Variation in recovery of motile sperm after preparation by a simple Percoll gradient technique

Journal of Assisted Reproduction and Genetics

Volumn 13, Issue 6, 1996, Pages 485-488

  Miller, Kurt F ^a   Falcone, Tommaso ^a   Goldberg, Jeffrey M ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

Author keywords

in vitro fertilization; motility; Percoll; semen preparation; sperm function

Indexed keywords

ARTICLE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; FERTILIZATION IN VITRO; HUMAN; HUMAN TISSUE; MALE; PRIORITY JOURNAL; RETROSPECTIVE STUDY; SPERMATOZOON MOTILITY; TECHNIQUE;

CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; FERTILIZATION; FERTILIZATION IN VITRO; HUMANS; MALE; POVIDONE; SEMEN; SILICON DIOXIDE; SPERM COUNT; SPERM MOTILITY; SPERMATOZOA;

EID: 0029996922     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02066529     Document Type: Article

References (10)

1. Sun LS, Gastaldi C, Peterson EM, de la Maza LM, Stone SC: Comparison of techniques for the selection of bacteria-free sperm preparations. Fertil Steril 1987;48:659-663
2. McClure RD, Nunes L, Tom R: Semen manipulation: Improved sperm recovery and function with a two-layer Percoll gradient. Fertil Steril 1989;51:874-877
3. Ng FLH, Liu DY, Baker HWG: Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples. Hum Reprod 1992;7:261-266
4. Englert Y, Van den Bergh M, Rodesh C, Bertrand E, Biramane J, Legreve A: Comparative auto-controlled study between swim-up and Percoll preparation of fresh semen samples for in vitro fertilization. Hum Reprod 1992;7:399-402
5. Miller KF, Goldberg JM, Collins RL: Covering embryo cultures with mineral oil alters embryo growth by acting as a sink for an embryotoxic substance. J Assist Reprod Genet 1994;11:342-345
6. SAS Institute Inc.: SAS User's Guide: Statistics, Version 5 Edition. Cary NC, SAS Institute Inc., 1985
7. Van Der Zwalmen P, Bertin-Segal G, Geerts L, Debauche C, Schoysman R: Sperm morphology and IVF pregnancy rate: Comparison between Percoll gradient centrifugation and swim-up procedures. Hum Reprod 1991;6:581-588
8. Ord T, Patrizio P, Marello E, Balmaceda JP, Asch RH: Mini-Percoll: A new method of semen preparation for in vitro fertilization in severe male factor infertility. Hum Reprod 1990; 5:987-989
9. Bolton VN, Warren ER, Braude PR: Removal of bacterial contaminants from semen for in vitro fertilization or artificial insemination by the use of buoyant density centrifugation. Fertil Steril 1986;46:1128-1132
10. Mortimer D: Sperm preparation techniques and iatrogenic failure of in vitro fertilization. Hum Reprod 1991;6:173-176