S. (1994). Falling sperm quality. Br. Med. J. 309, 476.
24. Johnson, K. J., Hall, E. S., and Boekelheide, K. (1991). 2,5-Hexanedione exposure alters the rat Sertoli cell cytoskeleton. I. Microtubules and seminiferous tubule fluid secretion. Toxicol. Appl. Pharmacol. 111, 432-442.
25. Kerr, J. B. (1992). Spontaneous degeneration of germ cells in rat testis: Assessment of cell types and frequency during the spermatogenic cycle. J. Reprod. Fertil. 95, 825-830.
26. Krasavage, W. J., O'Donogue, J. L., DiVincenzo, G. D., and Terhaar, C. J. (1980). The relative neurotoxicity of methyl-n-butyl ketone, n-hexane, and their metabolites. Toxicol. Appl. Pharmacol. 52, 433-441.
27. Majno, G., and Joris, I. (1995). Apoptosis, oncosis, and necrosis: An overview of cell death. Am. J. Pathol. 146(1), 3-15.
28. Meistrich, M. L., Reid, B. O., and Barcellona, W. J. (1976). Changes in sperm nuclei during spermiogenesis and epididymal maturation. Exp. Cell Res. 99, 72-78.
29. O'Donoghue, J. L., Krasavage, W. J., and Terhaar, C. J. (1978). Toxic effects of 2,5-hexanedione. Toxicol. Appl. Pharmacol. 45, 269.
30. Perbellini, L., Brugnone, F., and Pavan, I. (1980). Identification of the metabolites of n-hexane, cyclohexane, and their isomers in men's urine. Toxicol. Appl. Pharmacol. 53, 220-229.
31. Richburg, J. H., Redenbach, D. M., and Boekelheide, K. (1994). Seminiferous tubule fluid secretion is a Sertoli cell microtubule-dependent process inhibited by 2,5-hexanedione exposure. Toxicol. Appl. Pharmacol. 128, 302-309.
32. Russell, L. D. (1993). Role in spermiation. In The Sertoli Cell (L. D. Russell and M. D. Griswold, Eds.). Cache River Press, Clearwater, FL.
33. Schmitz, G. G., Walter, T., Seibl, R., and Kessler, C. (1991). Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. Anal. Biochem. 192, 222-231.
34. Shikone, T., Billing, H., and Hsueh, A. J. W. (1994). Experimentally induced cryptorchidism increases apoptosis in rat testis. Biol. Reprod. 51, 856-872.
35. Spencer, P. S., Shaumberg, H. H., Sabri, M. I., and Veronesi, B. (1980). The enlarging view of hexacarbon neurotoxicity. Crit. Rev. Toxicol. 7, 279-356.
36. Strauss, W. M. (1994). Preparation of genomic DNA from mammalian tissue. In Current Protocols in Molecular Biology (F. M. Ausubel et al., Eds.), Unit 2.2, pp. 2.2.1-2.2.3. Willey, New York.
37. Tapanainen, J. S., Tilly, J. L., Vhiko, K. K., and Hsueh, A. J. W. (1993). Hormonal control of apoptotic cell death in the testis: Gonadotropins and androgens as testicular cell survival factors. Mol. Endocrinol. 7(5), 643-650.
38. Troiano, L., Fustini, F., Lovato, E., Frasoldati, A., Malorni, W., Capri, M., Grassilli, E., Marrama, P., and Franceschi, C. (1994). Apoptosis and spermatogenesis: Evidence from an in vivo model of testosterone withdrawal in the adult rat. Biochem. Biophys. Res. Commun. 202(3), 1315-1321.
39. Weber, K., and Osborn, M. (1969). The reliability of molecular weight determinations by dodecyl sulfate - polyacrylamide gel electrophoresis. J. Biol. Chem. 244(16), 4406-4412.
40. Williams, G. T., and Smith, C. A. (1993). Molecular regulation of apoptosis: Genetic controls on cell death. Cell 74, 777-779.
41. Zar, J. H. (1984). Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, NJ.
42. Zirkin, B. R. (1993). Regulation of spermatogenesis in the adult mammal: Gonadotropins and androgens. In Cell and Molecular Biology of the Testis (C. Desjardins and L. L. Ewing, Eds.). Oxford Univ. Press, New York.