Quantitative RT-PCR on CYP1A1 heterogeneous nuclear RNA: A surrogate for the in vitro transcription run-on assay

BioTechniques

Volumn 20, Issue 3, 1996, Pages 470-477

  Elferink, Cornelis J ^a   Reiners Jr , John J ^a  

^a WAYNE STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,3,7,8 TETRACHLORODIBENZO PARA DIOXIN; COMPLEMENTARY RNA; DIMETHYL SULFOXIDE; NUCLEAR RNA;

ANIMAL CELL; ARTICLE; GENETIC TRANSCRIPTION; KERATINOCYTE; MOLECULAR CLONING; MOUSE; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION INITIATION;

EID: 0029998709     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/19962003470     Document Type: Article

References (19)

1. Alterman, R-B.M., S. Ganguly, D.H. Schulze, W.F. Marzluff, C.L. Schildkraut and A.I. Skoultchi, 1984. Cell cycle regulation of mouse H3 histone mRNA metabolism. Mol Cell. Biol. 4:123-132.
2. Aviv, H. and P. Leder. 1972. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69:1408-1413.
3. Chang, C-D., P. Phillips, K.E. Lipson, V.J. Cristofalo and R. Baserga. 1991. Senescent human fibroblasts have a post-transcriptional block in the expression of the proliferating cell nuclear antigen gene. J. Biol. Chem. 206:8663-8666.
4. Chirgwin, J.M., A.E. Przybyla, R.J. MacDonald and W.J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonucleases. Biochemistry 75:5294-5299.
5. Elferink, C.J., S. Sassa and B.K. May. 1988 Regulation of 5-aminolevulinate synthase in mouse erythroleukemic cells is different from that in liver. J. Biol. Chem. 263:13012-13016.
6. 28K pre-messenger RNA sequences by the polymerase chain reaction. Biochem. Biophys. Res. Comm. 168:430-436.
7. Green, M.R. 1991. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annu. Rev. Cell Biol. 7.559-599.
8. 1-450 gene transcription by 2,3,7,8-tetrachlorodibenzo-p-dioxin in wild type and variant mouse hepatoma cells. J. Biol. Chem. 259:5400-5402.
9. 1450 gene expression by dioxin. Science 227:1499-1502.
10. Negishi, M., D.C. Swan, L.W. Enquist and D.W. Nebert 1981. Isolation and characterization of a cloned DNA sequence associated with the murine Ah locus and 3-methylcholanthrene-induced form of cytochrome P-450. Proc. Natl Acad. Sci. USA 78:800-804.
11. Owczarek, C.M., P. Enriquez-Harris and N. Proudfoot. 1992. The primary transcription unit of the human α2 globin gene defined by quantitative RT/PCR. Nucleic Acids Res. 20:851-858.
12. Reiners, JJ. Jr., A.R. Cantu, G. Thai and A. Schöller. 1992. Differential expression of basal and hydrocarbon-induced cytochrome P450 monooxygenase and quinone reductase activities in subpopulations of murine epidermal cells differing in their stages of differentiation. Drug Metabol. Dispos. 20.360-366.
13. Reiners, J.J. Jr., A. Schöller, P. Bischer, A.R. Cantu and A. Pavone. 1993. Suppression of cytochrome P450 Cyp1a-1 induction in murine hepatoma 1c1c7 cells by 12-O-tetradecanoylphorbol-13-acetate and inhibitors of protein kinase C. Arch. Biochem. Biophys. 307:449-454.
14. Trapnell, B.C. 1993. Quantitative evaluation of gene expression in freshly isolated human respiratory epithelial cells. Am. J. Physiol. 264:L199-L212.
15. Vanden Heuvel, J.P, F.L. Tyson and D.A. Bell. 1993. Construction of recombinant RNA templates for use as internal standards in quantitative RT-PCR. BioTechniques 74:395-398.
16. 1-acid glycoprotein: evidence for hormone-regulated RNA processing. Biochemistry 81:4241-4245.
17. Wang, A.M., M.V. Doyle and D.F. Mark. 1989. Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 80:9717-9721.
18. Whitlock, J.P. Jr. 1987. The regulation of gene expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Pharmacol Rev, 39:147-161.
19. Yang, M. and M. Kurkinen. 1994. Different mechanisms of regulation of the human stromelysin and collagenasc genes. Eur. J. Biochem. 222:651-658.