A NusG-like protein from Thermotoga maritima binds to DNA and RNA

Journal of Bacteriology

Volumn 178, Issue 14, 1996, Pages 4089-4098

  Liao, Daiqing ^a,c   Lurz, Rudi ^b   Dobrinski, Beate ^b   Dennis, Patrick P ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA; DOUBLE STRANDED DNA; ENDONUCLEASE; ETHIDIUM BROMIDE; RNA; RNA POLYMERASE; SINGLE STRANDED DNA; TRANSCRIPTION FACTOR;

ARTICLE; BINDING AFFINITY; DNA PROTEIN COMPLEX; ELECTRON MICROSCOPY; ESCHERICHIA COLI; EUBACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN RNA BINDING; PROTEIN VARIANT; SEQUENCE HOMOLOGY; THERMOPHILIC BACTERIUM;

EID: 0029998144     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.14.4089-4098.1996     Document Type: Article

References (43)

1. Achenbach-Richter, L., R. Gupta, K. O. Stetter, and C. Woese. 1987. Were the original eubacteria thermophiles? Syst. Appl. Microbiol. 9:34-39.
2. An, G., and J. Friesen. 1980. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene 12:33-39.
3. Bremer, H., and P. P. Dennis. 1987. Modulation of chemical composition and other parameters of cell by growth rate, p. 1527-1542. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
4. Brennan, C. A., A. J. Dombroski, and T. Platt. 1987. Transcription termination factor p is an RNA-DNA helicase. Cell 48:945-952.
5. Broyles, S., and D. E. Pettijohn. 1986. Interaction of the Escherichia coli HU protein with DNA. Evidence for formation of nucleosome-like structures with altered DNA helical pitch. J. Mol. Biol. 187:47-61.
6. Christiansen, G., L. B. Petersen, J. E. Koehler, A. G. Lundemose. and S. Birkelund. 1993. Interaction between the Chlamydia trachomatis histone-like protein (Hcl) and DNA. J. Bacteriol. 175:1785-1795.
7. Das, A. 1992. How the phage lambda N gene product suppresses transcription termination: communication of RNA polymerase with regulatory proteins mediated by signals in the nascent RNA. J. Bacteriol. 174:6711-6716.
8. Downing, W., and P. Dennis. 1987. Transcription products from the rplKALJrpoBC gene cluster. J. Mol. Biol. 194:609-620.
9. Dawning, W., S. Sullivan, M. Gottesman, and P. Dennis. 1990. Sequence and transcription pattern of the essential Escherichia col: secE-nusG operon. J. Bacteriol. 172:1621-1627.
10. Drlica, K., and J. Rouviere-Yaniv. 1987. Histonelike proteins of bacteria. Microbiol. Rev. 51:301-319.
11. Friedman, D. I., and E. R. Olson. 1983. Evidence that a nucleotide sequence, "box A", is involved in the action of the NusA protein. Cell 34:143-149.
12. Greenblat, J., J. R. Nodwell, and S. W. Mason. 1993 Transcription antitermination. Nature (London) 364:401-406.
13. Heinrich, T., W. Schröder, U. Erdmann, and R. K. Hartmann. 1992. Identification of the gene encoding transcription factor NusG in Thermus thermophiius. J. Bacteriol. 174:7859-7863.
14. Higgins, C., J. Hinton, J. Hulton, T. Owen-Hughes, G. Pavitt, and A. Seirafi. 1990. Protein H1 : a role for chromatin structure in the regulation of bacterial gene expression and virulence? Mol. Microbiol. 4:2007-2012.
15. Huber, R., T. Langworthy, H. Konig, M. Thomm, C. Woese, U. Sleytr, and K. Stetter. 1986. Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch. Microbiol. 144:324-333.
16. Hulton, C., A. Seirafi, J. Hinton, J. Sidebotham, L. Waddell, G. Pavitt, T. Owen-Hughes, A. Spassky, H. Buc, and C. Higgins. 1990. Histone-like protein Hl (H-NS), DNA supercoiling, and gene expression in bacteria. Cell 63:631-642.
17. Li, J., R. Horwitz, S. McCrachen, and J. Greenblatt. 1992. NusG, a new Escherichia coli elongation factor involved in transcriptional antitermination by N protein of phage λ. J. Biol. Chem. 267:6012-6019.
18. Li, J., S. W. Mason, and J. Greenblatt. 1993. Elongation factor NusG interacts with termination factor p to regulate termination and antitermination of transcription. Genes Dev. 7:161-172.
19. 18a.Liao, D. Unpublished data.
20. Liao, D., and P. P. Dennis. 1992. The organization and expression of essential translation, transcription component genes in the extremely thermophilic eubacterium Thermotoga maritima. J. Biol. Chem. 267:22787-22797.
