Assembly of T7 capsids from independently expressed and purified head protein and scaffolding protein

Journal of Molecular Biology

Volumn 258, Issue 2, 1996, Pages 286-298

  Cerritelli, Mario E ^a,b   Studier, F William ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES   (United States)

Author keywords

Assembly from purified proteins; Bacteriophage T7; Capsids

Indexed keywords

CELL PROTEIN; DEXTRAN; MACROGOL; MONOMER; TETRAMER;

ARTICLE; BACTERIOPHAGE T7; CONTROLLED STUDY; GEL ELECTROPHORESIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; VIRUS CAPSID;

EID: 0029998659     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0250     Document Type: Article

References (33)

1. Bazinet C. & King, J. (1985). The DNA translocating vertex of dsDNA bacteriophage. Annu. Rev. Microbiol. 39, 109-129.
2. Black, L. W. (1989). DNA packaging in dsDNA bacteriophages. Annu. Rev. Microbiol. 43, 267-292.
3. Casjens, S. & Hendrix, R. (1988). Control mechanisms in dsDNA bacteriophage assembly. In The Bacteriophages (Calendar, R., ed.), vol. 1, pp. 15-92, Plenum, New York.
4. Cerritelli, M. E. (1991). Purification of T7 head, scaffolding, and connector proteins expressed from cloned genes, and assembly of T7 prohead shells from the purified proteins. PhD thesis, State University of New York at Stony Brook.
5. Cerritelli, M. E. & Studier, F. W. (1996). Purification and characterization of T7 head-tail connectors expressed from the cloned gene. J. Mol. Biol. 258, 299-307.
6. Condron, B. G., Atkins, J. F. & Gesteland, R. F. (1991). Frameshifting in gene 10 of bacteriophage T7. J. Bacteriol. 173, 6988-7003.
7. de la Campa, A. G., Kale, P., Springhorn, S. S. & Lacks, S. A. (1987). Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease. J. Mol. Biol. 196, 457-469.
8. Dunn, J. J. & Studier, F. W. (1983). Complete nucleotide sequence of bacteriophage T7 DNA and the location of T7 genetic elements. J. Mol. Biol. 166, 477-535.
9. Earnshaw, W. C. & Casjens, S. R. (1980). DNA packaging by double-stranded DNA bacteriophages. Cell, 21, 319-331.
10. Guo, P., Erickson, S., Xu, W., Olson, N., Baker, T. S. & Anderson, D. (1991). Regulation of the phage φ29 prohead shape and size by the portal vertex. Virology, 183, 366-373.
11. Hausmann, R. & LaRue, K. (1969). Variations in sedimentation patterns among deoxyribonucleic acids synthesized after infection of Escherichia coli by different amber mutants of bacteriophage T7. J. Virol. 3, 278-281.
12. Masker, W. E. & Serwer, P. (1982). DNA packaging in vitro by an isolated bacteriophage T7 procapsid. J. Virol. 43, 1138-1142.
13. Mosesson, M. W., Hainfeld, J., Wall, J. & Haschemeyer, R. H. (1981). Identification and mass analysis of human fibrinogen molecules and their domains by scanning transmission electron microscopy. J. Mol. Biol. 153, 695-718.
14. Prevelige, P. E., Jr, Thomas D. & King, J. (1988). Scaffolding protein regulates the polymerization of P22 coat subunits into icosahedral shells in vitro. J. Mol. Biol. 202, 743-757.
15. Roeder, G. S. & Sadowski, P. D. (1977). Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage. Virology, 76, 263-285.
16. Rosenberg, A. H., Lade, B. N., Chui, D., Lin, S., Dunn, J. J. & Studier, F. W. (1987). Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene, 56, 125-135.
17. Serwer, P. (1976). Internal proteins of bacteriophage T7. J. Mol. Biol. 107, 271-291.
18. Serwer, P. (1979). Fibrous projections from the core of a bacteriophage T7 procapsid. J. Supramol. Struct. 11, 321-326.
19. Serwer, P. & Pichler, M. E. (1978). Electrophoresis of bacteriophage T7 and T7 capsids in agarose gels. J. Virol. 28, 917-928.
20. Serwer, P. & Watson, R. H. (1982). Function of an internal bacteriophage T7 core during assembly of a T7 procapsid. J. Virol. 42, 595-601.
21. Serwer, P., Watson, R. H. & Hayes, S. J. (1982). Detection and characterization of agarose-binding, capsid-like particles produced during assembly of a bacteriophage T7 procapsid. J. Virol. 42, 583-594.
22. Serwer, P., Masker, W. E. & Allen, J. L. (1983). Stability and in vitro DNA packaging of bacteriophages: effects of dextrans, sugars and polyols. J. Virol. 45, 665-671.
23. Steven, A. C. & Trus, B. L. (1986). The structure of bacteriophage T7. In Electron Microscopy of Proteins 5, Viral Structure, pp. 1-35, Academic Press, New York.
24. Steven, A. C., Serwer, P., Bisher, M. E. & Trus, B. L. (1983). Molecular architecture of bacteriophage T7 capsid. Virology, 124, 109-120.
25. Studier, F. W. (1972). Bacteriophage T7. Science, 176, 367-376.
26. Studier, F. W. (1991). Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J. Mol. Biol. 219, 37-44.
27. Studier, F. W. & Hausmann, R. (1969). Integration of two sets of T7 mutants. Virology, 39, 587-588.
28. Studier, F. W. & Maizel, J. V., Jr (1969). T7 directed protein synthesis. Virology, 39, 575-586.
29. Studier, F. W. & Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
30. Studier, F. W., Rosenberg, A. H., Dunn, J. D. & Dubendorff, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60-89.
31. Wall, J. S. (1979). Biological scanning transmission electron microscopy. In Introduction to Analytical Electron Microscopy (Hren, J. J., Goldstein, J. I. & Joy, D. C., eds), pp. 333-342, Plenum, New York.
32. Wall, J. S. & Hainfeld, J. F. (1986). Mass mapping with the scanning transmission electron microscope. Annu. Rev. Biophys. Biophys. Chem. 15, 355-376.
33. Wall, J. S., Hainfeld, J. F. & Chung, K. D. (1985). Films that wet without glow discharge. In Proc. 43rd Ann. Mtg. EMSA (Bailey, G. W, ed.), pp. 716-717, San Francisco Press, San Francisco.