A CD66a-specific, activation-dependent epitope detected by recombinant human single chain fragments (scFvs) on CHO transfectants and activated granulocytes

Journal of Leukocyte Biology

Volumn 59, Issue 6, 1996, Pages 891-901

  Jantscheff, Peter ^a,d   Nagel, Gerhard ^a   Thompson, John ^a   Kleist, Sabine V ^a   Embleton, M J ^b,c,e   Price, Michael R ^b,c   Grunert, Fritz ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^d UNIVERSITY HOSPITAL BASEL   (Switzerland)

^e CHRISTIE HOSPITAL   (United Kingdom)

Author keywords

Biliary glycoproteins; CEA family; HL60; Neuraminidase; Phorbol myristate acetate; Retinoic acid

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; EPITOPE; FORMYLMETHIONYLLEUCYLPHENYLALANINE; IMMUNOGLOBULIN FRAGMENT; PHORBOL 13 ACETATE 12 MYRISTATE; RECOMBINANT PROTEIN; RETINOIC ACID; SIALIDASE;

ANIMAL CELL; ANTIGEN EXPRESSION; ANTIGEN SPECIFICITY; ARTICLE; CELL STRAIN HL 60; CHO CELL; GENETIC TRANSFECTION; GRANULOCYTE; HUMAN; HUMAN CELL; LEUKOCYTE ACTIVATION; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0030005463     PISSN: 07415400     EISSN: None     Source Type: Journal    
DOI: 10.1002/jlb.59.6.891     Document Type: Article

References (63)

