Failure of the cystic fibrosis transmembrane conductance regulator to conduct ATP

Science

Volumn 271, Issue 5257, 1996, Pages 1876-1879

  Reddy, M M ^a,b   Quinton, P M ^a,b   Haws, C ^a,b   Wine, J J ^a,b   Grygorczyk, R ^a,b   Tabcharani, J A ^a,b   Hanrahan, J W ^a,b   Gunderson, K L ^a,b   Kopito, R R ^a,b  

^a STANFORD UNIVERSITY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CHLORIDE CHANNEL; CYCLIC AMP DEPENDENT PROTEIN KINASE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; APICAL MEMBRANE; ARTICLE; CELL MEMBRANE TRANSPORT; CYSTIC FIBROSIS; EPITHELIUM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; LIPID BILAYER; LUNG ALVEOLUS CELL; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; MAMMALIA;

EID: 0029998981     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5257.1876     Document Type: Article

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23. 4 (pH 7.3), Measurements were made at 35° ± 2°C. Constant current pulses of 50 nA and 0.5-s duration were applied through one barrel of a double cannula perfusion pipette Luminal perfusates were introduced, and luminal potentials were measured through the other barrel
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25. Standard single-channel and whole-cell patchclamp methods were used [O P. Hamill, A. Marty, E. Neher, B. Sakmann, F. J. Sigworth, Pfiueg. Arch. Eur. J. Physiol 391, 85 (1981)]. In whole-cell experiments, 500-ms voltage steps of -112 to +88 mV were applied in 10-mV increments every 2 s from the holding level of 0 mV The pClamp 6 software and TL-1 DMA interface (Axon Instruments) were used to generate the command potentials and for data acquisition. When the pipette or bath (or both) contained ATP, electrical connection to the Ag-AgCl electrode was made through an agar bridge containing NaCl solution Liquid junction potentials were measured against a flowing 3 M KCI electrode [E Neher, Methods Enzymol 207, 123 (1992)]. All voltages shown were corrected for liquid junction potentials.
26. Standard single-channel and whole-cell patchclamp methods were used [O P. Hamill, A. Marty, E. Neher, B. Sakmann, F. J. Sigworth, Pfiueg. Arch. Eur. J. Physiol 391, 85 (1981)]. In whole-cell experiments, 500-ms voltage steps of -112 to +88 mV were applied in 10-mV increments every 2 s from the holding level of 0 mV The pClamp 6 software and TL-1 DMA interface (Axon Instruments) were used to generate the command potentials and for data acquisition. When the pipette or bath (or both) contained ATP, electrical connection to the Ag-AgCl electrode was made through an agar bridge containing NaCl solution Liquid junction potentials were measured against a flowing 3 M KCI electrode [E Neher, Methods Enzymol 207, 123 (1992)]. All voltages shown were corrected for liquid junction potentials.
27. We thank S.-X. Zheng and J. Liao for technical assistance. Supported by grants from the National Institutes of Health (DK43994 to R.R K HL42368 and DK45913 to J.J.W., and DK41329 to P.M.Q.), the Canadian and U.S. Cystic Fibrosis Foundations, the Medical Research Council (Canada), Cystic Fibrosis Research, Inc , and gifts from Patricia Bresee and Kay and Ronald Presnell This work was done during the tenure of an Established Investigatorship of the American Heart Association (R R K), a U.S. Cystic Fibrosis Foundation postdoctoral fellowship (K L.G.), and a Medical Research Council Scientist award (J.W.H.).