Immunohistochemical localization of the estrogen receptor in human osteoblastic SaOS-2 cells: Association of receptor levels with alkaline phosphatase activity

Bone

Volumn 18, Issue 4, 1996, Pages 361-369

  Sutherland, M K ^a   Hui, D U ^a   Rao, L G ^a   Wylie, J N ^a   Murray, T M ^a  

^a ST MICHAEL'S HOSPITAL   (Canada)

Author keywords

1,25 dihydroxyvitamin D3; Alkaline phosphatase; Estrogen; Estrogen receptor; Monoclonal antibody; Osteoblasts

Indexed keywords

17ALPHA ESTRADIOL; ALKALINE PHOSPHATASE; CALCITRIOL; ESTROGEN; ESTROGEN RECEPTOR; MESSENGER RNA; MONOCLONAL ANTIBODY; RECEPTOR PROTEIN; TAMOXIFEN;

ARTICLE; CELL CULTURE; CELL NUCLEUS; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOSOL; DOSE RESPONSE; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; NORTHERN BLOTTING; OSTEOBLAST; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; VARIANCE;

EID: 0030005668     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/8756-3282(96)00016-6     Document Type: Article

References (38)

1. Avioli, L. V. Hormonal alterations and osteoporotic syndromes. J Bone Miner Res 8 (Suppl. 2):S511-S514; 1993.
2. 3. Endocrinology 133:553-562; 1993.
3. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248-254; 1976.
4. Colston, K. W., King, R. J. B., Hayward, J., Fraser, D. I., Horton, M. A., Stevenson, J. C., and Arnett, T. R. Estrogen receptors and human bone cells: Immunocytochemical studies. J Bone Miner Res 4:625-631; 1989.
5. Chomczynski, P., and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159; 1987.
6. Davis, V. L., Couse, J. F., Gray, T. K., and Korach, K. S. Correlation of low estrogen receptor levels and estrogen responsiveness in 2 rat osteoblast-like cell lines. J Bone Miner Res 9:983-991; 1994.
7. 3 on cultured osteoblasts from mature rats. Bone Miner 11:273-283; 1990.
8. Enksen, E. F., Colvard, D. S., Berg, N. J., Graham, M. L., Mann, K. G., Spelsberg, T. C., and Riggs, B. L. Evidence of estrogen receptors in normal human osteoblast-like cells. Science 241:84-86; 1988.
9. Ernst, M., Heath, J. K., and Rodan, G. A. Estradiol effects on proliferation, messenger ribonucleic acid for collagen and insulin-like growth factor I, and parathyroid hormone-stimulated adenylate cyclase activity in osteoblastic cells from calvariae and long bones. Endocrinology 125:825-833; 1989.
10. Etienne, M. C., Fischel, J. L., Milano, G., Formento, P., Formento, J. L., Francoual, M., Frenay, M., and Namer, M. Steroid receptors in human osteoblastlike cells. Eur J Cancer 7:807-810; 1990.
11. Farley, J. R., Hall, S. L., Herring, S., Tarbaux, N. M., Matsuyama, T., and Wergedal, J. E. Skeletal alkaline phosphatase specific activity is an index of osteoblast phenotype in subpopulations of the human osteosarcoma cell line SaOS-2. Metabolism 40:664-671; 1991.
12. Fukayama, S. and Tashjian, A. H. Direct modulation by estradiol of the response of human bone cells (SaOS-2) to human parathyroid hormone (PTH) and PTH-related protein. Endocrinology 124:397-401; 1989.
13. Henderson, C. J. and Wolf, C. R. Immunodetection of proteins by Western blotting. In: Methods in Molecular Biology: Immunochemical Protocols. Vol. 10. Manson, M., Ed. Totowa, NY: Humana; 1992; 221-233.
14. Horigome, T., Ogata, F., Golding, T. S., and Korach, K. S. Estradiol-stimulated proteolytic cleavage of the estrogen receptor in mouse uterus. Endocrinology 123:2540-2548; 1988.
15. Huo, B., Dossing, D. A., and Dimuzio, M. T. Generation and characterization of a human osteosarcoma cell line stably transfected with the human estrogen receptor gene. J Bone Miner Res 10:769-781; 1995.
16. Ikegami, A., Inoue, S., Hosoi, T., Kaneki, M., Mizuno, Y., Akeda, Y., Ouchi, Y., and Orimo, H. Cell cycle-dependent expression of estrogen receptor and effect of estrogen on proliferation of synchronized human osteoblast-like osteosarcoma cells. Endocrinology 135:782-789; 1994.
17. Ikegami, A., Inoue, S., Hosoi, T., Mizuno, Y., Nakamura, T., Ouchi, Y., and Orimo, H. Immunohistochemical detection and northern blot analysis of estrogen receptor in osteoblastic cells. J Bone Miner Res 8:1103-1109: 1993.
