Ste50p sustains mating pheromone-induced signal transduction in the yeast Saccharomyces cerevisiae

Molecular Microbiology

Volumn 20, Issue 4, 1996, Pages 773-783

  Xu, Gang ^a   Jansen, Gregor ^a   Thomas, David Y ^b   Hollenberg, Cornelis P ^a   Rad, Massoud Ramezani ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PHEROMONE;

ARTICLE; CONTROLLED STUDY; GENETIC ANALYSIS; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

ALLELES; FUNGAL PROTEINS; GTP-BINDING PROTEINS; MAP KINASE KINASE KINASES; PEPTIDES; PHEROMONES; PROTEIN-SERINE-THREONINE KINASES; RECEPTORS, MATING FACTOR; RECEPTORS, PEPTIDE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES POMBE PROTEINS; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS;

EID: 0029999863     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02516.x     Document Type: Article

References (62)

1. Alani, E., Cao, L., and Kleckner, N. (1987) A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545.
2. Blinder, D., Bouvier, S., and Jenness, D.D. (1989) Constitutive mutants in the yeast pheromone response: ordered function of the gene products. Cell 56: 479-486.
3. Blumer, K.J., and Thorner, J. (1990) β and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the α subunit but are required for receptor coupling. Proc Natl Acad Sci USA 87: 4363-4367.
4. Bonneaud, N., Ozier-Kalogeropoulos, O., Li, G., Labouesse, M., Minvielle-Sebastia, L., and Lacroute, F. (1991) A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615.
5. Burkholder, A.C., and Hartwell, L.H. (1985) The yeast α-factor receptor: structural properties deduced from the sequence of the STE2 gene. Nucl Acids Res 13: 8463-8475.
6. Byers, B., and Goetsch, L. (1975) Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae. J Bacteriol 124: 511-523.
7. Cairns, B.R., Ramer, S.W., and Kornberg, R.D. (1992) Order of action of components in the yeast pheromone-response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase. Genes Dev 6: 1305-1318.
8. Chan, R.K., and Otte, C.A. (1982) Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by α-factor and alpha-factor pheromones. Cell 2: 11-20.
9. Chant, J. (1994) Cell polarity in yeast. Trends Genet 10: 328-333.
10. Chant, J., and Herskowitz, I. (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65: 1203-1212.
11. Chien, C., Bartel, P.L., Sternglanz, R., and Fields, S. (1991) The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci USA 88: 9578-9582.
12. Choi, K.-Y., Satterberg, B., Lyons, D., and Elion, E.A. (1994) Ste5 Tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell 78: 499-512.
13. Chvatchko, E., Howald, I., and Riezman, H. (1986) Two yeast mutants defective in endocytosis are defective in pheromone response. Cell 46: 355-364.
14. Ciejek, E., and Thorner, J. (1979) Recovery of S. cerevisiae a-cells from G1 arrest by α-factor pheromone requires endopeptidase action. Cell 18, 623-635.
15. Clark, K.L., Dignard, D., Thomas, D.Y., and Whiteway, M. (1993) Interactions among the subunits of the G protein involved in Saccharomyces cerevisiae mating. Mol Cell Biol 13: 1-8.
16. Courchesne, W.E., Kunisawa, R., and Thorner, J. (1989) A putative protein kinase overcomes pheromone-induced arrest of cell cycle in S. cerevisiae. Cell 58: 1107-1119.
17. α-like protein implicated in the a-and α-factor response pathway. Cell 50: 1000-1010.
18. Dietzel, C., and Kurjan, J. (1987b) Pheromone regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitisation to pheromone. Mol Cell Biol 7: 4169-4177.
19. Drubin, D.G. (1991) Development of cell polarity in budding yeast. Cell 65: 1093-1096.
20. +/CDC28-related kinase required for transition from mitosis into conjugation. Cell 60: 649-664.
21. Feilotter, H.E., Hannon, G.J., Ruddell, C.J., and Beach, D. (1994) Construction of an improved host strain for two hybrid screening. Nucl Acids Res 22: 1502-1503.
22. Fields, S., and Song, O.K. (1989) A novel genetic system to detect protein-protein interactions. Nature 340: 245-246.
23. Harper, J.W., Adami, G.R., Wei, N., Keyomarsi, K., and Elledge, S.J. (1993) The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816.
24. Hasson, M.S., Blinder, D., Thorner, J., and Jenness, D.D. (1994) Mutational activation of the STE5 gene product bypasses the requirement for G protein β and gamma subunits in the yeast pheromone-response pathway. Mol Cell Bioll 4: 1054-1065.
25. Herskowitz, I. (1995) MAP kinase pathways in yeast: for mating and more. Cell 80: 187-197.
26. Hill, C.S., and Treisman, R. (1995) Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell 80: 199-211.
27. Hill, J., Myers, A.M., Koerner, T.J., and Tzagoloff, A. (1986) Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167.
28. α subunit involved in yeast mating, is implicated in interactions with the pheromone receptors. Genes Dev 5: 467-474.
29. Jenness, D.D., and Spartick, P. (1986) Downregulation of the α-factor pheromone receptor in Saccharomyces cerevisiae. Cell 46: 345-353.
30. Konopka, J.B., Jenness, D.D., and Hartwell, L.H. (1988) The C-terminus of the S. cerevisiae α-pheromone receptor mediates an adaptive response to pheromone. Cell 54: 609-620.