21. Linn, T., and J. Greenblatt. 1992. The NusA and NusG proteins of Escherichia coli increase the in vitro read through frequency of a transcriptional attenuator preceding the gene for b subunit of RNA polymerase. J. Biol. Chem. 264:22787-22797.
22. 20a.Lurz, R., B. Dobrinski, D. Liao, and P. D. Dennis. Unpublished results.
23. Mason, S. W., and J. Greenblatt. 1991. Assembly of transcription elongation complexes containing the N-protein of phage X and Escherichia coli elongation factors NusA, NusB, NusG, and S10. Genes Dev. 5:1504-1512.
24. Miyake, K., H. Onaka, S. Horinouchi, and T. Beppu. 1994. Organization and nucleotide sequence of the secE-nusG region of Streptomyces griseus. Biochim. Biophys. Acta 1217:97-100.
25. Morgan, W. D., D. G. Bear, and P. H. Von Hippel. 1984. Specificity of release by Escherichia coli transcription termination factor Rho of nascent mRNA transcripts initiated at the λ pR promoter. J. Biol. Chem. 259:8664-8671.
26. Morgan, W. D, D. G. Bear, and P. H. Von Hippel. 1983. p-dependent termination of transcription. 1. Identification and characterization of termination sites for transcription from the bacteriophage λ pR promoter. J. Biol. Chem. 258:9553-9564.
27. Nodwell, J. R., and J. Greenblatt. 1993. Recognition of box A antiterminator RNA by the E. coli antitermination factor NusB and ribosomal protein S10. Cell 72:261-268.
28. Okamoto, S., T. Nihira, H. Kataoka, A. Suziki, and V. Yamada. 1992. Purification and molecular cloning of a butyrolactone autoregulator receptor from Streptomyces Virginiae. J. Biol. Chem. 267:1093-1098.
29. Palm, P., C. Schleper, I. Arnold-Ammer, L Holz, F. Lollspeich, and W. Zillig. 1993. The DNA-dependent RNA polymerase of Thermotoga maritima: characterization of the enzyme and the DNA sequence of the genes for the large subunits. Nucleic Acids Res. 20:4904-4908.
30. Post, L. E., G. D. Strycharz, M. Nomura, H. Lewis, and P. P. Dennis. 1979. Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit in Escherichia coli. Proc. Natl. Acad. Sci. USA 76:1697-1701.
31. Roberts, J. W. 1993. RNA and protein elements of E. coli and λ transcription antitermination complexes. Cell 72:653-655.
32. Rosenberg, M., D. Court, H. Shimatake, C. Brady, and D. L. Wulff. 1978. The relationship between function and DNA sequence in an incistronic regulatory region of phage λ. Nature (London) 272:414-422.
33. Rouvière-Yaniv, J., M. Yaniv, and J.-E. Germond. 1979. E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNA. Cell 17:265-274.
34. Sambrook, J., E. F. Fritch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Schatz, P., P. Riggs, A. Jacq, M. Fath, and J. Beckwith. 1989. The secE gene encodes an integral membrane protein required for protein export in E. coli. Genes Dev. 3:1035-1044.
36. Silhavy, T. J., M. L. Herman, and L. Enquist. 1984. Experiments with gene fusions, p. 107-112. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
37. Spiess, E., and R. Lurz. 1988. Electron microscopic analysis of nucleic acids and nucleic acid-protein complexes. Methods Microbiol. 20:293-323.
38. Studier, F. W., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 105:60-89.
39. Sullivan, S., and M. E. Gottesman. 1992. Requirement for E. coli NusG protein in factor-dependent transcription termination. Cell 68:989-994.
40. Sullivan, S., D. Ward, and M. E. Gottesman. 1992. Effect of Escherichia coli nusG function on λ N-mediated transcription antitermination. J. Bacteriol. 174:1339-1224.
41. Whalen, W., and A. Das. 1988. NusA protein is necessary and sufficient in vitro for phage λ N gene product to suppress a rho-independent terminator placed downstream of nutL. Proc. Natl. Acad. Sci. USA 85:2494-2498.
42. Yager, T. D., and P. H. von Hippel. 1987. Transcription elongation and termination in Escherichia coli, vol. 2, p. 1241-1275. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
43. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nuclcotide sequences of the M13 mp18 and pUC19 vectors. Gene 33:103-119.