1. Thompson, J. A., Grunert, F., Zimmermann, W. (1991) Carcinoembryonic antigen gene family: Molecular biology and clinical perspectives. J. Clin. Lab. Anal. 5, 344-366.
2. Nagel, G., Grunert, F., Kuijpers, T. W., Watt, S. M., Thompson, J., Zimmermann, W. (1993) Genomic organization, splice variants and expression of CGM1, a CD66-related member of the carcinoembryonic antigen gene family. Eur. J. Biochem. 214, 27-35.
3. Van der Schoot, C. E., Huizinga, T. W. J., Gadd, S., Majdic, O., Wijmans, R., Knapp, W., Von dem Borne, A. E. G. Kr. (1989) Identification of three novel PI-linked proteins on granulocytes. In Leukocyte Typing IV, (W. Knapp, B. Dörken, W. R. Gilks, E. D. Rieber, R. E. Schmidt, H. Stein, and A. E. G. Kr. von dem Borne, eds.), New York, Oxford University Press, 887-891.
4. Watt, S. M., Sala-Newby, G., Hoang, T., Gilmore, D. J., Grunert, F., Nagel, G., Murdoch, S. J., Tchilian, E., Lennox, E. S., Waldmann, H. (1991) CD 66 identifies a neutrophil-specific epitope within the haemopoietic system that is expressed by members of the CEA-family of adhesion molecules. Blood 78, 63-74.
5. Skubitz, K., Micklem, K., van der Schoot, E. (1995) CD66 and CD67 cluster workshop report. In Leukocyte Typing V, White Cell Differentiation Antigens (S. F. Schlossmann, L. Boumsell, W. Gilks, J. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, T. A. Springer, T. F. Tedder, and R. F. Todd, eds.), New York, Oxford University Press, 889-899.
6. Grunert, F., Soubt, M., Jantscheff, P., Nagel, G. (1994) Specificity and epitope localization of CD66/CD67 mAb. In Leukocyte Typing V. White Cell Differentiation Antigens (S. F. Schlossman, L. Boumsell, W. Gilks, J. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, S. Shaw, T. A. Springer, T. F. Tedder, and R. F. Todd, eds.), New York, Oxford University Press, 907-911.
7. Grunert, F., Nagel, G., Jantscheff, P. (1993) Specificity and epitope-localization of CD66/67 mAb tested by transfectants expressing members of the CEA-family. Tissue Antigen 42, 89 (abstract).
8. Stoffel, A., Neumaier, M., Gaida, F. J., Fenger, U., Drzeniek, Z., Hauberk. H. D., Wagener, C. (1993) Monoclonal, anti-domain and anti-peptide antibodies assign the molecular weight 160,000 granulocyte membrane antigen of the CD66 cluster to a mRNA species encoded by the biliary glycoprotein gene, a member of the carcinoembryonic antigen gene family. J. Immunol 150, 4978-4984.
9. ++-dependent intercellular adhesion molecule. Cell Growth Diff. 1, 527-533.
10. Watt, S. M., Fawcett, J., Murdoch, S. J., Teixeira, A. M., Gschmeissner, S. E., Hajibagheri, N. M. A. N., Simmons, D. L. (1994) CD66 identifies the biliary glycoprotein (BGP) adhesion molecule: cloning, expression, and adhesion functions of the BGPc splice variant. Blood 84, 200-210.
11. ++-independent cell adhesion activities of biliary glycoprotein, a member of carcinoembryonic antigen family, expressed on CHO cell surface. Biochem. Biophys. Res. Commun. 186, 881-887.
12. Oikawa, S., Inuzuka, C., Kuroki, M., Arakawa, F., Matsuoka, Y., Kosaki, G., Nakazato, H. (1991) A specific heterotypic cell adhesion activity between members of carcinoembryonic antigen family, W272 and NCA, is mediated by N-domains. J. Biol. Chem. 266, 7995-8001.
13. Stocks, S. C., Albrechtsen, M., Kerr, M. A. (1990) Expression of the CD15 differentiation antigen (3-fucosyl-N-acetyl-lactosamin, LeX) on putative neutrophil adhesion molecules CR3 and NCA-160. Biochem. J. 268, 275-280.
14. Kuijpers, T. W., Hoogerwerf, M., van der Laan, L. J. W., Nagel, G., van der Schoot, C., Grunert, F., Roos, D. (1992) CD66 nonspecific cross-reacting antigens are involved in neutrophil adherence to cytokine-activated endothelial cells. J. Cell Biol. 118, 457-466.