31. Leberer, E., Dignard, D., Harcus, D., Thomas, D.Y., and Whiteway, M. (1992) The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein β gamma subunits to downstream signalling components. EMBO J 11: 4815-4824.
32. MacKay, V.L., Welch, S.K., Insley, M.Y., Manney, T.R., and Holly, J. (1988) The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin. Proc Natl Acad Sci USA 85: 55-59.
33. Marcus, S., Polverino, A., Barr, M., and Wigler, M. (1994) Complex between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc Natl Acad Sci USA 91: 7762-7766.
34. Marsh, L., Neiman, A.M., and Herskowitz, I. (1991) Signal transduction during pheromone response in yeast. Annu Rev Cell Biol 7: 699-728.
35. Marshall, C.J. (1995) Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation. Cell 80: 179-185.
36. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
37. Miyajima, I., Nakayama, N., Brenner, C., and Miyajima, A. (1987) GPA1, a haploid-specfic essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 50: 1011-1019.
38. Val-50 mutation in the mating-factor signalling pathway in Saccharomyces cerevisiae. Mol Cell Biol 9: 2289-2297.
39. Nakafuku, M., Itoh, H., Nakamura, S., and Kaziro, Y. (1987) Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G protein. Proc Natl Acad Sci USA 84: 2140-2144.
40. Nakayama, N., Kaziro, Y., Arai, K.-I., and Matsumoto, K. (1988) Role of STE genes in the mating factor signaling pathway mediated by GPA1 in Saccharomyces cerevisiae. Mol Cell Biol 8: 3777-3783.
41. + gene, essential for sexual differentiation of Schizosaccharomyces pombe, encodes a protein with a leucine zipper motif. Nucl Acids Res 19: 7043-7047.
42. Pringle, J.R., Preston, R.A., Adams, A.E.M., Stearns, T., Drubin, D.G., Haarer, B.K., and Jones, E.W. (1989) Fluoresence microscopy methods for yeast. Meth Cell Biol pp. 357-435.
43. Printen, J.A., and Sprague, G.F., Jr. (1994) Protein-protein interactions in the yeast pheromone-response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics 138: 609-619.
44. Ramer, S.W., and Davis, R.W. (1993) A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 90: 452-456.
45. Ramezani Rad, M., Lützenkirchen, K., Xu, G., Kleinhans, U., and Hollenberg, C.P. (1991) The complete sequence of a 11 953 bp fragment from C1G on chromosome III encompasses four open reading frames. Yeast 7: 533-538.
46. Ramezani Rad, M., Xu, G., and Hollenberg, C.P. (1992) STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae. Mol Gen Genet 236: 145-154.
47. Reneke, J.E., Blumer, K.J., Courchesne, W.E., and Thorner, J. (1988) The carboxy-terminal segment of the yeast α-factor receptor is a regulatory domain. Cell 55: 221-234.
48. Rhodes, N., Connell, L., and Errede, B. (1990) STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast. Genes Dev 4: 1862-1874.
49. Rothstein, R.J. (1983) One-step gene disruption in yeast. Meth Enzymol 101: 202-211.
50. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
51. Sena, E.P., Radia, D.N., and Fogel, S. (1973) Synchronous mating in yeast. Proc Natl Acad Sci USA 70: 1373-1377.
52. Sherman, F., Fink, G.R., and Hicks, J.B. (1986) Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
53. Sprague, G.F., Jr., and Herskowitz, I. (1981) Control of yeast cell type by the mating type locus I. Identification and control of expression of the a-specific gene, BAR1. J Mol Biol 153: 305-321.
54. Sprague, G.F., Jr., and Thorner, J. (1992) Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae. In The Molecular Biology of the Yeast Saccharomyces cerevisiae: Gene Expression. Broach, J., Jones, E., and Pringle, J.R. (eds). Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp. 657-744.
55. Sprague, G.F., Jr., Jensen, R., and Herskowitz, I. (1983) Control of cell type by the mating type locus: positive regulation of the α-specific STE3 gene by the MATa1 product. Cell 32: 409-413.
56. Stevenson, B.J., Rhodes, N., Errede, B., and Sprague, G.F., Jr. (1992) Constitutive mutants of the protein kinase STE11 activate the yeast pheromone-response pathway in the absence of the G protein. Genes Dev 6: 1293-1304.
57. Stone, D.E., and Reed, S.I. (1990) G protein mutations that alter the pheromone response in Saccharomyces cerevisiae. Mol Cell Biol 10: 4439-4446.
58. Teague, M.A., Chaleff, D.T., and Errede, B. (1986) Nucleotide sequence of the yeast regulatory gene STE7 predict a protein homologous to protien kinases. Proc Natl Acad Sci USA 83: 7371-7375.
59. Whiteway, M., Hougan, L., Dignard, D., Thomas, D.Y., Bell, L., Saari, G.C., Grant, F.J., O'Hara, P., and MacKay, V.L. (1989) The STE4 and STE18 genes of yeast encode potential β and gamma subunits of the mating factor receptor-coupled G protein. Cell 56: 467-477.
60. Whiteway, M., Hougan, L., and Thomas, D.Y. (1990) Overexpression of the STE4 gene leads to mating response in haploid Saccharomyces cerevisiae. Mol Cell Biol 10: 217-222.
61. Whiteway, M., Clark, K.L., Leberer, E., Dignard, D., and Thomas, D.Y. (1994) Genetic identification of residues involved in association of α and β G-protein subunits. Mol Cell Biol 14: 3223-3229.
62. Wu, C., Whiteway, M., Thomas, D.Y., and Leberer, E. (1995) Molecular characterisation of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J Biol Chem 270: 15984-15992.