Immunohistochemical bulk staining of slides using a rack peroxidase-labelled streptavidin-biotin technique

Applied Immunohistochemistry and Molecular Morphology

Volumn 4, Issue 2, 1996, Pages 135-138

  Pfeiffer, Per ^a   Grabau, Dorthe A ^a   Nielsen, Ole ^a   Clausen, Per P ^a  

^a ODENSE UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Bulk staining; Immunohistochemistry; Labelled streptavidin biotin; Time consumption

Indexed keywords

ARTICLE; ECONOMIC ASPECT; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; TECHNIQUE;

EID: 0029997881     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (6)

1. Taylor CR. An exaltation of experts: concerted efforts in the standardization of immunohistochemistry. Hum Pathol 1994;25:2-11.
2. Elias JM, Margiotta M, Gaborc D. Sensitivity and detection efficiency of the peroxidase anti-peroxidase (PAP), avidin-biotin peroxidase complex (ABC), and peroxidase-labeled avidin-biotin (LAB) methods. Am J Clin Pathol 1989;92: 62-7.
3. Bindl JM, Warnke RA. Advantages of detecting monoclonal antibody binding to tissue sections with biotin and avidin reagents in Coplin jars. Am J Clin Pathol 1986;85:490-3.
4. Stross WP, Jones M, Mason DY. Automation of APAAP immunocytochemical technique. J Clin Pathol 1989;42:106-12.
5. Sofroniew MV, Schrell U. Long-term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility, and convenience of single- and two-color light microscopic immunocytochemistry. J Histochem Cytochem 1982;30:504-11.
6. Tacha D, Brown M, Galey WT. A two step immunoperoxidase detection system with repeated use of diluted antibodies for bulk staining of slides. J Histotechnol 1990;13:15-21.