Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene

Journal of Virological Methods

Volumn 60, Issue 1, 1996, Pages 29-37

  Zhang, Zhiying ^a   Martineau, Daniel ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

'Touchdown' PCR; Fish; Nested PCR; Nucleic acid; Retrovirus; Sarcoma; Tumor

Indexed keywords

VIRUS ENVELOPE PROTEIN;

ARTICLE; DNA SEQUENCE; FISH; GENETIC VARIABILITY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RETROVIRUS; SARCOMA VIRUS; VIRUS DETECTION;

EID: 0030005584     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(96)02024-1     Document Type: Article

References (7)

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3. Don, R.H., Cox, P.T., Wainright, B.J., Baker, K. and Mattick, J.S. (1991) 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucl. Acids Res. 19(14), 4008.
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7. Ulrich, P.P., Romeo, J.M., Daniel, L.J. and Vyas, G.N. (1995) An improved method for the detection of hepatitis C virus RNA in plasma utilizing heminested primers and internal control RNA. PCR Methods Applic 2, 241-249.