15. Benchimol, S., Fuks, A., Jothy, S., Beauchemin, N., Shirota, K., Stanners, C. P. (1989) Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule. Cell 57, 327-334.
16. Barnett, T. R., Drake, L., Pickle II, W. (1993) Human biliary glycoprotein gene: Characterization of a family of novel alternatively spliced RNAs and their expressed proteins. Mol. Cell Biol. 13, 1273-1282.
17. Barnett, T. R., Kretschmer, A., Austen, D. A., Goebel, S. J., Hart, J. T., Elting, J. J., Kamarck, M. E. (1989) Carcinoembryonic antigens: Alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family. J. Cell Biol, 108, 267-276.
18. Lund-Johansen, F., Olweus, J., Symington, F. W., Arli, A., Thompson, J. S., Vilella, R., Skubitz, K., Horejsi, V. (1993) Activation of human monocytes and granulocytes by monoclonal antibodies to glycosylphosphatidylinositol-anchored antigens. Eur. J. Immunol. 23, 2782-2791.
19. Skubitz, K. M., Ducker, T. P., Skubitz, A. P. N., Goueli, S. A. (1993) Antiserum to carcinoembryonic antigen recognizes a phosphotyrosine-containing protein in human colon cancer cell lines. FEBS Lett. 318, 200-204.
20. Neumaier, M., Paululat, S., Chan, A., Matthaes, P., Wagener, C. (1993) Biliary glycoprotein, a potential human cell adhesion molecule, is down-regulated in colorectal carcinomas. Proc. Natl. Acad. Sci. USA 90, 10744-10748.
21. Thompson, J., Zimmermann, W., Nollaus, P., Neumaier, M., Weber-Arden, J., Schrewe, H., Craig, I., Willcocks, (1994) CGM2, a member of the carcinoembryonic antigen gene family is down-regulated in colorectal carcinomas. J. Biol. Chem. 269, 32924-32931.
22. Hsieh, J. T., Luo, W. P., Song, W. T., Wang, Y., Kleinerman, D. I., Van, N. T., Lin, S. H. (1995) Tumor suppressive role of an androgen-regulated epithelial cell adhesion molecule (C-CAM) in prostate carcinoma cell revealed by sense and antisense approaches. Cancer Res. 55, 190-197.
23. Kleinerman, D. I., Troncoso, P., Lin, S. H., Pisters, L. L., Sherwood, E. R., Brooks, T., Voneschenbach, A. C., Hsieh, J. T. (1995) Consistent expression of an epithelial cell adhesion molecule (C-CAM) during human prostate developmant and loss of expression in prostate cancer - implication as a tumor suppressor. Cancer Res. 55, 1215-1220.
24. Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths, A. D., Winter, G. (1991) By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
25. Griffiths, A. D., Malmqvist, M., Marks, J. D., Bye, J. M., Embleton, M. J., McCafferty, J., Baier, M., Hollinger, K. P., Gorick, B. D., Hughes-Jones, N. C., Hoogenboom, H. R., Winter, C. (1993) Human anti-self antibodies with high specificity from phage display libraries. EMBO J. 12, 725-734.
26. Jantscheff, P., Böttger, V., Price, M., Micheel, B., Kaiser, G., Zotter, S., Kotzsch, M., Großmann, H., Karaten, U. (1991) Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA). Biomed. Biochim. Acta 50, 1261-1267.
27. Daniel, S., Nagel, G., Johnson, J. P., Lobo, F. M., Hirn, M., Jantscheff, P., Kuroki, M., von Kleist, S., Grunert, F. (1993) Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants. Int. J. Cancer 55, 303-310.
28. Pèlegrin, A., Terskikh, A., Hayoz, D., Chalandon, Y., Olsson, N.-O., Folli, S., Buchegger, F., Kromer, B., Schwarz, K., Martin, M., Martin, F., Mach, J.-P. (1992) Human carcinoembryonic antigen cDNA expressed in rat carcinoma cells can function as a target antigen for tumor localization of antibodies in nude rats and as rejection antigen in syngeneic rats. Int. J. Cancer 52, 110-119.
29. Berling, B., Kolbinger, F., Grunert, F., Thompson, J. A., Brombacher, F., Buchegger, F., von Kleist, S., Zimmermann, W. (1990) Cloning of a carcinoembryonic antigen family member expressed in leukocytes of chronic myeloid leukaemia patients and bone marrow. Cancer Res. 50, 6334-6539.
