Evaluation of agonist efficacy and receptor reserve for α2d-adrenergic receptor regulated g protein activation in pc12 cell membranes

Pharmacology

Volumn 52, Issue 4, 1996, Pages 252-262

  Tian, Wang Ni ^a   Duzic, Emir ^b   Deth, Richard C ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Epinephrine; G proteins; Guanosine 5 triphosphate; Rauwolscine; Receptor reserve; 2 Adrenergic receptors

Indexed keywords

ADRENALIN; ALPHA 2 ADRENERGIC RECEPTOR; ALPHA 2 ADRENERGIC RECEPTOR BLOCKING AGENT; ALPHA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; DIHYDROQUINOLINE DERIVATIVE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); MEMBRANE RECEPTOR; RAUWOLSCINE; RECEPTOR RESERVE; SULFUR 35; TRITIUM;

ANIMAL CELL; ARTICLE; BINDING KINETICS; CELL MEMBRANE; CELL STRAIN; CONTROLLED STUDY; DISSOCIATION CONSTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN INDUCTION; RADIOASSAY; RAT; RECEPTOR BINDING; RECEPTOR DENSITY;

ADRENERGIC ALPHA-AGONISTS; ANIMALS; ENZYME ACTIVATION; EPINEPHRINE; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); PC12 CELLS; QUINOLINES; RATS; RECEPTORS, ADRENERGIC, ALPHA-2; SIGNAL TRANSDUCTION;

EID: 0029995339     PISSN: 00317012     EISSN: 14230313     Source Type: Journal    
DOI: 10.1159/000139390     Document Type: Article

References (15)

1. Duzic E, Lanier SM: Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors, III Coupling of a;-adrenergic receptor subtypes in a cell type-specific manner, J Biol Chem 1992;267: 24045-24052.
2. Eason MG, Jacinto MT, Liggett SB: Contribution of ligand structure to activation of uj-adrcnergic receptor subtype coupling to G, Mol Pharmacol 1994;45:696-702.
3. q/n, Mol Pharmacol 1994;45:890-898.
4. Xiao RP, Xiangwu J, Lakatta EG: Functional coupling of the T-adrc-noccptor to a pertussis toxin-sensitive G protein in cardiac myocytes, Mol Pharmacol 1995;47:322-329.
5. Eason MG, Kurose H, Holt BD, Raymond JR, Liggett SB: Simultaneous coupling of ai-adrenergic receptors to two G-proteins with opposing effects, J Biol Chem 1992; 267:15795-15801.
6. Tian W-N, Duzic M, Lanier SM, Deth RC: Determinants of alpha-2-adrcnergic receptor activation of G-protcins: Evidence for a precoupled R/G state, Mol Pharmacol 1994:45: 524-531.
7. Besse JC, Furchgott RF: Dissociation constants and relative efficacies of agonists acting on alpha-adrcner-gic receptors in rabbit aorta. Pharmacol ExpTher 1976:197:66-78.
8. Munson PJ, Rodbard D: Ligand: A versatile computerized approach for characterization of ligand-binding systems, Anal Biochem 1980:107: 220-239.
9. Higashijima T, Uzu S, Nakajima T, Ross EM: Mastoparan, a peptide toxin from wasp venom, mimics receptors by activating GTP-binding regulatory proteins (G-proteins), J Biol Chem 1988;263:6491-6494.
10. Lefkowitz RJ: The subunit story thickens, Nature 1992:358:372.
11. Colucci WS, Brock TA, Gimbronc MA Jr, Alexander RW: Nonlinear relationship between ai-adrenergic receptor occupancy and norepinephrine-stimulated calcium flux in cultured vascular smooth muscle cells, Mol Pharmacol 1985:27:517-524.
12. Kenakin T: Pharmacological Analysis of Drug-Receptor Interaction, New York, Raven Press, 1993.
13. Neubig RR, Gantzos RD, Thomsen WJ: Mechanism of agonist and antagonist binding to ot;-adrenergic receptors, Evidence for a precouplcd receptor-guanine nucleotide protein complex, Biochemistry 1988:27: 2374-2384.
14. Gierschik P, Moghtader R, Straub C, Dieterich K, Jakobs KH: Signal amplification in HL-60 granulocytes: Evidence that the chemotactic peptide receptor catalytically activates guanine-nucleotidc-binding regulatory proteins in native plasma membranes, Eur J Biochem 1991; 197:725-732.
15. Müller H, Weingarten R, Ransna LA, Bokoch GM, Skiar LA: Differential amplification of antagonistic receptor pathways in neutrophils, J Biol Chem 1991;266:12939-12943.