Comparison between internal microviscosity of low-density erythrocytes and the microviscosity of hemoglobin solutions: An electron paramagnetic resonance study

Biophysical Journal

Volumn 71, Issue 1, 1996, Pages 389-393

  Gennaro, A M ^a,c   Luquita, A ^b   Rasia, M ^b  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

^b FACULTAD DE CIENCIAS MÉDICAS   (Argentina)

^c Instituto de Desarrollo Tecnologico para la Industria Quimica   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE; HEMOGLOBIN;

ARTICLE; BLOOD RHEOLOGY; CONTROLLED STUDY; CYTOPLASM; DENSITY GRADIENT; ELECTRON SPIN RESONANCE; ERYTHROCYTE; ERYTHROCYTE SHAPE; ERYTHROCYTE VOLUME; HUMAN; HUMAN CELL; MEAN CORPUSCULAR HEMOGLOBIN; SPIN LABELING; VISCOSITY;

EID: 0030004339     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79239-1     Document Type: Article

References (24)

1. Bales, B. 1989. Experimental methods in spin-label spectral analysis. In Biological Magnetic Resonance, Vol. 8, Spin Labeling - Theory and Applications. L. J. Berliner and J. Reuben, editors. Plenum Press, New York. 77-130.
2. Beaudoin, A. G., and H. Mizukami. 1978. ESR correlation times of 2,2,6,6-tetramethyl piperidone-N-oxyl (Tempone) in solutions of hemoglobin A and hemoglobin S. Biochim. Biophys. Acta. 532:41-47.
3. Cokelet, G. R., and H. J. Meiselman. 1968. Rheological comparison of hemoglobin solutions and erythrocyte suspensions. Science. 162: 275-277.
4. Daveloose, D., G. Fabre, F. Berleur, G. Testylier, and F. Leterrier. 1983. A new spin-label method for the measurement of erythrocyte internal microviscosity. Biochim. Biophys. Acta. 763:41-49.
5. Dintenfass, L. 1971. Blood Microrheology. Viscosity Factors in Blood Flow, Ischaemia and Thrombosis. Appleton-Century-Crofts, New York. 445 pp.
6. Endre, Z. H., and P. W. Kuchel. 1986. Viscosity of concentrated solutions and of human erythrocyte cytoplasm determined from NMR measurement of molecular correlation times. The dependence of viscosity on cell volume. Biophys. Chem. 24:337-356.
7. Eriksson, L. E., and H. Beving. 1993. Calcium and lead-activated morphological changes in human erythrocytes: a spin-label study of the cytoplasm. Arch. Biochem. Biophys. 303:296-301.
8. Herrmann, A., and P. Muller. 1986. Correlation of the internal microviscosity of human erythrocytes to the cell volume and the viscosity of hemoglobin solutions. Biochim. Biophys. Acta. 885:80-87.
9. Kivelson, D. 1972. Electron spin relaxation in liquids. Selected topics. In Electron Spin Relaxation in Liquids. L. T. Muus and P. W. Atkins, editors. Plenum Press, New York. 213-277.
10. Luquita, A., A. M. Gennaro, and M. Rasia. 1996. Effects of subnormal hemoglobin concentration on the deformability of normocytic erythrocytes. Clin. Hemorheol. 16:117-127.
11. Mach, O., and L. Lacko. 1968. Density gradient in a dextran medium. Anal. Biochem. 22:393-397.
12. Morse, P. D., II, D. M. Lusczakoski, and D. A. Simpson. 1979. Internal microviscosity of red blood cells and hemoglobin-free resealed ghosts: a spin-label study. Biochemistry. 18:5021-5029.
13. Morse, P. D., II, M. Ruhlig, W. Snipes, and A. D. Keith. 1975. A spin-label study of the viscosity profile of sarcoplasmic reticular vesicles. Arch. Biochem. Biophys. 168:40-56.
14. Morse, P. D., II, and J. A. Warth. 1990. Direct measurement of the internal microviscosity of sickle erythrocytes as a function of cell density. Biochim. Biophys. Acta. 1053:49-55.
15. Nash, G. B., and H. J. Meiselman. 1983. Red cell and ghost viscoelasticity. Effects of hemoglobin concentration and in vivo aging. Biophys. J. 43:63-73.
16. Raison, J. K., J. M. Lyons, R. J. Mehlborn, and A. D. Keith. 1971. Temperature-induced phase changes in mitochondrial membranes detected by spin labeling. J. Biol. Chem. 246:4036-4040.
17. Ross, P. D., and A. P. Minton. 1977. Hard quasispherical model for the viscosity of hemoglobin solutions. Biochem. Biophys. Res. Commun. 76:971-976.
18. Schwoch, G., and H. Passow. 1973. Preparation and properties of human erythrocyte ghosts. Mol. Cell. Biochem. 2:197-218.
19. Sutera, S. P., R. A. Gardner, C. W. Boylan, G. L. Carroll, K. C. Chang, J. S. Marvel, C. Kilo, B. Gonen, and J. R. Williamson. 1985. Age-related changes in deformability of human erythrocytes. Blood. 65:275-282.
20. Tanford, C. 1961. Physical Chemistry of Macromolecules. John Wiley & Sons, New York. 710 pp.
21. Tanford, C. 1980. The Hydrophobic Effect: Formation of Micelles and Biological Membranes, 2nd ed. John Wiley & Sons, New York. 233 pp.
22. Voet, D., and J. G. Voet. 1990. Biochemistry. John Wiley & Sons, New York. 1223 pp.
23. Wertz, J. E., and J. R. Bolton. 1972. Electron Spin Resonance. Elemental Theory and Practical Applications. McGraw-Hill, Inc., New York. 497 pp.
24. Wintrobe, M. M. 1981. Clinical Hematology, 8th ed. Lea & Febiger, Philadelphia, PA. 2021 pp.