30. Kuroki, M., Arakawa, F., Matsuo, Y., Oikawa, S., Misumi, Y., Nakazato, H., Matsuoka, Y. (1991) Molecular cloning of nonspecific cross-reacting antigens in human granulocytes. J. Biol. Chem. 206, 11810-11817.
31. Jantscheff, P., Winkler, L., Karawajew, L., Kaiser, G., Böttger, V., Micheel, B. (1993) Hybrid hybridomas producing bispecific antibodies to CEA and peroxidase isolated by a combination of HAT medium selection and fluorescence activated cell sorting. J Immunol. Meth. 163, 91-97.
32. Novak-Hofer, I., Bläuenstein, P., Schubiger, P. A. (1990) Regulation of the cell surface expression of a nonspecific cross-reacting antigen variant during differentiation of HL-60 cells. Cancer Res. 50, 7437-7443.
33. Stöckbauer, P., Gahmberg, C. G., Andersson, L. C. (1985) Changes in cell surface glycoproteins and antigens during differentiation of the human myeloid cell lines ML-1, ML-2, and HL-60. Cancer Res. 45, 2821-2826.
34. Breitman, T. R., Selonick, S. E., Collins, S. J. (1980) Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc. Natl. Acad. Sci. USA 77, 2936-2941.
35. Bass, D. A., Parce, J. W., DeChatelet, L. R., Szejda, P., Seeds, M. C., Thomas, M. (1983) Flow cytometric studies of oxidative product formation by neutrophils: a graded response Io membrane stimulation. J. Immunol. 130, 1910.
36. Hammarström, S., Shively, J. E., Paxton, R. J., Beatty, B. G., Larsson, A., Ghosh, R., Börmer, O., Buchegger, F., Mach, J.-P., Burtin, P., Seguin, P., Darbouret, B., Degorce, F., Sertour, J., Jolu, J. P., Fuks, A., Kalthoff, H., Schmiegel, W., Arndt, R., Klöppel, G., von Kleist, S., Grunert, F., Schwarz, K., Matsuoka, Y., Kuroki, M. (1989) Antigenic sites in carcinoembryonic antigen. Cancer Res. 49, 4852-4858.
37. Kawahara, M., Chia, D., Terasaki, P. I., Roumanas, A., Sugich, L., Hermes, L., Iguro, T. (1985) Detection of sialylated lewis x antigen in cancer sera using a sandwich radioimmunoassay. Int. J. Cancer 36, 421-425.
38. Dubois, C., Schlageter, M. H., de Gentile, A., Balitrand, N., Toubert, M. E., Krawice, I., Fenaux, P., Castaigne, S., Najean, Y., Degos, L. (1994) Modulation of IL-8, IL-1 beta, and C-CSF secretion by all-trans retinoic acid in acute promyelocytic leukemia. Leukemia 8, 1750-1757.
39. Burn, T. C., Petrovick, M. S., Hohaus, S., Rollins, B. J., Tenen, D. G. (1994) Monocyte chemoattractant protein-1 gene is expressed in activated neutrophils and retinoic acid-induced human myeloid cell lines. Blood 84, 2776-2783.
40. Diamond, M. S., Springer, T. A. (1993) A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J. Cell Biol. 120, 545-556.
41. Arroyo, A. G., Garcia-Vicuna, R., Marazuela, M., Yednock, T. A., Gonzalez-Amaro, R., Sanchez-Madrid, F. (1995) Expression and functional significance of an activation-dependent epitope of the beta 1 integrins in chronic inflammatory diseases. Eur. J. Immunol. 25, 1720-1728.
42. Lenter, M., Uhlig, H., Hamann, A., Jeno, P., Imhof, B., Vestweber, D. (1993) A monoclonal antibody against an activation epitope on mouse integrin chain beta 1 blocks adhesion of lymphocytes to the endothelial integrin alpha 6 beta 1. Proc. Natl. Acad. Sci. USA 90, 9051-9055.
43. Afar, D. E. H., Stanners, C. P., Bell, J. C. (1992) Tyrosine phosphorylation of biliary glycoprotein, a cell adhesion molecule related to carcinoembryonic antigen. Biochim. Biophys. Acta 1134, 46-52.
44. Skubitz, K. M., Ducker, T. P., Goueli, S. A. (1992) CD66 monoclonal antibodies recognize a phosphotyrosine-containing protein bearing a carcinoembryonic antigen crossreacting antigen on the surface of human neutrophils. J. Immunol. 148, 852-860.
