Sequences within the VP6 molecule of bluetongue virus that determine cytoplasmic and nuclear targeting of the protein

Journal of Virology

Volumn 70, Issue 7, 1996, Pages 4779-4782

  Yi, Chong Kee ^a,b   Bansal, O M B ^c   Liu, Hong Mei ^c   Chatterjee, Subhendra ^d   Roy, Polly ^a,b,c,e  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b NATURAL ENVIRONMENT RESEARCH COUNCIL   (United Kingdom)

^c UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^d University of Alabama at Birmingham   (United States)

^e CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BLUETONGUE ORBIVIRUS; CARBOXY TERMINAL SEQUENCE; CELL FRACTIONATION; GENE EXPRESSION; IMMUNOFLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANTIGENS, VIRAL; BASE SEQUENCE; BIOLOGICAL TRANSPORT; BLUETONGUE VIRUS; CAPSID; CAPSID PROTEINS; CELL NUCLEUS; CYTOPLASM; DNA PRIMERS; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; SEQUENCE DELETION;

EID: 0029994034     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.7.4778-4782.1996     Document Type: Article

References (10)

1. Dingwall, C., and R. A. Laskey. 1991. Nuclear targeting sequences-a consensus? Trends Biochem. Sci. 16:478-481.
2. Fuerst, T., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eukarotyic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:8122-8126.
3. Hayama, E., and J. K.-K. Li. 1994. Mapping and characterization of antigenic epitopes and the nucleic acid-binding domains of the VP6 protein of bluetongue viruses. J. Virol. 68:3604-3611.
4. Kalderon, D., W. D. Richardson, A. F. Markham, and A. E. Smith. 1984. Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311:33-38.
5. Le Blois, H., T. French, P. P. C. Mertens, J. N. Burroughs, and P. Roy. 1992. The expressed VP4 protein of bluetongue virus binds GTP and is the candidate guanylyl transferase of the virus. Virology 189:757-761.
6. Lee, W. C., and T. Melese. 1989. Identification and characterization of a nuclear localization sequence-binding protein in yeast. Proc. Natl. Acad. Sci. USA 86:8808-8812.
7. Mertens, P. P. C., J. N. Burroughs, A. M. Wade-Evans, H. Le Blois, S. Oldfield, A. Basak, P. Loudon, and P. Roy. 1992. Analysis of guanylyl transferase and transmethylase activities associated with bluetongue virus cores and recombinant baculovirus-expressed core-like particles. In T. E. Walton and B. I. Osburn (ed.), Bluetongue, African horsesickness and related orbiviruses, p. 404-415. CRC Press, Inc., Boca Raton, Fla.
8. Roy, P., A. Adachi, T. Urakawa, T. F. Booth, and C. P. Thomas. 1990. Identification of bluetongue virus VP6 protein as a nucleic acid-binding protein and the localization of VP6 in virus-infected vertebrate cells. J. Virol. 64:1-8.
9. Suzuki, R., Y. Matsuura, T. Suzuki, A. Ando. J. Chiba, S. Harada, I. Saito, and M. Tatsuo. 1995. Nuclear localization of the truncated hepatitis C virus core protein with its hydrophobic C terminus deleted. J. Gen. Virol. 76:53-61.
10. Urakawa, T., D. G. Ritter, and P. Roy. 1989. Expression of largest RNA segment and synthesis of VP1 protein of bluetongue virus in insect cells by recombinant baculovirus: association of VP1 protein with RNA polymerase activity. Nucleic Acids Res. 17:7395-7401.