Reducing "double sequences" in automated DNA sequencing with T7 DNA polymerase and internal labeling

BioTechniques

Volumn 20, Issue 5, 1996, Pages 791-792

  Wiemann, S ^a   Schilke, A ^a   Rechmann, S ^a   Zimmermann, J ^a   Voss, H ^a   Ansorge, W ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE;

AUTOMATION; BACTERIOPHAGE T7; DNA SEQUENCE; HUMAN; LABORATORY TEST; SEQUENCE ANALYSIS; SHORT SURVEY;

AUTOMATION; BASE SEQUENCE; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA;

EID: 0030003179     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205bm12     Document Type: Article

References (4)

1. Studier, F.W. 1989. A strategy for high-volume sequencing of cosmid DNAs: random and direct priming with a library of oligonucleotides. Proc. Natl. Acad. Sci. USA 86:6917-6921.
2. Tabor, S. and C.C. Richardson. 1989. Selective inactivation of the exonuclease activity of bacteriophage T7 DNA polymerase by in vitro mutagenesis. J. Biol. Chem. 264:6447-6458.
3. Voss, H., S. Wiemann, U. Wirkner, C. Schwager, J. Zimmermann, J. Stegemann, H. Erfle, N. Hewitt, T. Rupp and W. Ansorge. 1992. Automated DNA sequencing system resolving 1000 bases with fluorescein-15-dATP as internal label. Meth. Mol. Cell. Biol. 3:153-155.
4. Wiemann, S., T. Rupp, J. Zimmermann, H. Voss, C. Schwager and W. Ansorge. 1995. Primer design for automated DNA sequencing utilizing T7 DNA polymerase and internal labeling with fluorescein-15-dATP. BioTechniques 18:688-697.