18. Kaye, A. M., Weisman, Y., Harell, A., and Somjen, D. Hormonal stimulation of bone cell proliferation. J Steroid Biochem Mol Biol 37:431-435; 1990.
19. Keeting, P. E., Scott, R. E., Colvard, D. S., Han, I. K., Spelsberg, T. C., and Riggs, B. L. Lack of a direct effect of estrogen on proliferation and differentiation of normal human osteoblast-like cells. J Bone Miner Res 6:297-304; 1991.
20. Komm, B. S., Terpening, C. M., Benz, D. J., Graeme, K. A., Gallegos, A., Korc, M., Greene, G. L., O'Malley, B. W., and Haussler, M. R. Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science 241:81-84; 1988.
21. Lajeunesse, D. Effect of 17β-estradiol on the human osteosarcoma cell line MG63. Bone Miner 24:1-16; 1994.
22. Lamberg-Allardt, C. Is there a role for vitamin D in osteoporosis? Calcif Tissue Int 49(Suppl.):S46-S49; 1991.
23. Liel, Y., Kraus, S., Levy, J., and Shany, S. Evidence that estrogens modulate activity and increase the number of 1,25-dihydroxyvitamin D receptors in osteoblast-like cells (ROS 17/2.8). Endocrinology 130:2597-2601; 1992.
24. Lowry, O. H. Micromethods for the assay of enzyme. II. Specific procedures: Alkaline phosphatase. Methods Enzymol 4:371-372; 1955.
25. Migliaccio, S., Davis, V. L., Gibson, M. K., Gray, T. K., and Korach, K. S. Estrogens modulate the responsiveness of osteoblast-like cells (ROS 17/2.8) stably transfected with estrogen receptor. Endocrinology 130:2617-2624; 1992.
26. Murray, E., Provvedini, D., Curran, D., Catherwood, B., Sussman, H., and Manolagas, S. Characterization of a human osteoblast osteosarcoma cell line (SaOS-2) with high bone alkaline phosphatase. J Bone Miner Res 2:231-238; 1987.
27. Oursler, M. J., Landers, J. P., Riggs, B. L., and Spelsberg, T. C. Oestrogen effects on osteoblasts and osteoclasts. Ann Med 25:361-371; 1993.
28. 3 and its analogues are differentiation-stage dependent. Bone 16(Suppl. 1):218S; 1995.
29. Rao, L. G., Wylie, J. N., Kung Sutherland, M. S., and Murray, T. M. I7β-estradiol and parathyroid hormone potentiate each other's stimulatory effects on alkaline phosphatase activity in SaOS-2 cells in a differentiation dependent manner. Endocrinology 134:614-620; 1994.
30. 3 on alkaline phosphatase activity in human osteosarcoma cells in a differentiation-dependent manner. J Endocrinol 148:181-187; 1996.
31. Rickard, D. J., Gowen, M., and MacDonald, B. R. Proliferative responses to estradiol, IL-1 alpha and TGF-beta by cells expressing alkaline phosphatase in human osteoblast-like cell cultures. Calcif Tissue Int 52:227-233; 1993.
32. Roemer, K. and Friedemann, T. Modulation of cell proliferation and gene expression by a p53-estrogen receptor hybrid protein. Proc Natl Acad Sci USA 90:9252-9256; 1993.
33. Scheven, B. A. A., Damen, C. A., Hamilton, N. J., Verhan, H. J. J., and Duursma, S. A. Stimulatory effects of estrogen and progesterone on proliferation and differentiation of normal human osteoblast-like cells in vitro. Biochem Biophys Res Commun 186:54-60; 1992.
34. 3 on alkaline phosphatase in SaOS-2 cells is associated with enhanced estrogen receptor levels. J Bone Miner Res 9(Suppl. 1):S170; 1994.
35. Toulas, C., Beauregard, G., Delassus, F., Thauvette, L., Potier, M., Bayard, F., and Faye, I.-Ch. Target size analysis of estrogen receptor in cultured intact cells: change in receptor structure between subconfluency and superconfluency in culture. Mol Cell Endocrinol 72:89-94; 1990.
36. Watts, C. K. W., Parker, M. G., and King, R. J. B. Stable transfection of the oestrogen receptor gene into a human osteosarcoma cell line. J Steroid Biochem 34:483-490; 1989.
37. Wong, M. M., Rao, I. G., Ly, H., Hamilton, L., Tong, J., Sturtridge, W., McBroom, R., Aubin, J. E., and Murray, T. M. Long-term effects of physiological concentrations of dexamethasone on human bone-derived cells. J Bone Miner Res 5:803-813; 1990.
38. Wong, S. Y., Purdie, A., Sewell, H. F., Wilkinson, L., Angus, B., Westley, B., and Home, C. H. W. Immunohistochemical assessment of ER-P31, a mouse anti-oestrogen receptor protein monoclonal antibody in human breast cancers: Comparison with ER-ICA (Abbott) and radioligand assays. Tumor Biol 12:16-23; 1991.