Comparison of microscope techniques for the examination of biofilms

Journal of Microbiological Methods

Volumn 25, Issue 1, 1996, Pages 57-70

  Surman, S B ^a   Walker, J T ^b   Goddard, D T ^c   Morton, L H G ^d   Keevil, C W ^b   Weaver, W ^c   Skinner, A ^e   Hanson, K ^f   Caldwell, D ^f   Kurtz, J ^e  

^a QUEEN S MEDICAL CENTRE   (United Kingdom)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

^c SELLAFIELD LTD   (United Kingdom)

^d UNIVERSITY OF CENTRAL LANCASHIRE   (United Kingdom)

^e JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^f UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

atomic force microscopy; biofilms; electron microscopy; fluorescence microscopy; light microscopy; scanning confocal laser microscopy

Indexed keywords

ARTICLE; ATOMIC FORCE MICROSCOPY; BIOFILM; CONTROLLED STUDY; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; LASER MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; ULTRASTRUCTURE;

EID: 0030000173     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-7012(95)00085-2     Document Type: Article

References (24)

1. Lawrence, J.R., Korber, D.R., Hoyle, B.D., Costerton, J.W., Caldwell, D.J. (1991) Optical sectioning of microbial biofilms. J. Bacteriol. 53, 2714-2724.
2. Denyer, S.P. and Lynn, R. (1987) A sensitive method for the rapid detection of bacterial contaminants in intravenous fluids. J. Parent. Sci. Technol. 41, 60-66
3. Bakke, R. and Olsson, P. (1986) Biofilm thickness measurements by light microscopy. J. Microbiol. Methods 5, 93-98.
4. Rogers, J. and Keevil, C.W. (1992) Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualised by using episcopic differential interference contrast microscopy. Appl. Environ. Microbiol. 58, 7, 2326-2330.
5. Rodriguez, G., Phipps, G.D., Ishiguro, K. and Ridgway, H.F. (1992) Use of a fluorescent redox probe for direct visualisation of actively respiring bacteria. Appl. Environ. Microbiol. 58, 6, 1801-1808.
6. Little, B., Wagner, P., Ray, R., Pope, R. and Scheetz, R. (1991) Biofilms: an ESEM evaluation of artefacts introduced during SEM preparation. J. Indust. Microbiol. 8, 213-222.
7. Goddard, D.T., Weaver, W., Steele, A., and Harron, H. (1993) Imaging soft and delicate materials. Mat. World 1, 616-617.
8. Caldwell, D.E., Korber, D.R., Lawrence, J.R. (1992) Confocal laser microscopy and computer image analysis. In: Marshall, K.C. (ed) Advances Microbial Ecology. Plenum Press, New York. 12, pp 1-6
9. Cummins, D., Moss, M.C., Jones, C, L., Howard, C.V. and Cummins, P.G. (1992) Confocal Microscopy on dental plaque development. Binary 4, 86-91.
10. Keevil, C.W., Bradshaw, D.J., Dowsett, A.B. and Feary, T.W. (1987) Microbial film formation: dental plaque deposition on acrylic tiles using continuous culture, J. Appl. Bacteriol. 62, 129-138.
11. Keevil, C.W., Glenister, D.A., Salamon, K.E., Dennis, P.J. and West, A.A. (1988) A continuous culture biofilm model for the study of medical and industrial corrosion, In: Proceedings of the Spring meeting Biodetioration. Society, 4: Biofilms, (Morton, L.H. G. and Chamberlain, A.H.L., eds.) pp 48-62.
12. Colbourne, J.C., Trew, R.M. and Dennis, P.J. (1988) Treatment of water for aquatic bacterial growth studies. J. Appl. Bacteriol. 65, 79-85.
13. Hoffman, R. (1988) Applications of the Modulation Contrast microscope, International Laboratory, July/ August, 32-39.
14. Keevil, C.W. and Walker, J.T. (1992) Normaski DIC microscopy and image analysis of biofilms. Binary 4, 93-95.
15. Caldwell, D.E., Korber, D.R. and J.R. Lawrence. (1992) Imaging of bacterial cells by fluorecence exclusion using scanning confocal laser microscopy. J. Microbiol. Methods. 15, 249-261,
16. Rodriquez, G., Phipps, G., Ishiguro, D. and Ridgway, H. F. (1992) Use of a fluroescent redox probe for direct visualisation of actively respiring bactera. Appl. Environmen. Microbiol. 58, 1801-1908.
17. Stewart, P.S., Griebe, T., Srinvasan, Chen, C.I. Yu, I.P., deBeer, D and McFeters, G.A. (1994) Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms. App. Environ. Microbiol. 31, 1690-1692.
18. Angell, P. and Chamberlain, A.H.L. (1991) The role of extracellular products in copper colonisation. Int. Biodet. 27, 135-143.
19. Fischer, W., Hanßel, I. and Paradies, H.H. (1988) First results of microbial induced corrosion of copper pipes. In: Microbial Corrosion. (Eds. C.A.C. Sequira and A.K. Tiller) Elsevier Applied Science. London. 7, 300-327.
20. Lambe, D.W., Ferguson, K.P. and Ferguson, D.A. (1988) The Bacteroides glycocalyx as visualised by differential interference contrast microscopy. Can. J. Microbiol. 34, 1195.1189-
21. Costerton, J.W., Nickel, J.G. and Ladd, T.I. (1986) Suitable methods for the comparative study of free living and surface associated bacterial populations. In: Bacteria in Nature Vol. 2. (Poindexter, J.S. and Leadbetter, E.R., eds.), Plenum Press. New York.
22. Costerton, J.W. (1979) The role of the electron microscope in the elucidation of bacterial structure and function, Ann. Rev. Microbiol. 33, 459-79.
23. Sutton, N.A., Hughes, N. and Handley, P. (1994) A comparison of conventional SEM techniques, low temperature SEM and the electroscan wet scanning electron microscope to study the structure of a biofilm of Streptococcus crista CR3. J. App. Bacteriol. 76, 448-454.
24. Slayter, E.M. and Slayter, H.S. (1992) Light and Electron Microscopy. Cambridge University Press.