45. Teixeira, A. M., Fawcett, J., Simmons, D. L., Watt, S. M. (1994) The N-domain of the biliary glycoprotein (BGP) adhesion molecule mediates homotypic binding: Domain interactions and epitope analysis of BGPc. Blood 84, 211-219.
46. Kobata, A., Totani, K., Endo, T., Kuroki, M., Matsuoka, Y., Yamashita, K. (1989) Comparative study of the sugar chains of carcinoembryonic antigen and its crossreacting antigen purified from meconium. In The Carcinoembryonic Antigen Gene Family (A. Yachi and J. E. Shively eds.), Amsterdam, Elsevier, 12-22.
47. Nagata, A., Miura, M., Komoda, T. (1991) Sandwich assay for carcinoembryonic antigen with immobilized lectins and a monoclonal antibody. Tumor Biol. 12, 35-44.
48. Hernando, J. J., von Kleist, S., Grunert, F. (1994) A repertoire of monoclonal antibodies reveals extensive epitope heterogeneity in CEA purified from neoplasms originating from different organs. Int. J. Cancer 56, 655-661.
49. Hefta, S. A., Paxton, R. J., Shively, J. E. (1990) Sequence and glycosylation site identity of two distinct glycoforms of nonspecific cross-reacting antigen as demonstrated by sequence analysis and fast atom bombardment mass spectrometry. J. Biol. Chem. 265, 8618-8626.
50. Mackiewicz, A., Schultz, D., Mathison, J., Ganapathi, M., Kushner, I. (1989) Effect of cytokines on glycosylation of acute phase proteins in hepatoma cell lines. Clin. Exp. Immunol. 75, 70-75.
51. Cross, A. S., Wright, D. G. (1991) Mobilization of sialidase from intracellular stores to the surface of human neutrophils and its role in stimulated adhesion responses of these cells. J. Clin. Invest. 88, 2067-2076.
52. Ducker, T. P., Skubitz, K. M. (1992) Subcelluar localization of CD66, CD67, and NCA in human neutrophils. J. Leukoc. Biol. 52, 11-16.
53. Kuroki, M., Matsuo, Y., Kinugasa, T., Matsuoka, Y. (1992) Augmented expression and release of nonspecific cross-reacting antigens (NCAs), members of the CEA family, by human neutrophils during cell activation. J. Leukoc. Biol. 52, 551-557.
54. Bazil, V. (1995) Physiological enzymatic cleavage of leukocyte membrane molecules. Immunol. Today 16, 135-140.
55. Borregaard, N., Lollike, K., Kjeldsen, L., Sengelov, H., Bastholm, L., Nielsen, M. H., Bainton, D. F. (1993) Human neutrophil granules and secretory vesicles. Eur. J. Haematol. 51, 187-198.
56. Hallett, M. B., Lloyds, D. (1995) Neutrophil priming: the cellular signals that say 'amber' but not 'green'. Immunol. Today 16, 264-268.
57. Blechman, J. M., Lev, S., Barg, J., Eisenstein, M., Vaks, B., Vogel, Z., Givol, D., Yarden, Y. (1995) The fourth immunoglobulin domain of the stem cell receptor couples ligand binding to signal transduction. Cell 80, 103-113.
58. Van Seventer, G. A., Shimizu, Y., Horgan, J., Luce, G. E. G., Webb, D., Shaw, S. (1991) Remote T cell co-stimulation via LFA-1/ICAM-1 and CD2/LFA-3: demonstration with immobilized ligand/mAb and implication in monocyte co-stimulation. Eur. J. Immunol. 21, 1711.
59. Rothlein, R., Kishimoto, T. K., Mainolfi, E. (1994) Cross-linking of ICAM-1 induces co-signaling of an oxidative burst from mononuclear leukocytes. J. Immunol. 152, 2488-2495.
60. Reth, M. (1989) Antigen receptor tail clue. Nature 338, 383-384.
61. Keegan, A. D., Paul, W. E. (1992) Multichain immune recognition receptors: similarities in structure and signaling pathways. Immunol. Today 13, 63-68.
62. Joseph, K., Hofften, H., Bosslet, K., Schorlemmer, H. U. (1988) In vivo labelling of granulocytes with 99mTC anti-NCA monoclonal antibodies for immaging inflammation. Eur. J. Nucl. Med. 14, 367-373.
63. Reske, S. N., Karstens, J. H., Gloeckner, W., Steinstrasser, A., Schwarz, A., Ammon, J., Buell, U. (1989) Radioimmunoimaging for diagnosis of bone marrow involement in breast cancer and malignant lymphoma. Lancet 1, 299-301.